Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli (DE3)pLysS cells | Trypanosoma brucei |
expressed in Escherichia coli (DE3)pLysS cells | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4,4'-Dithiopyridine | - |
Trypanosoma brucei | |
4,4'-Dithiopyridine | - |
Trypanosoma cruzi | |
5,5'-dithiobis(2-nitrobenzoate) | - |
Trypanosoma brucei | |
5,5'-dithiobis(2-nitrobenzoate) | - |
Trypanosoma cruzi | |
methylmethane thiosulfonate | 60% residual activity at 0.1 mM | Trypanosoma brucei | |
methylmethane thiosulfonate | almost complete inhibition at 0.1 mM | Trypanosoma cruzi | |
N-ethylmaleimide | - |
Trypanosoma brucei | |
N-ethylmaleimide | - |
Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
D-glyceraldehyde 3-phosphate | in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C | Trypanosoma cruzi | |
0.45 | - |
D-glyceraldehyde 3-phosphate | in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | Trypanosoma brucei | - |
Glycerone phosphate | - |
r | |
D-Glyceraldehyde 3-phosphate | Trypanosoma cruzi | - |
Glycerone phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Trypanosoma cruzi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Toyopearl column chromatography and SP-Sepharose column chromatography | Trypanosoma brucei |
ammonium sulfate precipitation, Toyopearl column chromatography and SP-Sepharose column chromatography | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | - |
Trypanosoma brucei | Glycerone phosphate | - |
r | |
D-Glyceraldehyde 3-phosphate | - |
Trypanosoma cruzi | Glycerone phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TIM | - |
Trypanosoma brucei |
TIM | - |
Trypanosoma cruzi |
Triosephosphate isomerase | - |
Trypanosoma brucei |
Triosephosphate isomerase | - |
Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4500 | - |
D-glyceraldehyde 3-phosphate | in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C | Trypanosoma cruzi | |
5167 | - |
D-glyceraldehyde 3-phosphate | in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C | Trypanosoma brucei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10500 | - |
D-glyceraldehyde 3-phosphate | in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C | Trypanosoma cruzi | |
11500 | - |
D-glyceraldehyde 3-phosphate | in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C | Trypanosoma brucei |