Information on EC 3.5.1.60 - N-(long-chain-acyl)ethanolamine deacylase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.5.1.60
-
RECOMMENDED NAME
GeneOntology No.
N-(long-chain-acyl)ethanolamine deacylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-(long-chain-acyl)ethanolamine + H2O = a long-chain carboxylate + ethanolamine
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amides
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
N-(long-chain-acyl)ethanolamine amidohydrolase
Does not act on N-acylsphingosine or N,O-diacylethanolamine.
CAS REGISTRY NUMBER
COMMENTARY hide
99283-61-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
mouse
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
-
the catalytic triad, is formed by cysteine, aspartate and arginine
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
heptadecenoylethanolamide + H2O
heptadecenoic acid + ethanolamine
show the reaction diagram
-
-
-
-
?
N-(4-methylcoumarin)palmitamide + H2O
palmitic acid + 7-amino-4-methylcoumarin
show the reaction diagram
N-arachidonoylethanolamine + H2O
arachidic acid + ethanolamine
show the reaction diagram
N-dodecanoylethanolamine + H2O
dodecanoic acid + ethanolamine
show the reaction diagram
-
110% of activity compared to N-oleoylethanolamine
-
?
N-hexadecanoylethanolamine + H2O
hexadecanoic acid + ethanolamine
show the reaction diagram
-
71% of activity compared to N-oleoylethanolamine
-
?
N-octadecanoylethanolamine + H2O
octadecanoic acid + ethanolamine
show the reaction diagram
-
42% of activity compared to N-oleoylethanolamine
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
show the reaction diagram
-
best substrate in mixture of N-acylethanolamines
-
r
N-palmitoylethanolamine + H2O
palmitic acid + ethanolamine
show the reaction diagram
-
-
-
-
?
N-tetradecanoylethanolamine + H2O
tetradecanoic acid + ethanolamine
show the reaction diagram
-
95% of activity compared to N-oleoylethanolamine
-
?
oleamide + H2O
oleic acid + NH3
show the reaction diagram
-
-
-
?
oleoylethanolamide + H2O
oleic acid + ethanolamine
show the reaction diagram
palmitoylethanolamide + H2O
palmitic acid + ethanolamine
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-arachidonoylethanolamine + H2O
arachidic acid + ethanolamine
show the reaction diagram
oleoylethanolamide + H2O
oleic acid + ethanolamine
show the reaction diagram
palmitoylethanolamide + H2O
palmitic acid + ethanolamine
show the reaction diagram
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(7E)-N-[(3S)-2-oxoazetidin-3-yl]non-7-enamide
-
-
(S)-2-oxo-3-oxetanyl-carbamic acid benzyl ester
-
a serine-derived beta-lactone, weak inhibition of rat lung enzyme, structure-activity relationship studies confirm that the ability of the compound to inhibit the enzyme depends on the beta-lactone ring, rather than the carbamate fragment, because analogues lacking the beta-lactone moiety are devoid of inhibitory activity
(Z)-N-[(S)-2-oxoazetidin-3-yl]non-3-enamide
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-
([1,1'-biphenyl]-4-yl)methyl cyclopentanecarboxylate
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([1,1'-biphenyl]-4-yl)methyl [(2S,3R)-2-methyl-4-oxooxetan-3-yl]carbamate
1-(2-(4-benzyloxy)phenyl)ethyl-carbonyl pyrrolidine
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1-(2-biphenyl-4-yl)ethyl-carbonyl pyrrolidine
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1-(2-naphthalenyl)acetyl pyrrolidine
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1-(2-naphthalenyl)carbonyl pyrrolidine
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1-(2-phenylethyl)-carbonyl pyrrolidine
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1-(3-phenylpropanyl)-carbonyl pyrrolidine
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1-(4-benzyloxy)benzyl-carbonyl pyrrolidine
-
-
