Literature summary for 3.5.1.60 extracted from

West, J.M.; Zvonok, N.; Whitten, K.M.; Vadivel, S.K.; Bowman, A.L.; Makriyannis, A.
Biochemical and mass spectrometric characterization of human N-acylethanolamine-hydrolyzing acid amidase inhibition (2012), PLoS ONE, 7, e43877.

Search for more literature for 3.5.1.60

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of the C-terminally His6-tagged enzyme in HEK-293 cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
1-isothiocyanatopentadecane	a potent, selective and reversible inhibitor	Homo sapiens
5-((biphenyl-4-yl)methyl)-N,N-dimethyl-2H-tetrazole-2-carboxamide	inhibits the enzyme in a covalent and irreversible manner	Homo sapiens
additional information	mechanism of inactivation of human enzyme N-acylethanolamine-hydrolyzing acid amidase with selected inhibitors identified in a fluorescent based assay developed for characterization of both reversible and irreversible inhibitors, kinetic analysis using MALDI-TOF mass spectroscopy, molecular modeling, overview	Homo sapiens
N-benzyloxycarbonyl-L-serine beta-lactone	inhibits the enzyme in a covalent and irreversible manner	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0062	-	N-(4-methylcoumarin)palmitamide	recombinant enzyme, pH not specified in the publication, 37°C	Homo sapiens
0.021	-	N-palmitoylethanolamine	recombinant enzyme, pH not specified in the publication, 37°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q02083	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant C-terminally His6-tagged enzyme from HEK-293 cells by nickel affinity chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-(4-methylcoumarin)palmitamide + H2O	-	Homo sapiens	palmitic acid + 7-amino-4-methylcoumarin	-	?
N-palmitoylethanolamine + H2O	-	Homo sapiens	palmitic acid + ethanolamine	-	?

Synonyms

Synonyms	Comment	Organism
N-acylethanolamine-hydrolyzing acid amidase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000013	-	5-((biphenyl-4-yl)methyl)-N,N-dimethyl-2H-tetrazole-2-carboxamide	recombinant enzyme, pH not specified in the publication, 37°C	Homo sapiens
0.0013	-	N-benzyloxycarbonyl-L-serine beta-lactone	recombinant enzyme, pH not specified in the publication, 37°C	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0000072	-	recombinant enzyme with substrate N-palmitoylethanolamine, pH not specified in the publication, 37°C	Homo sapiens	5-((biphenyl-4-yl)methyl)-N,N-dimethyl-2H-tetrazole-2-carboxamide
0.0000077	-	recombinant enzyme with substrate N-(4-methyl coumarin)palmitamide, pH not specified in the publication, 37°C	Homo sapiens	5-((biphenyl-4-yl)methyl)-N,N-dimethyl-2H-tetrazole-2-carboxamide
0.00035	-	recombinant enzyme with substrate N-(4-methyl coumarin)palmitamide, pH not specified in the publication, 37°C	Homo sapiens	1-isothiocyanatopentadecane
0.0006	-	recombinant enzyme with substrate N-palmitoylethanolamine, pH not specified in the publication, 37°C	Homo sapiens	1-isothiocyanatopentadecane
0.0017	-	recombinant enzyme with substrate N-(4-methyl coumarin)palmitamide, pH not specified in the publication, 37°C	Homo sapiens	N-benzyloxycarbonyl-L-serine beta-lactone
0.0019	-	recombinant enzyme with substrate N-palmitoylethanolamine, pH not specified in the publication, 37°C	Homo sapiens	N-benzyloxycarbonyl-L-serine beta-lactone

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Release 2023.1 (January 2023)