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Literature summary for 3.5.1.42 extracted from

  • Galeazzi, L.; Bocci, P.; Amici, A.; Brunetti, L.; Ruggieri, S.; Romine, M.; Reed, S.; Osterman, A.L.; Rodionov, D.A.; Sorci, L.; Raffaelli, N.
    Identification of nicotinamide mononucleotide deamidase of the bacterial pyridine nucleotide cycle reveals a novel broadly conserved amidohydrolase family (2011), J. Biol. Chem., 286, 40365-40375.
    View publication on PubMedView publication on EuropePMC
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Inhibitors

Inhibitors Comment Organism Structure
additional information not affected by EDTA up to 10 mM, the enzyme retains its activity in the presence of 0.2 mM iodoacetamide Shewanella oneidensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 nicotinamide mononucleotide in 100 mM potassium phosphate, pH 8.0, 10 mM sodium fluoride, 10 mM EDTA, at 37°C Escherichia coli
0.006
-
 nicotinamide mononucleotide in 100 mM potassium phosphate, pH 8.0, 10 mM sodium fluoride, 10 mM EDTA, at 37°C Shewanella oneidensis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require a divalent cation for catalytic activity Shewanella oneidensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 gel filtration Escherichia coli
47000
-
 2 * 47000, SDS-PAGE Shewanella oneidensis
80000
-
 gel filtration Shewanella oneidensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Shewanella oneidensis the enzyme has no detectable deamidase activity toward NAD, NADP+, nicotinamide, and nicotinamide riboside ?
-
 ?
nicotinamide mononucleotide + H2O Escherichia coli
-
 nicotinate mononucleotide + NH3
-
 ir
nicotinamide mononucleotide + H2O Shewanella oneidensis
-
 nicotinate mononucleotide + NH3
-
 ir

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Shewanella oneidensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, Reactive Red 120-agarose column chromatography, Resource Q column chromatography, and Superose 12 gel filtration Shewanella oneidensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.003
-
 crude extract, at pH 8.0 and 37°C Shewanella oneidensis
1.8
-
 after 600fold purification, at pH 8.0 and 37°C Shewanella oneidensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme has no detectable deamidase activity toward NAD, NADP+, nicotinamide, and nicotinamide riboside Shewanella oneidensis ?
-
 ?
nicotinamide mononucleotide + H2O
-
 Escherichia coli nicotinate mononucleotide + NH3
-
 ir
nicotinamide mononucleotide + H2O
-
 Shewanella oneidensis nicotinate mononucleotide + NH3
-
 ir

Subunits

Subunits Comment Organism
homodimer 2 * 47000, SDS-PAGE Shewanella oneidensis
homodimer 2 * about 20000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
nicotinamide mononucleotide deamidase
-
 Escherichia coli
nicotinamide mononucleotide deamidase
-
 Shewanella oneidensis
NMN deamidase
-
 Escherichia coli
NMN deamidase
-
 Shewanella oneidensis
PncC
-
 Shewanella oneidensis
YgaD
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3
-
 nicotinamide mononucleotide in 100 mM potassium phosphate, pH 8.0, 10 mM sodium fluoride, 10 mM EDTA, at 37°C Escherichia coli
3.3
-
 nicotinamide mononucleotide in 100 mM potassium phosphate, pH 8.0, 10 mM sodium fluoride, 10 mM EDTA, at 37°C Shewanella oneidensis

pH Range

pH Minimum pH Maximum Comment Organism
5 10 the enzyme shows a broad optimum pH, ranging from 5.5 to 9.0, 72 and 60% residual activity is observed at pH 10.0 and 5.0, respectively Shewanella oneidensis

General Information

General Information Comment Organism
metabolism the only possible way of nicotinamide salvage in Shewanella oneidensis Shewanella oneidensis