1-(4-phenylbenzyl)-carbonyl pyrrolidine
-
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1-(4-phenylbutanyl)-carbonyl pyrrolidine
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1-(5-phenylpentanyl)-carbonyl pyrrolidine
-
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1-(6-phenylhexanyl)-carbonyl pyrrolidine
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-
1-(7-phenylheptanyl)-carbonyl pyrrolidine
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1-(biphenyl-4-ylcarbonyl)pyrrolidine
-
-
1-benzyl-carbonyl pyrrolidine
-
-
1-heptyl-3-[(S)-2-oxoazetidin-3-yl]urea
-
-
1-hexadecanoylpyrrolidine
-
weak inhibition
-
1-isothiocyanatopentadecane
-
1-pentadecanyl-carbonyl piperidine
-
-
1-pentadecanyl-carbonyl pyrrole
-
-
1-pentadecanyl-carbonyl pyrrolidine
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1-[3-([1,1'-biphenyl]-4-yl)propyl]pyrrolidine
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2,2-dimethyl-N-[(3S)-2-oxoazetidin-3-yl]nonanamide
-
-
2-(dodecyloxy)ethan-1-amine
-
40% remaining activity
2-(hexadecyloxy)ethan-1-amine
-
91% remaining activity
2-(tetradecyloxy)ethan-1-amine
-
48% remaining activity
2-aminoethyl decanoate
-
89% remaining activity
2-aminoethyl dodecanoate
-
74% remaining activity
2-aminoethyl hexadecanoate
-
50% remaining activity
2-aminoethyl tetradecanoate
-
46% remaining activity
2-methyl-N-[(3S)-2-oxoazetidin-3-yl]nonanamide
-
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3-(benzyloxy)propyl [(2S,3R)-2-methyl-4-oxooxetan-3-yl]carbamate
3-(dodecyloxy)propan-1-amine
-
73% remaining activity
3-(hexadecyloxy)propan-1-amine
-
84% remaining activity
3-(tetradecyloxy)propan-1-amine
-
71% remaining activity
3-([1,1'-biphenyl]-4-yl)-1-(pyrrolidin-1-yl)propan-1-one
3-amino-N-nonanoyl-L-alanine
-
-
3-aminopropyl decanoate
-
55% remaining activity
3-aminopropyl dodecanoate
-
63% remaining activity
3-aminopropyl hexadecanoate
-
48% remaining activity
3-aminopropyl tetradecanoate
-
46% remaining activity
4-butyl-N-[(S)-2-oxoazetidin-3-yl]benzamide
-
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
0.5 mM: 50% inhibition of mitochondrial, 42% inhibition of microsomal enzyme
5-((biphenyl-4-yl)methyl)-N,N-dimethyl-2H-tetrazole-2-carboxamide
-
inhibits the enzyme in a covalent and irreversible manner
5-cyclohexylpentyl [(2S,3R)-2-methyl-4-oxooxetan-3-yl]carbamate
5-phenylpentyl N-[(2S,3R)-2-methyl-4-oxo-oxetan-3-yl]-carbamate
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-
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5-phenylpentyl [(2S,3R)-2-methyl-4-oxooxetan-3-yl]carbamate
7-cyclohexyl-N-[(3S)-2-oxoazetidin-3-yl]heptanamide
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benzyl [(2R,3S)-2-methyl-4-oxooxetan-3-yl]carbamate
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benzyl [(2S,3R)-2-methyl-4-oxooxetan-3-yl]carbamate
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biphenyl-4-ylmethyl pyrrolidinyl-1-carboxylate
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cyclobutanol
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41% inhibition at 0.05 mM
Cyclopentanol
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85% inhibition at 0.05 mM
cyclopentyl hexadecanoate
-
competitive inhibition
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cyclopentyl palmitate
-
-
dodecyl 2-aminoacetate
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34% remaining activity
dodecyl 3-aminopropanoate
-
51% remaining activity
heptyl N-[(S)-2-oxoazetidin-3-yl]carbamate
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hexadecyl 2-aminoacetate
-
71% remaining activity
hexadecylamine
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-
N-(2-oxocyclobutyl)nonanamide
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N-(azetidin-3-yl)nonanamide
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N-(biphenyl-4-ylmethyl)pyrrolidinyl-1-carboxamide
