Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | - |
Mus musculus | |
dithiothreitol | - |
Escherichia coli | |
dithiothreitol | - |
Homo sapiens | |
dithiothreitol | thiols enhances enzyme activity | Mus musculus |
General Stability | Organism |
---|---|
enzyme is not inactivated by freezing and thawing | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluoro-2'-deoxyuridylic acid | - |
Enterococcus faecalis | |
5-fluoro-2'-deoxyuridylic acid | - |
Escherichia coli | |
5-fluoro-2'-deoxyuridylic acid | - |
Homo sapiens | |
5-fluoro-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei | |
5-fluoro-2'-deoxyuridylic acid | - |
Mus musculus | |
p-chloromercuribenzoate | - |
Lacticaseibacillus casei | |
trifluoromethyldeoxyuridine 5'-monophosphate | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0063 | - |
2'-deoxyuridylate | - |
Mus musculus | |
0.043 | - |
5,10-methylenetetrahydrofolate | - |
Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
- |
Gallus gallus |
67000 | - |
- |
Lacticaseibacillus casei |
67000 | - |
Ehrlich ascites carcinoma cells, gel filtration | Mus musculus |
70000 | - |
- |
Mus musculus |
70000 | - |
- |
Escherichia coli |
70000 | - |
- |
Bos taurus |
70000 | - |
- |
Lacticaseibacillus casei |
71000 | - |
- |
Lacticaseibacillus casei |
71000 | - |
Ehrlich ascites carcinoma cells, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Gallus gallus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Bos taurus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Enterococcus faecalis | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Enterococcus faecalis | - |
- |
- |
Escherichia coli | - |
- |
- |
Gallus gallus | - |
chicken | - |
Homo sapiens | - |
- |
- |
Lacticaseibacillus casei | - |
- |
- |
Mus musculus | - |
albino Swiss mice | - |
Mus musculus | - |
Ehrlich ascites carcinoma cells | - |
Purification (Comment) | Organism |
---|---|
Ehrlich ascites carcinoma cells | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Gallus gallus | - |
thymus | - |
Mus musculus | - |
thymus | - |
Homo sapiens | - |
thymus | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
-20°C, sulfate-precipitated enzyme in 0.1 M potassium phosphate, 0.01 M dithiothreitol, pH 7.1can be kept at least 4 months with only 25% loss of activity | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Gallus gallus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Gallus gallus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Enterococcus faecalis | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Homo sapiens | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Enterococcus faecalis | dihydrofolate + dTMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 71000, Ehrlich ascites carcinoma cells, SDS-PAGE | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
- |
Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Mus musculus |