General Stability | Organism |
---|---|
bovine serum albumin stabilizes | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | 0.5 mM: 50% inhibition of mitochondrial, 42% inhibition of microsomal enzyme | Rattus norvegicus | |
N-ethylmaleimide | 0.25 mM: 50% inhibition of mitochondrial enzyme, 0.5 mM: 50% inhibition of microsomal enzyme | Rattus norvegicus | |
oleic acid | 10 nM: 50% inhibition, 100 nM: 95% inhibition | Rattus norvegicus | |
p-chloromercuribenzoate | 0.05 mM: total inhibition of mitochondrial or microsomal enzyme | Rattus norvegicus | |
sodium cholate | 7.5 mg/ml: 90% inhibition | Rattus norvegicus | |
sodium dodecylsulfate | 2.5 mg/ml: 98% inhibition | Rattus norvegicus | |
sodium taurodeoxycholate | 15 mg/ml: 20% inhibition | Rattus norvegicus | |
Triton X-100 | 5 mg/ml: 42% inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | 0.05 | N-oleoylethanolamine | mitochondrial enzyme | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-40°C, solubilized enzyme, stable for several weeks | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | N-acylsphingosine or N,O-diacylethanolamine are no substrates | Rattus norvegicus | ? | - |
? | |
N-dodecanoylethanolamine + H2O | 110% of activity compared to N-oleoylethanolamine | Rattus norvegicus | dodecanoic acid + ethanolamine | - |
? | |
N-hexadecanoylethanolamine + H2O | 71% of activity compared to N-oleoylethanolamine | Rattus norvegicus | hexadecanoic acid + ethanolamine | - |
? | |
N-octadecanoylethanolamine + H2O | 42% of activity compared to N-oleoylethanolamine | Rattus norvegicus | octadecanoic acid + ethanolamine | - |
? | |
N-oleoylethanolamine + H2O | best substrate in mixture of N-acylethanolamines | Rattus norvegicus | oleic acid + ethanolamine | - |
r | |
N-tetradecanoylethanolamine + H2O | 95% of activity compared to N-oleoylethanolamine | Rattus norvegicus | tetradecanoic acid + ethanolamine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Rattus norvegicus |
10.5 | - |
reverse reaction | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000001 | - |
oleic acid | - |
Rattus norvegicus | |
0.5 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | mitochondrial | Rattus norvegicus | |
25 | - |
N-ethylmaleimide | mitochondrial enzyme | Rattus norvegicus |