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N-benzyloxycarbonyl-L-serine beta-lactone
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inhibits the enzyme in a covalent and irreversible manner
N-cyclopentylpalmitamide
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-
N-ethylmaleimide
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0.25 mM: 50% inhibition of mitochondrial enzyme, 0.5 mM: 50% inhibition of microsomal enzyme
N-pentadecylbenzamide
-
a potent and selective inhibitor
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N-pentadecylcyclohexancarboxamide
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N-[(2R,3S)-2-methyl-4-oxooxetan-3-yl]-3-phenylpropanamide
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very low inhibition
N-[(2S,3R)-2-methyl-4-oxooxetan-3-yl]-3-phenylpropanamide
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-
N-[(3R)-2-oxo-3-oxetanyl]-3-phenylpropanamide
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weak inhibition
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N-[(3R)-2-oxoazetidin-3-yl]nonanamide
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N-[(3S)-2-oxo-3-oxetanyl]-3-phenylpropanamide
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N-[(3S)-2-oxoazetidin-3-yl]-4-phenylbutanamide
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-
N-[(3S)-2-oxoazetidin-3-yl]-5-phenylpentanamide
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-
N-[(3S)-2-oxoazetidin-3-yl]-6-phenylhexanamide
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-
N-[(3S)-2-oxoazetidin-3-yl]decanamide
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-
N-[(3S)-2-oxoazetidin-3-yl]heptanamide
-
-
N-[(3S)-2-oxoazetidin-3-yl]nonanamide
-
-
N-[(3S)-2-oxoazetidin-3-yl]octanamide
-
-
N-[(3S)-2-oxoazetidin-3-yl]undecanamide
-
-
N-[(3S)-2-oxooxetan-3-yl]heptanamide
-
-
N-[(3S)-2-oxooxetan-3-yl]naphthalene-2-carboxamide
-
-
N-[(3S)-2-oxooxetan-3-yl][1,1'-biphenyl]-4-carboxamide
-
-
N-[(S)-2-oxoazetidin-3-yl]-6-phenylhexanamide
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N-[([1,1'-biphenyl]-4-yl)methyl]cyclopentanecarboxamide
-
-
oleic acid
-
10 nM: 50% inhibition, 100 nM: 95% inhibition
p-chloromercuribenzoate
-
0.05 mM: total inhibition of mitochondrial or microsomal enzyme
palmitic acid retro-amides N-pentadecylbenzamide
-
a potent and selective inhibitor
-
pentadecyl 2-aminoacetate
-
42% remaining activity
pentadecylamine
sodium cholate
-
7.5 mg/ml: 90% inhibition
Sodium dodecylcholate
-
2.5 mg/ml: 98% inhibition
-
sodium taurodeoxycholate
-
15 mg/ml: 20% inhibition
tetradecyl 2-aminoacetate
-
16% remaining activity
tetradecyl 3-aminopropanoate
-
30% remaining activity
tetradecylamine
-
-
tetrahydrofuran-3-yl palmitate
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-
tridecyl 2-aminoacetate
-
11% remaining activity
tridecyl 3-aminopropanoate
-
29% remaining activity
tridecylglycine
-
competitive inhibition
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Triton X-100
-
5 mg/ml: 42% inhibition
undecylamine
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
the enzyme is proteolytically activated by an autocatalytic step under acidic conditions where the polypeptide is cleaved into two chains
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0062
N-(4-methylcoumarin)palmitamide
-
0.025 - 0.05
N-oleoylethanolamine
-
mitochondrial enzyme
0.021
N-palmitoylethanolamine
-
recombinant enzyme, pH not specified in the publication, 37°C
0.009
oleamide
-
-
0.021
palmitoylethanolamide
-
pH 4.5, 37°C
additional information
additional information
-
Michaelis-Menten kinetics
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5
5,5'-dithiobis(2-nitrobenzoic acid)
-
mitochondrial
0.000013
5-((biphenyl-4-yl)methyl)-N,N-dimethyl-2H-tetrazole-2-carboxamide
