EC Number |
General Stability |
Reference |
---|
4.2.1.1 | 0.8 M guanidinium chloride, 24 h, 50% inactivation |
33544 |
4.2.1.1 | 4°C, 1% SDS, stable for several weeks |
33539 |
4.2.1.1 | a key role for sodium ions in increasing halotolerant enzyme stability largely through interactions with the highly ordered first Na+ hydration shell, molecular dynamics calculations, overview |
748739 |
4.2.1.1 | denaturation with guanidinium chloride |
33538 |
4.2.1.1 | dialysis against pyridine dicarboxylate, at 15°C, 98% loss of activity after 24 h |
33537 |
4.2.1.1 | enzyme appears to denature with a midpoint of a structural transition near 2.1 M denaturant guanidine hydrochloride |
650349 |
4.2.1.1 | enzyme retains conformational stability in low and high salt concentrations |
666748 |
4.2.1.1 | freezing and thawing results in formation of aggregates, unfolded at 1.5 M guanidine hydrochloride |
650163 |
4.2.1.1 | guanidine hydrochloride causes denaturation |
651081 |
4.2.1.1 | guanidine hydrochloride causes denaturation and inactivation |
651093 |