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3-(2-benzothiazolylthio)-1-propanesulfonic acid
binds to the dimer interface of the enzyme and thereby abolishes its function with a high level of selectivity
bromohydroxyacetone phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
methylmethane thiosulfonate
inactivation by sulfhydroxylation
(1E,3E,6E,8E)-1,9-di(furan-2-yl)nona-1,3,6,8-tetraen-5-one
-
compound binds to the dimer interface and is unable to inactivate Trypanosoma brucei TIM or Homo sapiens TIM at concentrations higher than 100 microM. Compound also affects cruzipain
(1Z,2Z)-N,N'-dihydroxy-4-methylcyclohexa-3,5-diene-1,2-diimine
-
-
(2E)-2-[(4-methyl-5-oxido-1,2,5-oxadiazol-3-yl)methylidene]hydrazinecarbothioamide
-
-
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazinecarbothioamide
-
irreversible inhibitor
(2E)-2-[2-[(3-oxido-2,1,3-benzoxadiazol-5-yl)methoxy]benzylidene]-N-(prop-2-en-1-yl)hydrazinecarbothioamide
-
irreversible inhibitor
(2E)-N-(naphthalen-2-yl)-2-[(2E)-3-(5-nitrofuran-2-yl)prop-2-en-1-ylidene]hydrazinecarboxamide
-
irreversible inhibitor
(2E)-N-[2-(3,4-dimethoxyphenyl)ethyl]-2-[(5-nitrofuran-2-yl)methylidene]hydrazinecarboxamide
-
irreversible inhibitor
(2E,5E)-2,5-bis[(2E)-3-(thiophen-2-yl)prop-2-en-1-ylidene]cyclopentan-1-one
-
compound is unable to inactivate Trypanosoma brucei TIM or Homo sapiens TIM at concentrations higher than 100 microM. Compound also affects cruzipain
(2E,6E)-2,6-bis[(2E)-3-(furan-2-yl)prop-2-en-1-ylidene]cyclohexan-1-one
-
compound binds to the dimer interface and is unable to inactivate Trypanosoma brucei TIM or Homo sapiens TIM at concentrations higher than 100 microM. Compound also affects cruzipain
(4-methyl-1,2,3-thiadiazol-5-yl)methanol
-
29% inhibition at 0.4 mM
1,2,6-thiadiazine
-
irreversible inhibitor
2,2'-methylenebis(1,3-benzothiazole)
-
40% inactivation at 0.05 mM, irreversible
2,6-dibenzyl-4-[(5-nitrothiophen-2-yl)methylidene]-1,2,6-thiadiazinane-3,5-dione 1,1-dioxide
-
irreversible inhibitor
2-phenyl-4H-chromen-4-one
-
-
2-[(1E)-2-nitroprop-1-en-1-yl]thiophene
-
-
2-[(3-aminophenyl)disulfanyl]aniline
-
-
3,5-diphenyl-1,2,4-thiadiazole
-
74% inhibition at 0.4 mM
3-(4-methylphenyl)-5-[(4-methylphenyl)sulfonyl]-1,2,4-thiadiazole
-
-
3-nitrobiphenyl-4-amine
-
-
4-(4-nitrobenzylidene)-2,6-bis(2-phenylethyl)-1,2,6-thiadiazinane-3,5-dione 1,1-dioxide
-
irreversible inhibitor, 85% inhibition at 0.4 mM
4-[(5-nitrofuran-2-yl)methylidene]-4H-1,2,6-thiadiazine-3,5-diamine 1,1-dioxide
-
irreversible inhibitor
4-[(5-nitrothiophen-2-yl)methylidene]-2,6-bis(2-phenylethyl)-1,2,6-thiadiazinane-3,5-dione 1,1-dioxide
-
irreversible inhibitor
5,5'-dithiobis(2-nitrobenzoate)
-
-
5-[(1E)-2-nitroprop-1-en-1-yl]-1,3-benzodioxole
-
-
6,6'-bi-1,3-benzothiazole-2,2'-diamine
6-[(E)-2-(5-nitrothiophen-2-yl)ethenyl]-2,1,3-benzoxadiazole 1-oxide
-
irreversible inhibitor
6-[(E)-2-[(4-fluorophenyl)sulfanyl]ethenyl]-2,1,3-benzoxadiazole 1-oxide
-
irreversible inhibitor
8-bromo-5,10-dioxidophenazin-2-yl chloroacetate
-
50% inhibition at 0.4 mM
butylbrevifolin carboxylate
-
-
ethyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)-1,2,4-thiadiazole-5-carboxylate
-
-
ethyl 3-phenyl-1,2,4-thiadiazole-5-carboxylate
-
-
ethylbrevifolin carboxylate
-
-
methyl methanethiosulfonate
-