-
recombinant enzyme, pH not specified in the publication, 37°C
0.0013
N-benzyloxycarbonyl-L-serine beta-lactone
-
recombinant enzyme, pH not specified in the publication, 37°C
25
N-ethylmaleimide
-
mitochondrial enzyme
0.000001
oleic acid
-
-
0.0057
pentadecylamine
-
pH 5.0, 37°C
0.0118
tridecyl 2-aminoacetate
-
pH 5.0, 37°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00309
(7E)-N-[(3S)-2-oxoazetidin-3-yl]non-7-enamide
Homo sapiens
-
pH 5.0, 37°C
0.00296
(S)-2-oxo-3-oxetanyl-carbamic acid benzyl ester
Rattus norvegicus
-
pH and temperature not specified in the publication
0.0039
(Z)-N-[(S)-2-oxoazetidin-3-yl]non-3-enamide
Homo sapiens
-
pH 5.0, 37°C
0.000007
([1,1'-biphenyl]-4-yl)methyl [(2S,3R)-2-methyl-4-oxooxetan-3-yl]carbamate
0.00576
1-heptyl-3-[(S)-2-oxoazetidin-3-yl]urea
Homo sapiens
-
pH 5.0, 37°C
0.025
1-hexadecanoylpyrrolidine
Rattus norvegicus
-
pH and temperature not specified in the publication
-
0.00035 - 0.0006
1-isothiocyanatopentadecane
-
0.00076
2,2-dimethyl-N-[(3S)-2-oxoazetidin-3-yl]nonanamide
Homo sapiens
-
pH 5.0, 37°C
0.00022
2-methyl-N-[(3S)-2-oxoazetidin-3-yl]nonanamide
Homo sapiens
-
pH 5.0, 37°C
0.00212
3-([1,1'-biphenyl]-4-yl)-1-(pyrrolidin-1-yl)propan-1-one
Rattus norvegicus
-
pH and temperature not specified in the publication
0.0138
4-butyl-N-[(S)-2-oxoazetidin-3-yl]benzamide
Homo sapiens
-
pH 5.0, 37°C
0.0000072 - 0.0000077
5-((biphenyl-4-yl)methyl)-N,N-dimethyl-2H-tetrazole-2-carboxamide
0.05
5-phenylpentyl N-[(2S,3R)-2-methyl-4-oxo-oxetan-3-yl]-carbamate
Homo sapiens
-
pH 4.5, 37°C
-
0.000007 - 0.05
5-phenylpentyl [(2S,3R)-2-methyl-4-oxooxetan-3-yl]carbamate
0.00028
7-cyclohexyl-N-[(3S)-2-oxoazetidin-3-yl]heptanamide
Homo sapiens
-
pH 5.0, 37°C
0.01
benzyl [(2R,3S)-2-methyl-4-oxooxetan-3-yl]carbamate
Rattus norvegicus
-
above, pH and temperature not specified in the publication
0.001
benzyl [(2S,3R)-2-methyl-4-oxooxetan-3-yl]carbamate
Rattus norvegicus
-
pH and temperature not specified in the publication
0.01
cyclopentyl hexadecanoate
Homo sapiens
-
pH and temperature not specified in the publication
-
0.00024
heptyl N-[(S)-2-oxoazetidin-3-yl]carbamate
Homo sapiens
-
pH 5.0, 37°C
0.0017 - 0.0019
N-benzyloxycarbonyl-L-serine beta-lactone
0.0083
N-pentadecylbenzamide
Rattus norvegicus
-
pH and temperature not specified in the publication
-
0.0045
N-pentadecylcyclohexancarboxamide
Rattus norvegicus
-
pH and temperature not specified in the publication
-
0.1
N-[(2R,3S)-2-methyl-4-oxooxetan-3-yl]-3-phenylpropanamide
Rattus norvegicus
-
above, pH and temperature not specified in the publication
0.0032
N-[(2S,3R)-2-methyl-4-oxooxetan-3-yl]-3-phenylpropanamide
Rattus norvegicus
-
pH and temperature not specified in the publication
0.006
N-[(3R)-2-oxo-3-oxetanyl]-3-phenylpropanamide
Rattus norvegicus
-
pH and temperature not specified in the publication
-
0.0745
N-[(3R)-2-oxoazetidin-3-yl]nonanamide
Homo sapiens
-
pH 5.0, 37°C
0.00042
N-[(3S)-2-oxo-3-oxetanyl]-3-phenylpropanamide
Rattus norvegicus
-
pH and temperature not specified in the publication
-
0.0734
N-[(3S)-2-oxoazetidin-3-yl]-4-phenylbutanamide
Homo sapiens
-
pH 5.0, 37°C
0.0272
N-[(3S)-2-oxoazetidin-3-yl]-5-phenylpentanamide
Homo sapiens
-
pH 5.0, 37°C
0.0006
N-[(3S)-2-oxoazetidin-3-yl]-6-phenylhexanamide
Homo sapiens
-
pH 5.0, 37°C
0.00024
N-[(3S)-2-oxoazetidin-3-yl]decanamide
Homo sapiens
-
pH 5.0, 37°C
0.027
N-[(3S)-2-oxoazetidin-3-yl]heptanamide
Homo sapiens
-
pH 5.0, 37°C
0.00034
N-[(3S)-2-oxoazetidin-3-yl]nonanamide
Homo sapiens
-
pH 5.0, 37°C
0.0019
N-[(3S)-2-oxoazetidin-3-yl]octanamide
Homo sapiens
-
pH 5.0, 37°C
0.0001
N-[(3S)-2-oxoazetidin-3-yl]undecanamide
Homo sapiens
-
pH 5.0, 37°C
0.00046
N-[(3S)-2-oxooxetan-3-yl]heptanamide
Rattus norvegicus
-
pH and temperature not specified in the publication
0.00016
N-[(3S)-2-oxooxetan-3-yl]naphthalene-2-carboxamide
