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Literature summary for 3.5.3.7 extracted from

  • Arakawa, N.; Igarashi, M.; Kazuoka, T.; Oikawa, T.; Soda, K.
    D-arginase of Arthrobacter sp. KUJ 8602: characterization and its identity with Zn(2+)-guanidinobutyrase (2003), J. Biochem., 133, 33-42.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli Arthrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline inactivation due to removal of bound Zn2+, reversible by Zn2+ and Co2+, and partially by Mn2+ Arthrobacter sp.
5-Aminovalerate competitive inhibition Arthrobacter sp.
Mercaptoacetate strong mixed-type inhibitor Arthrobacter sp.
additional information inhibition by thiol carboxylate, metal binding sequence Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Arthrobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Arthrobacter sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can substitute for Zn2+ Arthrobacter sp.
Mn2+ can partially substitute for Zn2+ Arthrobacter sp.
Zn2+ 1 g-atom bound to 1 mol of subunit, dependent on, removal causes loss of activity Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
6 * 40000, SDS-PAGE Arthrobacter sp.
232000
-
gel filtration Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. Q8KZT5 strain KUJ8602
-
Arthrobacter sp. KUJ 8602 Q8KZT5 strain KUJ8602
-

Purification (Commentary)

Purification (Comment) Organism
113fold Arthrobacter sp.

Reaction

Reaction Comment Organism Reaction ID
4-guanidinobutanoate + H2O = 4-aminobutanoate + urea enzyme is identical with D-arginase, EC 3.5.3.10, substrate guanidino group binding sequence Arthrobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture-condition:L-arginine-grown cell
-
Arthrobacter sp.
-
additional information the cells can use D-arginine as sole carbon and nitrogen source Arthrobacter sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.71
-
purified enzyme Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-guanidinopropionate + H2O
-
Arthrobacter sp. 3-aminopropanoate + urea
-
?
4-Guanidinobutyrate + H2O preferred substrate Arthrobacter sp. 4-Aminobutyrate + urea
-
?
4-Guanidinobutyrate + H2O preferred substrate Arthrobacter sp. KUJ 8602 4-Aminobutyrate + urea
-
?
D-arginine + H2O
-
Arthrobacter sp. D-ornithine + urea
-
?
L-arginine + H2O
-
Arthrobacter sp. L-ornithine + urea
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 40000, SDS-PAGE Arthrobacter sp.

Synonyms

Synonyms Comment Organism
4-guanidinobutyrate amidinobutyrase
-
Arthrobacter sp.
GBase
-
Arthrobacter sp.
More enzyme is identical with D-arginase, EC 3.5.3.10 Arthrobacter sp.
Zn2+-guanidinobutyrase
-
Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
with substrate 4-guanidinobutyrate Arthrobacter sp.
9.5
-
with substrate D-arginine Arthrobacter sp.