Information on EC 3.5.1.42 - nicotinamide-nucleotide amidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.5.1.42
-
RECOMMENDED NAME
GeneOntology No.
nicotinamide-nucleotide amidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
beta-nicotinamide D-ribonucleotide + H2O = beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
Also acts more slowly on beta-nicotinamide D-ribonucleoside
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of carboxylic acid amide
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
NAD metabolism
-
-
NAD salvage pathway I
-
-
Nicotinate and nicotinamide metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
nicotinamide-D-ribonucleotide amidohydrolase
Also acts more slowly on beta-nicotinamide D-ribonucleoside.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
nicotinamide mononucleotide amidohydrolase
-
-
-
-
nicotinamide mononucleotide deamidase
-
-
-
-
nicotinamide mononucleotide deamidase
-
-
nicotinamide mononucleotide deaminase
-
-
-
-
nicotinamide nucleoside amidase
-
-
-
-
NMN deaminase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37355-58-1
-
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
-
the only possible way of nicotinamide salvage in Shewanella oneidensis
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
-
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
ir
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
integral component of the four-membered pyridine nucleotide cycle
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
involvement in NAD metabolism
-
ir
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
involvement in NAD metabolism
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
contribution to the recycling pathway of NAD
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
contribution to the recycling pathway of NAD
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
contribution to the recycling pathway of NAD, highly specific, involvement in B12 synthesis and bacterial DNA ligase reaction
-
?
nicotinamide + H2O
nicotinate + NH3
show the reaction diagram
-
-
-
-
?
nicotinamide mononucleotide + H2O
nicotinate mononucleotide + NH3
show the reaction diagram
-
-
-
-
ir
nicotinamide mononucleotide + H2O
nicotinate mononucleotide + NH3
show the reaction diagram
-
-
-
-
?
nicotinamide mononucleotide + H2O
nicotinate mononucleotide + NH3
show the reaction diagram
-
-
-
-
ir
nicotinamide ribonucleoside + H2O
nicotinic acid ribonucleoside + NH3
show the reaction diagram
-
14% activity compared to nicotinamide D-ribonucleotide
-
ir
nicotinamide riboside + H2O
nicotinate riboside + NH3
show the reaction diagram
-
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
-
-
-
?
additional information
?
-
-
the enzyme has no detectable deamidase activity toward NAD, NADP+, nicotinamide, and nicotinamide riboside
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
-
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
-
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
integral component of the four-membered pyridine nucleotide cycle
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
involvement in NAD metabolism
-
ir
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
involvement in NAD metabolism
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
contribution to the recycling pathway of NAD
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
contribution to the recycling pathway of NAD
-
?
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
-
contribution to the recycling pathway of NAD, highly specific, involvement in B12 synthesis and bacterial DNA ligase reaction
-
?
nicotinamide mononucleotide + H2O
nicotinate mononucleotide + NH3
show the reaction diagram
-
-
-
-
ir
nicotinamide ribonucleoside + H2O
nicotinic acid ribonucleoside + NH3
show the reaction diagram
-
14% activity compared to nicotinamide D-ribonucleotide
-
ir
beta-nicotinamide D-ribonucleotide + H2O
beta-nicotinate D-ribonucleotide + NH3
show the reaction diagram
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
-
-
-
?
additional information
?
-
-
the enzyme has no detectable deamidase activity toward NAD, NADP+, nicotinamide, and nicotinamide riboside
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
-
significant stimulation
additional information
-
the enzyme does not require a divalent cation for catalytic activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
alpha-nicotinic acid adenine dinucleotide
-
non-competitive inhibitor, Ki: 8 mM
alpha-nicotinic acid mononucleotide
-
non-competitive inhibitor, Ki: 21 mM
beta-nicotinic acid adenine dinucleotide
-
non-competitive inhibitor, Ki: 7 mM
Cu2+
-
strong inhibitor
deamido-NAD+
-
product inhibition
Hg2+
-
52% inhibition at 1 mM
Isonicotinic acid
-
non-competitive inhibitor, Ki: 8.3 mM
nicotinic acid
-
non-competitive inhibitor, Ki: 4.4 mM
p-chloromercuribenzoate
-
-
p-hydroxymercuribenzoate
-
26% inhibition at 1 mM
Picolinic acid
-
non-competitive inhibitor, Ki: 9.8 mM
quinolinic acid
-
non-competitive inhibitor, Ki: 3.9 mM
additional information
-
not affected by EDTA up to 10 mM, the enzyme retains its activity in the presence of 0.2 mM iodoacetamide
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ATP
-
weak activator
diphosphate
-
weak activator
phosphate
-
weak activator
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3.6
beta-nicotinamide D-ribonucleoside
-
-
0.014
beta-nicotinamide D-ribonucleotide
-
-
0.07
beta-nicotinamide D-ribonucleotide
-
-
0.006
nicotinamide mononucleotide
-
in 100 mM potassium phosphate, pH 8.0, 10 mM sodium fluoride, 10 mM EDTA, at 37C
1.05
beta-nicotinamide D-ribonucleotide
-
-
additional information
additional information
-
beta-nicotinamide D-ribonucleotide, sigmoidal shape of substrate concentration shows a form of allosteric control
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3.3
nicotinamide mononucleotide
-
in 100 mM potassium phosphate, pH 8.0, 10 mM sodium fluoride, 10 mM EDTA, at 37C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
8
alpha-nicotinic acid adenine dinucleotide
-
-
21
alpha-nicotinic acid mononucleotide
-
-
7
beta-nicotinic acid adenine dinucleotide
-
-
8.3
Isonicotinic acid
-
-
4.4
nicotinic acid
-
-
9.8
Picolinic acid
-
-
3.9
quinolinic acid
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.003
-
crude extract, at pH 8.0 and 37C
0.07
-
cell-free extract
1.8
-
after 600fold purification, at pH 8.0 and 37C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 10
-
the enzyme shows a broad optimum pH, ranging from 5.5 to 9.0, 72 and 60% residual activity is observed at pH 10.0 and 5.0, respectively
5 - 5.5
-
maximal activity within
5
-
no activity below
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25700 - 26300
-
gel filtration
209140
33000
-
gel filtration
209134
43000
-
gel filtration
719984
80000
-
gel filtration
719984
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
homodimer
-
2 * 47000, SDS-PAGE
homodimer
-
2 * about 20000, SDS-PAGE
oligomer
-
heat-stable and heat-sensitive subunits
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3.8
-
21% retaining activity
209139
5 - 6
-
stable within for 30 min
209134
7.4
-
48% retaining activity
209139
10
-
25% retaining activity
209139
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
64
-
preincubation without substrate: complete denaturation within 32 min
209140
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable during purification procedure
-
DTT required during purification
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, Tris-HCl, pH 7.5, 1 week
-
-15C, 0.5 mg protein/ml, 12% glycerol, 1 month, more stable at 4C in the presence of 24% glycerol
-
-70C, 20 mM Tris-HCl, pH 7.5, 15% glycerol, fully active within 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, chromatography techniques
-
near homogeneity, chromatography techniques
-
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, Reactive Red 120-agarose column chromatography, Resource Q column chromatography, and Superose 12 gel filtration
-