Rattus norvegicus
-
pH and temperature not specified in the publication
0.000115
N-[(3S)-2-oxooxetan-3-yl][1,1'-biphenyl]-4-carboxamide
Rattus norvegicus
-
pH and temperature not specified in the publication
0.0121
N-[(S)-2-oxoazetidin-3-yl]-6-phenylhexanamide
Homo sapiens
-
pH 5.0, 37°C
0.0057
pentadecylamine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
-
assay at
10.5
-
reverse reaction
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10972
-
1 * 10972, deglycosylated alpha-subunit, + 1 * 29659, deglycosylated beta-subunit, mass spectrometry, 1 * 14600 + 1 * 33000, alpha/beta heterodimer, SDS-PAGE
14600
-
1 * 10972, deglycosylated alpha-subunit, + 1 * 29659, deglycosylated beta-subunit, mass spectrometry, 1 * 14600 + 1 * 33000, alpha/beta heterodimer, SDS-PAGE
29659
-
1 * 10972, deglycosylated alpha-subunit, + 1 * 29659, deglycosylated beta-subunit, mass spectrometry, 1 * 14600 + 1 * 33000, alpha/beta heterodimer, SDS-PAGE
33000
-
1 * 10972, deglycosylated alpha-subunit, + 1 * 29659, deglycosylated beta-subunit, mass spectrometry, 1 * 14600 + 1 * 33000, alpha/beta heterodimer, SDS-PAGE
40593
-
1 * 40593, deglycosylated zymogen, mass spectrometry
45000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
-
1 * 10972, deglycosylated alpha-subunit, + 1 * 29659, deglycosylated beta-subunit, mass spectrometry, 1 * 14600 + 1 * 33000, alpha/beta heterodimer, SDS-PAGE
monomer
-
1 * 40593, deglycosylated zymogen, mass spectrometry
additional information
-
tryptic digestion and MALDI-TOF mass spectrometry fingerprinting gives evidence for the lack of a disulfide bond between subunits
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
4 glycosylation sites Asn37, Asn107, Asn309, and Asn333, N-linked oligosaccharides of approximately 1500 Da, all cleavable by peptide-N-glycosidase F
proteolytic modification
-
the glycoprotein undergoes removal of an N-terminal signal peptide after biosynthesis. Autocatalytic acid cleavage of the zymogen into alpha- and beta-subunits (14.6 and 33.3 kDa) activates the enzyme. Cys126 is essential for the proteolytic cleavage of the pro-enzyme
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
bovine serum albumin stabilizes
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-40°C, solubilized enzyme, stable for several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant C-terminally His6-tagged enzyme from HEK-293 cells by nickel affinity chromatography
-
recombinant His6-tagged zymogen from HEK-293 cells by ammonium sulfate fractionation, dialysis, metal affinity chromatography, and again dialysis followed by ultrafiltration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in COS-7 cells
recombinant expression in HEK-293 cells
-
recombinant expression of the C-terminally His6-tagged enzyme in HEK-293 cells
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recombinant expression of the enzyme in HEK-293 cells
-
recombinant overexpression in HEK-293 cell membranes
recombinant stable expression of the His6-tagged zymogen in HEK-293 cells, ammonium chloride treatment stimulates secretion of the lysosomal proteins from the HEK293 cells
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
selective alkylation of the enzyme's cysteine residues results in almost complete loss of activity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
medicine
-
modulation of the tissue levels of palmitoylethanolamide by inhibition of enzymes responsible for the breakdown of this lipid mediator, including the N-acylethanolamine acid amidase, may represent therefore a therapeutic strategy for the treatment of pain and inflammation
pharmacology
-
potential of blocking N-acylethanolamines like palmitoylethanolamide or N-arachidonlyethanolamine (anandamide) from enzymatic degradation via enzyme inhibition as a strategy for pain treatment