Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.45 extracted from

  • Horinishi, H.; Greenberg, D.M.
    Purification and properties of thymidylate synthase from calf thymus (1972), Biochim. Biophys. Acta, 258, 741-752.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
(NH4)2SO4 10 mM, relative activity 108% Bos taurus
ATP 0.1 mM, relative activity 102% Bos taurus
EDTA 5.0 mM, relative activity 103% Bos taurus

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Lacticaseibacillus casei

General Stability

General Stability Organism
comparatively unstable, more stable in 20% sucrose solution Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Bos taurus
dTMP
-
Bos taurus
dTMP
-
Enterococcus faecalis
dTMP
-
Gallus gallus
thymidine phosphate
-
Bos taurus
thymidine phosphate
-
Enterococcus faecalis
thymidine phosphate
-
Escherichia coli
thymidine phosphate
-
Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
2'-deoxyuridylate
-
Escherichia coli
0.0051
-
2'-deoxyuridylate in presence of 10 mM Mg2+ Lacticaseibacillus casei
0.0057
-
2'-deoxyuridylate
-
Enterococcus faecalis
0.0075
-
2'-deoxyuridylate
-
Gallus gallus
0.009
-
2'-deoxyuridylate
-
Bos taurus
0.014
-
2'-deoxyuridylate Ehrlich ascites carcinoma cells Mus musculus
0.018
-
2'-deoxyuridylate phage infected E. coli Escherichia phage T2
0.02
-
2'-deoxyuridylate less purified enzyme Bos taurus
0.03
-
2'-deoxyuridylate phage infected E. coli Enterobacteria phage T6
0.037
-
2'-deoxyuridylate Ehrlich ascites carcinoma cells Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP Gallus gallus
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Escherichia coli
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Bos taurus
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Enterococcus faecalis
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Escherichia phage T2
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Enterobacteria phage T6
-
dihydrofolate + dTMP
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
calf
-
Enterobacteria phage T6
-
-
-
Enterococcus faecalis
-
-
-
Escherichia coli
-
-
-
Escherichia phage T2
-
-
-
Gallus gallus
-
chicken
-
Lacticaseibacillus casei
-
-
-
Mus musculus
-
Ehrlich ascites carcinoma cells
-

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus
-
Bos taurus
Ehrlich ascites carcinoma cells Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Gallus gallus
-
thymus
-
Mus musculus
-
thymus thymus gland Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00035
-
-
Bos taurus
54
-
-
Enterococcus faecalis
150
-
-
Lacticaseibacillus casei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP
-
Gallus gallus dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Gallus gallus dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Mus musculus dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Mus musculus dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Bos taurus dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Bos taurus dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Enterococcus faecalis dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Enterococcus faecalis dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Lacticaseibacillus casei dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Lacticaseibacillus casei dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Escherichia phage T2 dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Escherichia phage T2 dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Enterobacteria phage T6 dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Enterobacteria phage T6 dihydrofolate + dTMP
-
r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
38
-
unstable above Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 6.8
-
Lacticaseibacillus casei
6.5 6.8 phosphate buffer Bos taurus
6.6
-
-
Bos taurus
7.5
-
-
Gallus gallus
7.5 8
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8
-
Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.024
-
dTMP
-
Enterococcus faecalis
0.059
-
dTMP
-
Bos taurus
0.14
-
dTMP
-
Gallus gallus