Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | 10 mM, relative activity 108% | Bos taurus | |
ATP | 0.1 mM, relative activity 102% | Bos taurus | |
EDTA | 5.0 mM, relative activity 103% | Bos taurus |
Crystallization (Comment) | Organism |
---|---|
- |
Lacticaseibacillus casei |
General Stability | Organism |
---|---|
comparatively unstable, more stable in 20% sucrose solution | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bos taurus | |
dTMP | - |
Bos taurus | |
dTMP | - |
Enterococcus faecalis | |
dTMP | - |
Gallus gallus | |
thymidine phosphate | - |
Bos taurus | |
thymidine phosphate | - |
Enterococcus faecalis | |
thymidine phosphate | - |
Escherichia coli | |
thymidine phosphate | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
2'-deoxyuridylate | - |
Escherichia coli | |
0.0051 | - |
2'-deoxyuridylate | in presence of 10 mM Mg2+ | Lacticaseibacillus casei | |
0.0057 | - |
2'-deoxyuridylate | - |
Enterococcus faecalis | |
0.0075 | - |
2'-deoxyuridylate | - |
Gallus gallus | |
0.009 | - |
2'-deoxyuridylate | - |
Bos taurus | |
0.014 | - |
2'-deoxyuridylate | Ehrlich ascites carcinoma cells | Mus musculus | |
0.018 | - |
2'-deoxyuridylate | phage infected E. coli | Escherichia phage T2 | |
0.02 | - |
2'-deoxyuridylate | less purified enzyme | Bos taurus | |
0.03 | - |
2'-deoxyuridylate | phage infected E. coli | Enterobacteria phage T6 | |
0.037 | - |
2'-deoxyuridylate | Ehrlich ascites carcinoma cells | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Gallus gallus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Bos taurus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Enterococcus faecalis | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia phage T2 | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Enterobacteria phage T6 | - |
dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Enterobacteria phage T6 | - |
- |
- |
Enterococcus faecalis | - |
- |
- |
Escherichia coli | - |
- |
- |
Escherichia phage T2 | - |
- |
- |
Gallus gallus | - |
chicken | - |
Lacticaseibacillus casei | - |
- |
- |
Mus musculus | - |
Ehrlich ascites carcinoma cells | - |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
- |
Bos taurus |
Ehrlich ascites carcinoma cells | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Gallus gallus | - |
thymus | - |
Mus musculus | - |
thymus | thymus gland | Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00035 | - |
- |
Bos taurus |
54 | - |
- |
Enterococcus faecalis |
150 | - |
- |
Lacticaseibacillus casei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Gallus gallus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Gallus gallus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Enterococcus faecalis | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Enterococcus faecalis | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia phage T2 | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia phage T2 | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Enterobacteria phage T6 | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Enterobacteria phage T6 | dihydrofolate + dTMP | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
unstable above | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 6.8 | - |
Lacticaseibacillus casei |
6.5 | 6.8 | phosphate buffer | Bos taurus |
6.6 | - |
- |
Bos taurus |
7.5 | - |
- |
Gallus gallus |
7.5 | 8 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | - |
Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
dTMP | - |
Enterococcus faecalis | |
0.059 | - |
dTMP | - |
Bos taurus | |
0.14 | - |
dTMP | - |
Gallus gallus |