Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Escherichia coli | |
dithiothreitol | enzyme activity is stimulated 2fold by 8-20 mM | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Homo sapiens |
medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Lacticaseibacillus casei |
medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Escherichia phage T2 |
General Stability | Organism |
---|---|
enzyme is stabilized by addition of 20-120 mM dithiothreitol + bovine serum albumin | Homo sapiens |
stable to protein denaturants and limited proteolysis | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * 33000, SDS-PAGE | Homo sapiens |
66000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Bos taurus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia phage T2 | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Escherichia coli | - |
- |
- |
Escherichia phage T2 | - |
- |
- |
Homo sapiens | - |
human | - |
Homo sapiens | - |
neoplastic leukemic CCRF-CEM cells | - |
Lacticaseibacillus casei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Bos taurus |
partially | Lacticaseibacillus casei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blast cell | - |
Homo sapiens | - |
thymus | - |
Homo sapiens | - |
thymus | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.8 | - |
- |
Homo sapiens |
3.8 | - |
- |
Escherichia phage T2 |
Storage Stability | Organism |
---|---|
-25°C enzyme retains activity for 3 months when stored in presence of thiols, without thiols enzyme loses all activity in 2 days | Homo sapiens |
4°C enzyme retains activity for 3 months when stored in presence of thiols, without thiols enzyme loses all activity in 2 days | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia phage T2 | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia phage T2 | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Homo sapiens | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Homo sapiens | dihydrofolate + dTMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
dimer | - |
Bos taurus |
dimer | - |
Lacticaseibacillus casei |
dimer | - |
Escherichia phage T2 |
dimer | 2 * 33000, SDS-PAGE | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.17 | - |
dUMP | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.1 | - |
Homo sapiens |