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Literature summary for 4.2.1.1 extracted from

  • Chirica, L.C.; Elleby, B.; Lindskog, S.
    Cloning, expression and some properties of alpha-carbonic anhydrase from Helicobacter pylori (2001), Biochim. Biophys. Acta, 1544, 55-63.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
alpha-carbonic anhydrase gene cloned and sequenced, truncated gene expressed in Escherichia coli BL21(DE3) Helicobacter pylori

General Stability

General Stability Organism
enzyme appears to denature with a midpoint of a structural transition near 2.1 M denaturant guanidine hydrochloride Helicobacter pylori

Inhibitors

Inhibitors Comment Organism Structure
2-acetylamido-1,3,4-thiadiazole-5-sulfonamide acetazolamide Helicobacter pylori
thiocyanate
-
Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
17
-
CO2 pH 8.9, 25°C Helicobacter pylori

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28280
-
amino acid sequence Helicobacter pylori
29000
-
SDS-PAGE Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Helicobacter pylori
-
CO2 + H2O
-
r

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
ATCC type strain 628572
-

Purification (Commentary)

Purification (Comment) Organism
-
Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl acetate + H2O
-
Helicobacter pylori 2-nitrophenol + acetate
-
r
4-nitrophenyl acetate + H2O
-
Helicobacter pylori 4-nitrophenol + acetate
-
r
H2CO3
-
Helicobacter pylori CO2 + H2O
-
r

Synonyms

Synonyms Comment Organism
alpha-carbonic anhydrase
-
Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
240000
-
CO2 pH 8.9, 25°C Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
Helicobacter pylori

pH Range

pH Minimum pH Maximum Comment Organism
6.2 9
-
Helicobacter pylori

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8
-
thiocyanate pH 7.5, 25°C Helicobacter pylori

pI Value

Organism Comment pI Value Maximum pI Value
Helicobacter pylori calculated from amino acid composition
-
8.8
Helicobacter pylori isoelectric focusing
-
9