Information on EC 3.5.3.7 - guanidinobutyrase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.5.3.7
-
RECOMMENDED NAME
GeneOntology No.
guanidinobutyrase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-guanidinobutanoate + H2O = 4-aminobutanoate + urea
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of C-N bond
hydrolysis of linear amidines
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arginine and proline metabolism
-
-
L-arginine degradation IX (arginine:pyruvate transaminase pathway)
-
-
L-arginine degradation VIII (arginine oxidase pathway)
-
-
L-arginine degradation X (arginine monooxygenase pathway)
-
-
L-arginine degradation XII
-
-
Metabolic pathways
-
-
arginine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
4-guanidinobutanoate amidinohydrolase
Requires Mn2+. Also acts, very slowly, on 5-guanidinopentanoate and 6-guanidinohexanoate.
CAS REGISTRY NUMBER
COMMENTARY hide
37289-14-8
-
9013-69-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
axolotl, non-metamorphosed
-
-
Manually annotated by BRENDA team
IFO 12137 (ATCC 8010)
-
-
Manually annotated by BRENDA team
strain KUJ8602
SwissProt
Manually annotated by BRENDA team
strain KUJ8602
SwissProt
Manually annotated by BRENDA team
IFO 12073 (ATCC 11822)
-
-
Manually annotated by BRENDA team
Brevibacterium lipolyticum
IFO 12678
-
-
Manually annotated by BRENDA team
IFO 12072 (ATCC 6872)
-
-
Manually annotated by BRENDA team
IFO 12144
-
-
Manually annotated by BRENDA team
Crotalus sp.
rattlesnake
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
chicken
-
-
Manually annotated by BRENDA team
Gopherus sp.
turtle
-
-
Manually annotated by BRENDA team
snail
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Kinosternon sp.
turtle
-
-
Manually annotated by BRENDA team
no activity in Kluyveromyces lactis strain GG1632
-
-
-
Manually annotated by BRENDA team
no activity in Neurospora crassa
74A
-
-
Manually annotated by BRENDA team
no activity in Neurospora crassa 74A
74A
-
-
Manually annotated by BRENDA team
Pseudemys sp.
turtle
-
-
Manually annotated by BRENDA team
P2 (ATCC 25571)
-
-
Manually annotated by BRENDA team
frog
-
-
Manually annotated by BRENDA team
teleostan fresh-water tench, autochtonous fish, L.
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-guanidinopropinonate + H2O
3-aminopropanoate + urea
show the reaction diagram
-
-
-
-
?
3-guanidinopropionate + H2O
3-aminopropanoate + urea
show the reaction diagram
-
-
-
?
3-guanidopropionate + H2O
?
show the reaction diagram
-
-
-
-
?
4-guanidinobutanoate + H2O
4-aminobutanoate + urea
show the reaction diagram
4-Guanidinobutyrate + H2O
4-Aminobutyrate + urea
show the reaction diagram
5-guanidinovalerate + H2O
5-aminopentanoate + urea
show the reaction diagram
6-guanidinocaproate + H2O
6-aminohexanoate + urea
show the reaction diagram
arginine + H2O
2,5-diaminopentanoate + urea
show the reaction diagram
D-arginine + H2O
D-ornithine + urea
show the reaction diagram
-
-
-
?
L-arginine + H2O
L-ornithine + urea
show the reaction diagram
-
-
-
?