the sensitivity of enzyme from Trypanosoma cruzi is about 40times higher than that of Trypanosoma brucei and 200times higher than that of Leishmania mexicana
methylbrevifolin carboxylate
-
molecular docking simulations and enzyme binding structure, and inhibition kinetics, overview
methylmethane thiosulfonate
N-[(2-oxido-4-phenyl-1,2,5-oxadiazol-3-yl)methyl]naphthalen-1-amine
-
72% inhibition at 0.4 mM
N-[(4-methyl-5-oxido-1,2,5-oxadiazol-3-yl)methyl]naphthalen-1-amine
-
41% inhibition at 0.4 mM
phenazine 5,9-dioxide
-
84% inhibition at 0.4 mM
additional information
-
brevifolin carboxylate derivatives isolated from Geranium bellum selectively inactivate the enzyme partially, no inhibition by methyl tri-O-methylbrevifolin carboxylate
-
6,6'-bi-1,3-benzothiazole-2,2'-diamine
-
-
6,6'-bi-1,3-benzothiazole-2,2'-diamine
-
91% inactivation at 0.05 mM, irreversible. Not inhibitory on human, yeast, chicken, Plasmodium falciparum, and Entamoeba histolytica enzyme
methylmethane thiosulfonate
-
almost complete inhibition at 0.1 mM
methylmethane thiosulfonate
-
modification at C15 in the dimer interface, inducing abolition of catalysis and structural changes. Susceptibility of Trypanosoma cruzi enzyme to modification of C15 is nearly 100fold higher than susceptibility of C15 of Trypanosoma brucei
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0.003
(1E,3E,6E,8E)-1,9-di(furan-2-yl)nona-1,3,6,8-tetraen-5-one
Trypanosoma cruzi
-
pH 7.4, 25°C
0.1
(1Z,2Z)-N,N'-dihydroxy-4-methylcyclohexa-3,5-diene-1,2-diimine
Trypanosoma cruzi
-
IC50 about 0.1 mM, in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.1
(2E)-2-[(4-methyl-5-oxido-1,2,5-oxadiazol-3-yl)methylidene]hydrazinecarbothioamide
Trypanosoma cruzi
-
IC50 about 0.1 mM, in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.1
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazinecarbothioamide
Trypanosoma cruzi
-
IC50 about 0.1 mM, in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.1
(2E)-2-[2-[(3-oxido-2,1,3-benzoxadiazol-5-yl)methoxy]benzylidene]-N-(prop-2-en-1-yl)hydrazinecarbothioamide
Trypanosoma cruzi
-
IC50 about 0.1 mM, in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.1
(2E)-N-(naphthalen-2-yl)-2-[(2E)-3-(5-nitrofuran-2-yl)prop-2-en-1-ylidene]hydrazinecarboxamide
Trypanosoma cruzi
-
IC50 about 0.1 mM, in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.03 - 0.1
(2E)-N-[2-(3,4-dimethoxyphenyl)ethyl]-2-[(5-nitrofuran-2-yl)methylidene]hydrazinecarboxamide
Trypanosoma cruzi
-
in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.0047
(2E,5E)-2,5-bis[(2E)-3-(thiophen-2-yl)prop-2-en-1-ylidene]cyclopentan-1-one
Trypanosoma cruzi
-
pH 7.4, 25°C
0.000086
(2E,6E)-2,6-bis[(2E)-3-(furan-2-yl)prop-2-en-1-ylidene]cyclohexan-1-one
Trypanosoma cruzi
-
pH 7.4, 25°C
0.0035
1,2,4-thiadiazole
Trypanosoma cruzi
-
in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.013
1,2,6-thiadiazine
Trypanosoma cruzi
-
in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.01
1,3,4-oxathiazole
Trypanosoma cruzi
-
in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.03 - 0.1
2,6-dibenzyl-4-[(5-nitrothiophen-2-yl)methylidene]-1,2,6-thiadiazinane-3,5-dione 1,1-dioxide
Trypanosoma cruzi
-
in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.1
2-phenyl-4H-chromen-4-one
Trypanosoma cruzi
-
