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Literature summary for 3.5.3.7 extracted from

  • Yorifuji, T.; Shimizu, E.; Hirata, H.; Imada, K.; Katsumi, T.; Sawamura, S.
    Guanidinobutyrase for L-arginine degradation in Brevibacterium helvolum (1992), Biosci. Biotechnol. Biochem., 56, 773-777.
    View publication on PubMed
Search for more literature for 3.5.3.7

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Brevibacterium helvolum
2-mercaptopropionate
-
 Brevibacterium helvolum
3-mercaptoethylamine
-
 Brevibacterium helvolum
3-Mercaptopropionate
-
 Brevibacterium helvolum
5-Aminovalerate
-
 Brevibacterium helvolum
6-aminocaproate
-
 Brevibacterium helvolum
agmatine
-
 Brevibacterium helvolum
mercaptoethylamine
-
 Brevibacterium helvolum
n-Butyrate
-
 Brevibacterium helvolum
n-valerate
-
 Brevibacterium helvolum
o-phenanthroline
-
 Brevibacterium helvolum
thioglycolate
-
 Brevibacterium helvolum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9
-
 4-Guanidinobutyrate
-
 Brevibacterium helvolum
71
-
 L-arginine
-
 Brevibacterium helvolum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ firmly bound Brevibacterium helvolum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 4 * 45000, SDS-PAGE Brevibacterium helvolum
65000
-
 2 * 65000, room temperature, SDS-PAGE, compactly associated subunits Brevibacterium helvolum
190000
-
 gel filtration Brevibacterium helvolum
194000
-
 gel filtration Brevibacterium helvolum
210000
-
 gel filtration, undenatured enzyme Brevibacterium helvolum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-Guanidinobutyrate + H2O Pseudomonas fluorescens
-
 4-Aminobutyrate + urea
-
 ?
4-Guanidinobutyrate + H2O Pimelobacter simplex
-
 4-Aminobutyrate + urea
-
 ?
4-Guanidinobutyrate + H2O Brevibacterium linens
-
 4-Aminobutyrate + urea
-
 ?
4-Guanidinobutyrate + H2O Arthrobacter globiformis
-
 4-Aminobutyrate + urea
-
 ?
4-Guanidinobutyrate + H2O Streptomyces griseus
-
 4-Aminobutyrate + urea
-
 ?
4-Guanidinobutyrate + H2O Brevibacterium helvolum
-
 4-Aminobutyrate + urea
-
 ?
4-Guanidinobutyrate + H2O Corynebacterium ammoniagenes
-
 4-Aminobutyrate + urea
-
 ?
4-Guanidinobutyrate + H2O Brevibacterium lipolyticum
-
 4-Aminobutyrate + urea
-
 ?
4-Guanidinobutyrate + H2O Corynebacterium stationis
-
 4-Aminobutyrate + urea
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
 IFO 12137 (ATCC 8010)
-
Brevibacterium helvolum
-
 IFO 12073 (ATCC 11822)
-
Brevibacterium linens
-
 IFO 12141
-
Brevibacterium lipolyticum
-
 IFO 12678
-
Corynebacterium ammoniagenes
-
 IFO 12072 (ATCC 6872)
-
Corynebacterium stationis
-
 IFO 12144
-
Pimelobacter simplex
-
 IFO 12069 (ATCC 6946)
-
Pseudomonas fluorescens
-
-
-
Streptomyces griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brevibacterium helvolum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
83.8
-
-
 Brevibacterium helvolum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-Guanidinobutyrate + H2O
-
 Pseudomonas fluorescens 4-Aminobutyrate + urea
-
 ?
4-Guanidinobutyrate + H2O
-
 Pimelobacter simplex 4-Aminobutyrate + urea
-
 ?
4-Guanidinobutyrate + H2O
-
 Brevibacterium linens 4-Aminobutyrate + urea
-
 ?
4-Guanidinobutyrate + H2O
-
 Arthrobacter globiformis 4-Aminobutyrate + urea
-
 ?
4-Guanidinobutyrate + H2O
-
 Streptomyces griseus 4-Aminobutyrate + urea
-
 ?
4-Guanidinobutyrate + H2O
-
 Brevibacterium helvolum 4-Aminobutyrate + urea
-
 ?
4-Guanidinobutyrate + H2O
-
 Corynebacterium ammoniagenes 4-Aminobutyrate + urea
-
 ?
4-Guanidinobutyrate + H2O
-
 Brevibacterium lipolyticum 4-Aminobutyrate + urea
-
 ?
4-Guanidinobutyrate + H2O
-
 Corynebacterium stationis 4-Aminobutyrate + urea
-
 ?
arginine + H2O weak activity towards L- and D-arginine Brevibacterium helvolum 2,5-diaminopentanoate + urea
-
 ?
L-canavanine + H2O
-
 Brevibacterium helvolum L-2-amino-4-(aminooxy)butanoic acid + urea
-
 ?
additional information
-
 Streptomyces griseus ?
-
 ?
additional information guanidinoacetate, L-guanidinosuccinate, 6-guanidinocaproate, 2-guanidinoethyl sulfate, 4-guanidinobutyramide, taurocyamine, creatine, methylguanidine, N-amidino-L-alanine and agmatine are inert as substrates Brevibacterium helvolum ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 65000, room temperature, SDS-PAGE, compactly associated subunits Brevibacterium helvolum
tetramer 4 * 45000, SDS-PAGE Brevibacterium helvolum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 residual activity after heating for 60 min 50% of the original activity Brevibacterium helvolum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Brevibacterium helvolum