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Literature summary for 4.2.1.1 extracted from

  • Elleby, B.; Sjoeblom, B.; Tu, C.; Silverman, D.N.; Lindskog, S.
    Enhancement of catalytic efficiency by the combination of site-specific mutations in a carbonic anhydrase-related protein (2000), Eur. J. Biochem., 267, 5908-5915.
    View publication on PubMed
Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Mus musculus

Protein Variants

Protein Variants Comment Organism
E115Q site-directed mutagenesis, results in increased activity Mus musculus
E92Q site-directed mutagenesis, results in increased activity Mus musculus
R117H site-directed mutagenesis, replacement reconstructs a zinc-binding site and changes a catalytically inactive murine carbonic anhydrase-related protein to an active carbonic anhydrase Mus musculus
R94H site-directed mutagenesis, replacement reconstructs a zinc-binding site and changes a catalytically inactive murine carbonic anhydrase-related protein to an active carbonic anhydrase Mus musculus
R94H/E92Q site-directed mutagenesis Mus musculus
R94H/E92Q/I121V site-directed mutagenesis Mus musculus
R94H/E92Q/I121V/I143V/I200T site-directed mutagenesis Mus musculus
R94H/E92Q/I143V/I200T site-directed mutagenesis Mus musculus
R94H/E92Q/I200T site-directed mutagenesis Mus musculus

General Stability

General Stability Organism
guanidine hydrochloride causes denaturation Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
Cl-
-
 Mus musculus
CN-
-
 Mus musculus
I-
-
 Mus musculus
N3-
-
 Mus musculus
NCO-
-
 Mus musculus
NO3-
-
 Mus musculus
SCN-
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I121V/I143V/I200T Mus musculus
5.6
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I143V/I200T Mus musculus
5.9
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I143V Mus musculus
13
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I121V Mus musculus
15
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I200T Mus musculus
19
-
 CO2 pH 9.0, 25°C, mutant R94H Mus musculus
22
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Mus musculus
-
 CO2 + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2CO3
-
 Mus musculus CO2 + H2O
-
 r

Synonyms

Synonyms Comment Organism
carbonic anhydrase
-
 Mus musculus
carbonic anhydrase-related protein
-
 Mus musculus
CARP
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
 CO2 pH 9.0, 25°C, mutant R94H Mus musculus
0.019
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I143V Mus musculus
0.023
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q Mus musculus
0.034
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I121V Mus musculus
0.038
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I143V/I200T Mus musculus
0.039
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I121V/I143V/I200T Mus musculus
0.046
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I200T Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
 Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0065
-
 CN- pH 7.0, 10°C, double mutant R94H/E92Q Mus musculus
0.0071
-
 NCO- pH 7.0, 10°C, double mutant R94H/E92Q Mus musculus
0.12
-
 SCN- pH 7.0, 10°C, double mutant R94H/E92Q Mus musculus
0.27
-
 N3- pH 7.0, 10°C, double mutant R94H/E92Q Mus musculus
12.1
-
 NO3- pH 7.0, 10°C, double mutant R94H/E92Q Mus musculus
21.4
-
 I- pH 7.0, 10°C, double mutant R94H/E92Q Mus musculus
222
-
 Cl- pH 7.0, 10°C, double mutant R94H/E92Q Mus musculus