IC50 about 0.1 mM, in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.1
2-[(1E)-2-nitroprop-1-en-1-yl]thiophene
Trypanosoma cruzi
-
IC50 about 0.1 mM, in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.1
3,5-diphenyl-1,2,4-thiadiazole
Trypanosoma cruzi
-
IC50 about 0.1 mM, in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.1
3-(4-methylphenyl)-5-[(4-methylphenyl)sulfonyl]-1,2,4-thiadiazole
Trypanosoma cruzi
-
IC50 about 0.1 mM, in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.1
3-nitrobiphenyl-4-amine
Trypanosoma cruzi
-
IC50 about 0.1 mM, in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.1
4-(4-nitrobenzylidene)-2,6-bis(2-phenylethyl)-1,2,6-thiadiazinane-3,5-dione 1,1-dioxide
Trypanosoma cruzi
-
IC50 about 0.1 mM, in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.02
4-[(5-nitrofuran-2-yl)methylidene]-4H-1,2,6-thiadiazine-3,5-diamine 1,1-dioxide
Trypanosoma cruzi
-
in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.1
4-[(5-nitrothiophen-2-yl)methylidene]-2,6-bis(2-phenylethyl)-1,2,6-thiadiazinane-3,5-dione 1,1-dioxide
Trypanosoma cruzi
-
IC50 about 0.1 mM, in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.1
5-[(1E)-2-nitroprop-1-en-1-yl]-1,3-benzodioxole
Trypanosoma cruzi
-
IC50 about 0.1 mM, in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.03 - 0.1
6-[(E)-2-(5-nitrothiophen-2-yl)ethenyl]-2,1,3-benzoxadiazole 1-oxide
Trypanosoma cruzi
-
in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.1
6-[(E)-2-[(4-fluorophenyl)sulfanyl]ethenyl]-2,1,3-benzoxadiazole 1-oxide
Trypanosoma cruzi
-
IC50 about 0.1 mM, in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.4
8-bromo-5,10-dioxidophenazin-2-yl chloroacetate
Trypanosoma cruzi
-
in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.014
butylbrevifolin carboxylate
Trypanosoma cruzi
-
-
0.1
ethyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)-1,2,4-thiadiazole-5-carboxylate
Trypanosoma cruzi
-
IC50 about 0.1 mM, in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.03 - 0.1
ethyl 3-phenyl-1,2,4-thiadiazole-5-carboxylate
Trypanosoma cruzi
-
in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.008
ethylbrevifolin carboxylate
Trypanosoma cruzi
-
-
0.0066
methylbrevifolin carboxylate
Trypanosoma cruzi
-
-
0.1
N-[(2-oxido-4-phenyl-1,2,5-oxadiazol-3-yl)methyl]naphthalen-1-amine
Trypanosoma cruzi
-
IC50 about 0.1 mM, in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
0.026
phenazine 5,9-dioxide
Trypanosoma cruzi
-
in 100 mM triethanolamine, 10 mM EDTA, pH 7.4 and 10% of dimethyl sulfoxide, at 36°C
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E26D/T27L/L28F/A30S/L100A/Q115A
mutant construcuted to produce a TIM with the inactivation susceptibility pattern of Trypanosoma brucei brucei enzyme, shows a similar resistance pattern to the inactivation with methylmethane thiosulfonate as wild-type Trypanosoma brucei brucei TIM
E26D/T27L/L28F/A30S/T32S/L100A/Q115A
mutant construcuted to produce a TIM with the inactivation susceptibility pattern of Trypanosoma brucei brucei enzyme, shows a similar resistance pattern to the inactivation with methylmethane thiosulfonate as wild-type Trypanosoma brucei brucei TIM
E26D/T27L/L28F/L100A/Q115A
