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Literature summary for 2.1.1.45 extracted from

  • Fridland, A.; Langenbach, R.J.; Heidelberger, C.
    Purification of thymidylate synthetase from Ehrlich ascites carcinoma cells (1971), J. Biol. Chem., 246, 7110-7114.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
beta-mercaptoethanol
-
Mus musculus
dithiothreitol
-
Escherichia coli
dithiothreitol
-
Homo sapiens
dithiothreitol thiols enhances enzyme activity Mus musculus

General Stability

General Stability Organism
enzyme is not inactivated by freezing and thawing Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
5-fluoro-2'-deoxyuridylic acid
-
Enterococcus faecalis
5-fluoro-2'-deoxyuridylic acid
-
Escherichia coli
5-fluoro-2'-deoxyuridylic acid
-
Homo sapiens
5-fluoro-2'-deoxyuridylic acid
-
Lacticaseibacillus casei
5-fluoro-2'-deoxyuridylic acid
-
Mus musculus
p-chloromercuribenzoate
-
Lacticaseibacillus casei
trifluoromethyldeoxyuridine 5'-monophosphate
-
Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0063
-
2'-deoxyuridylate
-
Mus musculus
0.043
-
5,10-methylenetetrahydrofolate
-
Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
-
Gallus gallus
67000
-
-
Lacticaseibacillus casei
67000
-
Ehrlich ascites carcinoma cells, gel filtration Mus musculus
70000
-
-
Mus musculus
70000
-
-
Escherichia coli
70000
-
-
Bos taurus
70000
-
-
Lacticaseibacillus casei
71000
-
-
Lacticaseibacillus casei
71000
-
Ehrlich ascites carcinoma cells, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP Gallus gallus
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Bos taurus
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Escherichia coli biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Homo sapiens biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Enterococcus faecalis biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
calf
-
Enterococcus faecalis
-
-
-
Escherichia coli
-
-
-
Gallus gallus
-
chicken
-
Homo sapiens
-
-
-
Lacticaseibacillus casei
-
-
-
Mus musculus
-
albino Swiss mice
-
Mus musculus
-
Ehrlich ascites carcinoma cells
-

Purification (Commentary)

Purification (Comment) Organism
Ehrlich ascites carcinoma cells Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Gallus gallus
-
thymus
-
Mus musculus
-
thymus
-
Homo sapiens
-
thymus
-
Bos taurus
-

Storage Stability

Storage Stability Organism
-20°C, sulfate-precipitated enzyme in 0.1 M potassium phosphate, 0.01 M dithiothreitol, pH 7.1can be kept at least 4 months with only 25% loss of activity Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP
-
Gallus gallus dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Gallus gallus dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Mus musculus dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Mus musculus dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Homo sapiens dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Bos taurus dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Bos taurus dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Enterococcus faecalis dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Lacticaseibacillus casei dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Lacticaseibacillus casei dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Escherichia coli dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Homo sapiens dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Enterococcus faecalis dihydrofolate + dTMP
-
?

Subunits

Subunits Comment Organism
monomer 1 * 71000, Ehrlich ascites carcinoma cells, SDS-PAGE Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
-
Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
Mus musculus