EC Number   |
General Stability |
Reference |
|---|
    2.1.1.45 | -80°C, storage in 30% glycerol stabilizes enzyme |
392255 |
| 2.1.1.45 | comparatively unstable, more stable in 20% sucrose solution |
485363 |
| 2.1.1.45 | enzyme can be kept active for about 2-3 weeks in buffer F in presence of 0.001 mM methotrexate, dialysis against dilute buffer leads to rapid irreversible inactivation as does freeze-drying or simple freezing |
485383 |
| 2.1.1.45 | enzyme is not inactivated by freezing and thawing |
485359 |
| 2.1.1.45 | enzyme is significantly stabilized by dUMP |
485365 |
| 2.1.1.45 | enzyme is stabilized by addition of 20-120 mM dithiothreitol + bovine serum albumin |
485381 |
| 2.1.1.45 | freezing and freeze-drying or dialysis causes rapid inactivation |
485383 |
| 2.1.1.45 | glycerol and Triton X-100 stabilize the enzyme |
485400, 485402 |
| 2.1.1.45 | quite unstable |
485366 |
| 2.1.1.45 | stable to protein denaturants and limited proteolysis |
485381 |