mutant construcuted to produce a TIM with the inactivation susceptibility pattern of Trypanosoma brucei brucei enzyme, shows a similar resistance pattern to the inactivation with methylmethane thiosulfonate as wild-type Trypanosoma brucei brucei TIM
L28F/L100A/Q115A
mutant construcuted to produce a TIM with the inactivation susceptibility pattern of Trypanosoma brucei brucei enzyme. Protein is more resistant to the inactivation with methylmethane thiosulfonate as wild-type
C15A
-
dimer formation of mutant protein with intact Trypanosoma brucei monomer, maximal inhibition of catalysis by mutation is 60%. Dimer formation with catalytically inert Trypanosoma brucei mutant E168D causes a drop in activity by 50%
additional information
analysis of chimera between Trypanosoma cruzi and Trypanosoma brucei brucei enzymes. The (betaalpha)2 motif of Trypanosoma cruzi TIM inserted into Trypanosoma brucei brucei TIM increases the kinetic stability. The presence of the (betaalpha)2 motif of Trypanosoma brucei brucei TIM inserted into Trypanosoma cruzi TIM gives a chimerical protein difficult to purify in soluble form and with a significantly reduces kinetic stability
additional information
-
analysis of chimera between Trypanosoma cruzi and Trypanosoma brucei brucei enzymes. The (betaalpha)2 motif of Trypanosoma cruzi TIM inserted into Trypanosoma brucei brucei TIM increases the kinetic stability. The presence of the (betaalpha)2 motif of Trypanosoma brucei brucei TIM inserted into Trypanosoma cruzi TIM gives a chimerical protein difficult to purify in soluble form and with a significantly reduces kinetic stability
additional information
construction of mutants for interconversion of the behavior of the enzymes from Trypanosoma brucei brucei and Trypanosoma cruzi. To make Trypanosoma cruzi TIM reactivate with a Trypanosoma brucei brucei TIM-like behaviour you need to mutate the following amino acids: 18, 19, 20, 22, 23 and 26, and R2: 43, 46, 48, 49, 53, 56 and 57. To make a Trypanosoma brucei brucei TIM reactivate with a Trypanosoma cruzi TIM-like behaviour you need to mutate amino acids 18, 23, 26, 32, 33 and 34, and 43, 46, 48, 49, 53, 56 and 57
additional information
-
construction of mutants for interconversion of the behavior of the enzymes from Trypanosoma brucei brucei and Trypanosoma cruzi. To make Trypanosoma cruzi TIM reactivate with a Trypanosoma brucei brucei TIM-like behaviour you need to mutate the following amino acids: 18, 19, 20, 22, 23 and 26, and R2: 43, 46, 48, 49, 53, 56 and 57. To make a Trypanosoma brucei brucei TIM reactivate with a Trypanosoma cruzi TIM-like behaviour you need to mutate amino acids 18, 23, 26, 32, 33 and 34, and 43, 46, 48, 49, 53, 56 and 57
additional information
-
hybrids of Trypanosoma cruzi enzyme carrying residues of the dimer interface from Trypanomsoma brucei and vice versa, and hybrids with one monomer in the enzyme dimer from Trypanosoma cruzi, and the second monomer from Trypanosoma brucei. Solvent exposure of the interfacial C15 depends predominantly on the characteristics of the adjoining monomer. Half of the activity of each monomer depends on the integrity of each of the two C15-loop3 portions of the interface
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Ostoa-Saloma, P.; Garza-Ramos, G.; Ramirez, J.; Becker, I.; Berzunza, M.; Landa, A.; Gomez-Puyou, A.; Tuena de Gomez-Puyou, M.; Perez-Montfort, R.
