Information on EC 5.4.99.47 - parkeol synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
5.4.99.47
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RECOMMENDED NAME
GeneOntology No.
parkeol synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(3S)-2,3-epoxy-2,3-dihydrosqualene = parkeol
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Sesquiterpenoid and triterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(3S)-2,3-epoxy-2,3-dihydrosqualene mutase (cyclizing, parkeol-forming)
The enzyme from rice (Oryza sativa) produces parkeol as a single product [1].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3S)-2,3-oxidosqualene
achilleol A + protosta-12,24-dien-3beta-ol + lanosterol + parkeol
show the reaction diagram
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oxidosqualene-lanosterol cyclase mutant H234Y produces a product ratio of 14:26:51:9
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?
(3S)-2,3-oxidosqualene
cycloartenol + lanosterol + parkeol
show the reaction diagram
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the cycloartenol synthase 1 mutant I481V makes a relative product ratio of 52:26:22
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?
(3S)-2,3-oxidosqualene
lanosterol + parkeol
show the reaction diagram
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1:1 product ratio
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?
(3S)-2,3-oxidosqualene
parkeol
show the reaction diagram
(S)-2,3-oxidosqualene
parkeol + cycloartenol + lanosterol
show the reaction diagram
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parkeol is the main product of mutant N478H/V482I
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?
(S)-2,3-oxidosqualene
parkeol + lanosterol + lanost-24-ene-3beta,9alpha-diol
show the reaction diagram
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lanosterol-synthase mutant T384Y produces 11% parkeol, 79% lanosterol and 10% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene, lanosterol-synthase mutant T384Y/V4545L produces 60% parkeol, 20% lanosterol and 20% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene, lanosterol-synthase mutant T384Y/V454I produces 64% parkeol, 13% lanosterol and 23% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene
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?
oxidosqualene
lanosterol + parkeol
show the reaction diagram
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mutant H477N produces 88% lanosterol and 12% parkeol, mutant H477Q produces 73% parkeol, 22% lanosterol, and 5% DELTA7-lanosterol
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?
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae GIL77 cells; expressed in Saccharomyces cerevisiae strain GIL77
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H477N
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the mutant produces 88% lanosterol and 12% parkeol from oxidosqualene
H477Q
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the mutant produces 73% parkeol, 22% lanosterol, and 5% DELTA7-lanosterol from oxidosqualene
Q450H
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nonviable single mutant produces minor amount achilleol A as the sole product
Q450H/V454I
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mutant produces lanosterol, (13alphaH)-isomalabarica-14(26),17E,21-trien-3 beta-ol, protosta-16,24-dien-3beta-ol in a ratio of 75:16:9
T384Y
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mutant produces lanosterol, parkeol, 9beta-lanosta-7,24-dien-3 beta-ol, protosta-13(17),24-dien-3beta-ol and protosta-16,24-dien-3beta-ol in the ratio of 63:31.5:3.5:1:1 without compounds achilleol A and (13alphaH)-isomalabarica-14(26),17E,21-trien-3 beta-ol
T384Y/Q450H
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mutant produces lanosterol, parkeol, 9 beta -lanosta-7,24-dien-3 beta-ol in a ratio of 1:98:1
T384Y/Q450H/V454I
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mutant generates parkeol as the sole product, Vmax/Km 18fold decreased for 3S-2,3-oxidosqualene compared to wild-type
T384Y/V454I
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mutant produces lanosterol, parkeol, 9 beta -lanosta-7,24-dien-3 beta-ol in a ratio of 10:84:6
V454I
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mutant produces poor amounts of lanosterol and (13alphaH)-isomalabarica-14(26),17E,21-trien-3 beta-ol (ratio 90:10)