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Literature summary for 5.4.99.47 extracted from

  • Liu, Y.T.; Hu, T.C.; Chang, C.H.; Shie, W.S.; Wu, T.K.
    Protein engineering of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase into parkeol synthase (2012), Org. Lett., 14, 5222-5225.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
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Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
Q450H nonviable single mutant produces minor amount achilleol A as the sole product Saccharomyces cerevisiae
Q450H/V454I mutant produces lanosterol, (13alphaH)-isomalabarica-14(26),17E,21-trien-3 beta-ol, protosta-16,24-dien-3beta-ol in a ratio of 75:16:9 Saccharomyces cerevisiae
T384Y mutant produces lanosterol, parkeol, 9beta-lanosta-7,24-dien-3 beta-ol, protosta-13(17),24-dien-3beta-ol and protosta-16,24-dien-3beta-ol in the ratio of 63:31.5:3.5:1:1 without compounds achilleol A and (13alphaH)-isomalabarica-14(26),17E,21-trien-3 beta-ol Saccharomyces cerevisiae
T384Y/Q450H mutant produces lanosterol, parkeol, 9 beta -lanosta-7,24-dien-3 beta-ol in a ratio of 1:98:1 Saccharomyces cerevisiae
T384Y/Q450H/V454I mutant generates parkeol as the sole product, Vmax/Km 18fold decreased for 3S-2,3-oxidosqualene compared to wild-type Saccharomyces cerevisiae
T384Y/V454I mutant produces lanosterol, parkeol, 9 beta -lanosta-7,24-dien-3 beta-ol in a ratio of 10:84:6 Saccharomyces cerevisiae
V454I mutant produces poor amounts of lanosterol and (13alphaH)-isomalabarica-14(26),17E,21-trien-3 beta-ol (ratio 90:10) Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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Synonyms

Synonyms Comment Organism
ERG7
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Saccharomyces cerevisiae
Oxidosqualene-lanosterol cyclase
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Saccharomyces cerevisiae