Cloning, expression, purification and characterization of triosephosphate isomerase from Trypanosoma cruzi
Eur. J. Biochem.
244
700-705
1997
Leishmania mexicana, Trypanosoma brucei, Trypanosoma cruzi
brenda
Gao, X.G.; Maldonado, E.; Perez-Montfort, R.; Garza-Ramos, G.; de Gomez-Puyou, M.T.; Gomez-Puyou, A.; Rodriguez-Romero, A.
Crystal structure of triosephosphate isomerase from Trypanosoma cruzi in hexane
Proc. Natl. Acad. Sci. USA
96
10062-10067
1999
Trypanosoma cruzi (P52270), Trypanosoma cruzi
brenda
Chanez-Cardenas, M.E.; Perez-Hernandez, G.; Sanchez-Rebollar, B.G.; Costas, M.; Vazquez-Contreras, E.
Reversible equilibrium unfolding of triosephosphate isomerase from Trypanosoma cruzi in guanidinium hydrochloride involves stable dimeric and monomeric intermediates
Biochemistry
44
10883-10892
2005
Trypanosoma cruzi
brenda
Tellez-Valencia, A.; Olivares-Illana, V.; Hernandez-Santoyo, A.; Perez-Montfort, R.; Costas, M.; Rodriguez-Romero, A.; Lopez-Calahorra, F.; Tuena De Gomez-Puyou, M.; Gomez-Puyou, A.
Inactivation of triosephosphate isomerase from Trypanosoma cruzi by an agent that perturbs its dimer interface
J. Mol. Biol.
341
1355-1365
2004
Trypanosoma cruzi (P52270), Trypanosoma cruzi
brenda
Olivares-Illana, V.; Perez-Montfort, R.; Lopez-Calahorra, F.; Costas, M.; Rodriguez-Romero, A.; Tuena de Gomez-Puyou, M.; Gomez Puyou, A.
Structural differences in triosephosphate isomerase from different species and discovery of a multitrypanosomatid inhibitor
Biochemistry
45
2556-2560
2006
Leishmania mexicana, Trypanosoma brucei, Trypanosoma cruzi
brenda
Zomosa-Signoret, V.; Aguirre-Lopez, B.; Hernandez-Alcantara, G.; Perez-Montfort, R.; Tuena de Gomez-Puyou, M.; Gomez-Puyou, A.
Crosstalk between the subunits of the homodimeric enzyme triosephosphate isomerase
Proteins Struct. Funct. Bioinform.
67
75-83
2007
Trypanosoma brucei, Trypanosoma cruzi
brenda
Zarate-Perez, F.; Chanez-Cardenas, M.E.; Arreola, R.; Torres-Larios, A.; Vazquez-Contreras, E.
Different catalytic properties of two highly homologous triosephosphate isomerase monomers
Biochem. Biophys. Res. Commun.
382
626-630
2009
Trypanosoma cruzi (P52270)
brenda
Gayosso-De-Lucio, J.; Torres-Valencia, M.; Rojo-Dominguez, A.; Najera-Pena, H.; Aguirre-Lopez, B.; Salas-Pacheco, J.; Avitia-Dominguez, C.; Tellez-Valencia, A.
Selective inactivation of triosephosphate isomerase from Trypanosoma cruzi by brevifolin carboxylate derivatives isolated from Geranium bellum Rose
Bioorg. Med. Chem. Lett.
19
5936-5939
2009
Trypanosoma cruzi
brenda
Wierenga, R.K.; Kapetaniou, E.G.; Venkatesan, R.
Triosephosphate isomerase: a highly evolved biocatalyst
Cell. Mol. Life Sci.