L-canavanine + H2O
L-2-amino-4-(aminooxy)butanoic acid + urea
show the reaction diagram
taurocyamine + H2O
taurine + urea
show the reaction diagram
-
strain U2-3-3 is a taurocyamine utilizing mutant
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-guanidinobutanoate + H2O
4-aminobutanoate + urea
show the reaction diagram
4-Guanidinobutyrate + H2O
4-Aminobutyrate + urea
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
can substitute for Zn2+
Ni2+
-
slight activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
inactivation due to removal of bound Zn2+, reversible by Zn2+ and Co2+, and partially by Mn2+
2-mercaptoethanol
-
-
2-mercaptopropionate
-
-
2-nitrobenzoic acid
-
slight inhibition
3-mercaptoethylamine
-
-
3-Mercaptopropionate
-
-
4-aminobutyrate
-
-
5-Aminovalerate
6-aminocaproate
-
-
Mercaptoacetate
strong mixed-type inhibitor
mercaptoethylamine
-
-
Mn2+
-
5 mM complete inhibition
n-Butyrate
N-ethylmaleimide
-
slight inhibition
n-valerate
-
-
-
o-phenanthroline
-
-
p-chloromercuribenzoate
-
reactivation by incubation with 2-mercaptoethanol
propionate
-
competitive inhibition
trans-crotonate
-
-
Zn2+
-
92% inhibition
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-Amino-1-propanol
-
5 mM, relative activity 113%
3-guanidinopropionate
-
induced by
DL-lactate
-
5 mM, relative activity 106%
fumarate
-
5 mM, relative activity 101%
Guanidinoacetate
-
5 mM, relative activity 104%
L-arginine
Maleate
-
5 mM, relative activity 104%
n-butylamine
-
5 mM, relative activity 118%
n-Propylamine
-
5 mM, relative activity 121%
succinate
-
5 mM, relative activity 103%
Taurocyamine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.9 - 49
4-Guanidinobutyrate
45 - 230
4-guanidinobutyric acid
450
4-guanidopropionic acid
-
-
206
5-Guanidinovalerate
-
at pH 10
163
6-Guanidinocaproate
-
at pH 10
71 - 200
L-arginine
1670
L-canvanine
-
-
-
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
94.7 - 1012
4-Guanidinobutyrate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.71
purified enzyme
64
-
EDTA treated, 0.2 mM Co(NO3)2
69
-
EDTA treated
79
-
EDTA treated, 0.2 mM MnSO4
135.7
-
purified enzyme
158
-
EDTA treated, 0.02 mM MnSO4
198
-
EDTA treated, 0.04 mM MnSO4
209
-
EDTA treated, 0.12 mM MnSO4
290
-
EDTA treated, 0.2 mM MnSO4
303
-
purified enzyme
910
-
PAO1, purified enzyme
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 11
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
-
4 * 34000, gel filtration, SDS-PAGE
34700
-
monomer, calculated from amino acid sequence
36000
-
6 * 36000, SDS-PAGE
40000
6 * 40000, SDS-PAGE
45000
-
4 * 45000, SDS-PAGE
65000
-
2 * 65000, room temperature, SDS-PAGE, compactly associated subunits
140000
-
gel filtration
170000
-
-
178000
180000 - 186000
185000
-
disc gel electrophoresis
190000
194000
-
gel filtration
210000
-
gel filtration, undenatured enzyme
232000
gel filtration
252000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 65000, room temperature, SDS-PAGE, compactly associated subunits
hexamer
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structures of Pseudomonas aeruginosa guanidinobutyrase (GbuA) and guanidinopropionase (GpuA) are determined. Although GbuA and GpuA share a common structural fold of the typical ureohydrolase superfamily, they exhibit significant variations in two active site loops
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.5
-
most stable at, at pH higher than 9.0 or lower than 6.5 potassium phosphate 50 mM and sodium chloride 0.3 M yields a stabilizing effect at pH 7.5-10.0
209476
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
no variation up to, higher temperatures have negative effect
50
-
residual activity after heating for 60 min 50% of the original activity
70
-
rather heat stable, no detectable loss of activity up to
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
relatively unstable in absence of Mn2+
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C stable to storage for at least 1 month
-
4°C, quite stable, retains basically the same activity several weeks, temperatures below 0°C have negative effects
-
4°C, unstable on storage, activity lost after 48 h, partially restored by incubation with Mn2+
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli
expressed in Saccharomyces cerevisiae
expression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
M161Y
-
mutant showed no detectable enzymatic activity against guanidium compounds
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