Cloned (Comment) | Organism |
---|---|
gene pncC, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) | Oceanobacillus iheyensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Oceanobacillus iheyensis | |
0.18 | - |
beta-nicotinamide D-ribonucleotide | recombinant enzyme, pH 7.4, 37°C | Oceanobacillus iheyensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47320 | - |
2 * 47320, recombinant His6-tagged enzyme, mass spectrometry | Oceanobacillus iheyensis |
96100 | - |
recombinant His6-tagged enzyme, gel filtration | Oceanobacillus iheyensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-nicotinamide D-ribonucleotide + H2O | Oceanobacillus iheyensis | absolute specific for | beta-nicotinate D-ribonucleotide + NH3 | - |
? | |
beta-nicotinamide D-ribonucleotide + H2O | Oceanobacillus iheyensis HTE831 | absolute specific for | beta-nicotinate D-ribonucleotide + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oceanobacillus iheyensis | Q8EQR8 | gene pncC | - |
Oceanobacillus iheyensis HTE831 | Q8EQR8 | gene pncC | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and gel filtration | Oceanobacillus iheyensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-nicotinamide D-ribonucleotide + H2O | absolute specific for | Oceanobacillus iheyensis | beta-nicotinate D-ribonucleotide + NH3 | - |
? | |
beta-nicotinamide D-ribonucleotide + H2O | absolute specific for | Oceanobacillus iheyensis HTE831 | beta-nicotinate D-ribonucleotide + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 47320, recombinant His6-tagged enzyme, mass spectrometry | Oceanobacillus iheyensis |
Synonyms | Comment | Organism |
---|---|---|
nicotinamide mononucleotide deamidase | - |
Oceanobacillus iheyensis |
NMN deamidase | - |
Oceanobacillus iheyensis |
OiPncC | - |
Oceanobacillus iheyensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Oceanobacillus iheyensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant enzyme, pH 7.3, stable up to | Oceanobacillus iheyensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
beta-nicotinamide D-ribonucleotide | recombinant enzyme, pH 7.4, 37°C | Oceanobacillus iheyensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Oceanobacillus iheyensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | the enzyme is active over a broad pH range, with an optimum at pH 7.4, whilst maintaining 90% activity at pH 10.0 | Oceanobacillus iheyensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10 | - |
purified recombinant enzyme, 37°C, maintains 61% activity after 21 days | Oceanobacillus iheyensis |
General Information | Comment | Organism |
---|---|---|
metabolism | nicotinamide mononucleotide (NMN) deamidase is one of the key enzymes of the bacterial pyridine nucleotide cycle, PNC | Oceanobacillus iheyensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
beta-nicotinamide D-ribonucleotide | recombinant enzyme, pH 7.4, 37°C | Oceanobacillus iheyensis |