Information on EC 3.5.1.42 - nicotinamide-nucleotide amidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.5.1.42
-
RECOMMENDED NAME
GeneOntology No.
nicotinamide-nucleotide amidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
beta-nicotinamide D-ribonucleotide + H2O = beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
Also acts more slowly on beta-nicotinamide D-ribonucleoside
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of carboxylic acid amide
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
NAD salvage pathway I
-
Nicotinate and nicotinamide metabolism
-
SYSTEMATIC NAME
IUBMB Comments
nicotinamide-D-ribonucleotide amidohydrolase
Also acts more slowly on beta-nicotinamide D-ribonucleoside.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
nicotinamide mononucleotide amidohydrolase
-
-
-
-
nicotinamide mononucleotide deamidase
-
-
-
-
nicotinamide mononucleotide deamidase
-
-
nicotinamide mononucleotide deaminase
-
-
-
-
nicotinamide nucleoside amidase
-
-
-
-
NMN deaminase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37355-58-1
-
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-
the only possible way of nicotinamide salvage in Shewanella oneidensis
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
-
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
ir
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
integral component of the four-membered pyridine nucleotide cycle
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
involvement in NAD metabolism
-
ir
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
involvement in NAD metabolism
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
contribution to the recycling pathway of NAD
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
contribution to the recycling pathway of NAD
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
contribution to the recycling pathway of NAD, highly specific, involvement in B12 synthesis and bacterial DNA ligase reaction
-
?
nicotinamide + H2O
nicotinate + NH3
show the reaction diagram
-
-
-
-
?
nicotinamide mononucleotide + H2O
nicotinate mononucleotide + NH3
show the reaction diagram
-
-
-
-
ir
nicotinamide mononucleotide + H2O
nicotinate mononucleotide + NH3
show the reaction diagram
-
-
-
-
?
nicotinamide mononucleotide + H2O
nicotinate mononucleotide + NH3
show the reaction diagram
-
-
-
-
ir
nicotinamide ribonucleoside + H2O
nicotinic acid ribonucleoside + NH3
show the reaction diagram
-
14% activity compared to nicotinamide D-ribonucleotide
-
ir
nicotinamide riboside + H2O
nicotinate riboside + NH3
show the reaction diagram
-
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
-
-
-
?
additional information
?
-
-
the enzyme has no detectable deamidase activity toward NAD, NADP+, nicotinamide, and nicotinamide riboside
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
-
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
integral component of the four-membered pyridine nucleotide cycle
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
involvement in NAD metabolism
-
ir
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
involvement in NAD metabolism
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
contribution to the recycling pathway of NAD
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
contribution to the recycling pathway of NAD
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
contribution to the recycling pathway of NAD, highly specific, involvement in B12 synthesis and bacterial DNA ligase reaction
-
?
nicotinamide mononucleotide + H2O
nicotinate mononucleotide + NH3
show the reaction diagram
-
-
-
-
ir
nicotinamide ribonucleoside + H2O
nicotinic acid ribonucleoside + NH3
show the reaction diagram
-
14% activity compared to nicotinamide D-ribonucleotide
-
ir
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
-
-
-
?
additional information
?
-
-
the enzyme has no detectable deamidase activity toward NAD, NADP+, nicotinamide, and nicotinamide riboside
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
-
significant stimulation
additional information
-
the enzyme does not require a divalent cation for catalytic activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
alpha-nicotinic acid adenine dinucleotide
-
non-competitive inhibitor, Ki: 8 mM
alpha-nicotinic acid mononucleotide
-
non-competitive inhibitor, Ki: 21 mM
beta-nicotinic acid adenine dinucleotide
-
non-competitive inhibitor, Ki: 7 mM
Cu2+
-
strong inhibitor
deamido-NAD+
-
product inhibition
Hg2+
-
52% inhibition at 1 mM
Isonicotinic acid
-
non-competitive inhibitor, Ki: 8.3 mM
nicotinic acid
-
non-competitive inhibitor, Ki: 4.4 mM
p-chloromercuribenzoate
-
-
p-hydroxymercuribenzoate
-
26% inhibition at 1 mM
Picolinic acid
-
non-competitive inhibitor, Ki: 9.8 mM
Quinolinic acid
-
non-competitive inhibitor, Ki: 3.9 mM
additional information
-
not affected by EDTA up to 10 mM, the enzyme retains its activity in the presence of 0.2 mM iodoacetamide
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
ATP
-
weak activator
diphosphate
-
weak activator
phosphate
-
weak activator
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3.6
-
beta-nicotinamide D-ribonucleoside
-
-
0.014
-
beta-nicotinamide D-ribonucleotide
-
-
0.07
-
beta-nicotinamide D-ribonucleotide
-
-
0.006
-
nicotinamide mononucleotide
-
in 100 mM potassium phosphate, pH 8.0, 10 mM sodium fluoride, 10 mM EDTA, at 37C
1.05
-
beta-nicotinamide D-ribonucleotide
-
-
additional information
-
additional information
-
beta-nicotinamide D-ribonucleotide, sigmoidal shape of substrate concentration shows a form of allosteric control
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3.3
-
nicotinamide mononucleotide
-
in 100 mM potassium phosphate, pH 8.0, 10 mM sodium fluoride, 10 mM EDTA, at 37C
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
8
-
alpha-nicotinic acid adenine dinucleotide
-
-
21
-
alpha-nicotinic acid mononucleotide
-
-
7
-
beta-nicotinic acid adenine dinucleotide
-
-
8.3
-
Isonicotinic acid
-
-
4.4
-
nicotinic acid
-
-
9.8
-
Picolinic acid
-
-
3.9
-
Quinolinic acid
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.003
-
-
crude extract, at pH 8.0 and 37C
0.07
-
-
cell-free extract
1.8
-
-
after 600fold purification, at pH 8.0 and 37C
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
10
-
the enzyme shows a broad optimum pH, ranging from 5.5 to 9.0, 72 and 60% residual activity is observed at pH 10.0 and 5.0, respectively
5
5.5
-
maximal activity within
5
-
-
no activity below
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25700
26300
-
gel filtration
33000
-
-
gel filtration
43000
-
-
gel filtration
80000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
homodimer
-
2 * about 20000, SDS-PAGE
homodimer
-
2 * 47000, SDS-PAGE
oligomer
-
heat-stable and heat-sensitive subunits
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
6
-
stable within for 30 min
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
64
-
-
preincubation without substrate: complete denaturation within 32 min
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
unstable during purification procedure
-
DTT required during purification
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4C, Tris-HCl, pH 7.5, 1 week
-
-15C, 0.5 mg protein/ml, 12% glycerol, 1 month, more stable at 4C in the presence of 24% glycerol
-
-70C, 20 mM Tris-HCl, pH 7.5, 15% glycerol, fully active within 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
to homogeneity, chromatography techniques
-
near homogeneity, chromatography techniques
-
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, Reactive Red 120-agarose column chromatography, Resource Q column chromatography, and Superose 12 gel filtration
-