67
3961-3982
2010
Entamoeba histolytica (O02611), Oryctolagus cuniculus (P00939), Gallus gallus (P00940), Saccharomyces cerevisiae (P00942), Geobacillus stearothermophilus (P00943), Trypanosoma brucei brucei (P04789), Escherichia coli (P0A858), Giardia intestinalis (P36186), Thermotoga maritima (P36204), Leishmania mexicana (P48499), Moritella marina (P50921), Trypanosoma cruzi (P52270), Helicobacter pylori (P56076), Homo sapiens (P60174), Pyrococcus woesei (P62003), Mycobacterium tuberculosis (P9WG43), Plasmodium falciparum (Q07412), Caenorhabditis elegans (Q10657), Methanocaldococcus jannaschii (Q58923), Bartonella henselae (Q8L1Z5), Tenebrio molitor (Q8MPF2), Thermoproteus tenax (Q8NKN9), Mycobacterium tuberculosis H37Rv (P9WG43)
brenda
Alvarez, G.; Aguirre-Lopez, B.; Varela, J.; Cabrera, M.; Merlino, A.; Lopez, G.V.; Lavaggi, M.L.; Porcal, W.; Di Maio, R.; Gonzalez, M.; Cerecetto, H.; Cabrera, N.; Perez-Montfort, R.; de Gomez-Puyou, M.T.; Gomez-Puyou, A.
Massive screening yields novel and selective Trypanosoma cruzi triosephosphate isomerase dimer-interface-irreversible inhibitors with anti-trypanosomal activity
Eur. J. Med. Chem.
45
5767-5772
2010
Homo sapiens, Trypanosoma cruzi, Trypanosoma cruzi T2
brenda
Garcia-Torres, I.; Cabrera, N.; Torres-Larios, A.; Rodriguez-Bolanos, M.; Diaz-Mazariegos, S.; Gomez-Puyou, A.; Perez-Montfort, R.
Identification of amino acids that account for long-range interactions in two triosephosphate isomerases from pathogenic trypanosomes
PLoS ONE
6
e18791
2011
Trypanosoma brucei, Trypanosoma cruzi
brenda
Aguilera, E.; Varela, J.; Birriel, E.; Serna, E.; Torres, S.; Yaluff, G.; de Bilbao, N.; Aguirre-Lopez, B.; Cabrera, N.; Diaz Mazariegos, S.; de Gomez-Puyou, M.; Gomez-Puyou, A.; Perez-Montfort, R.; Minini, L.; Merlino, A.; Cerecetto, H.; Gonzalez, M.; Alvarez, G.
Potent and selective inhibitors of Trypanosoma cruzi triosephosphate isomerase with concomitant inhibition of cruzipain Inhibition of parasite growth through multitarget activity
ChemMedChem
17
1328-1338
2016
Trypanosoma cruzi, Trypanosoma cruzi Tulahuen 2
brenda
Rodriguez-Bolanos, M.; Cabrera, N.; Perez-Montfort, R.
Identification of the critical residues responsible for differential reactivation of the triosephosphate isomerases of two trypanosomes
Open biology
6
160161
2016
Trypanosoma brucei brucei (P04789), Trypanosoma cruzi (P52270), Trypanosoma cruzi
brenda
Diaz-Mazariegos, S.; Cabrera, N.; Perez-Montfort, R.
Three unrelated and unexpected amino acids determine the susceptibility of the interface cysteine to a sulfhydryl reagent in the triosephosphate isomerases of two trypanosomes
PLoS ONE
13
e0189525
2018
Trypanosoma brucei brucei (P04789), Trypanosoma cruzi (P52270)
brenda
Guzman-Luna, V.; Quezada, A.G.; Diaz-Salazar, A.J.; Cabrera, N.; Perez-Montfort, R.; Costas, M.
The effect of specific proline residues on the kinetic stability of the triosephosphate isomerases of two trypanosomes
Proteins
85
571-579
2017
Trypanosoma brucei brucei (P04789), Trypanosoma cruzi (P52270), Trypanosoma cruzi
brenda