Information on EC 3.4.23.24 - Candidapepsin

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.23.24
-
RECOMMENDED NAME
GeneOntology No.
Candidapepsin
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Preferential cleavage at the carboxyl of hydrophobic amino acids, but fails to cleave Leu15-Tyr, Tyr16-Leu and Phe24-Phe of insulin B chain. Activates trypsinogen, and degrades keratin
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
69458-91-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 11
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-
Manually annotated by BRENDA team
strain CBS 5982
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-
Manually annotated by BRENDA team
strain CBS-2730
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Manually annotated by BRENDA team
strain H12
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-
Manually annotated by BRENDA team
strains CD36 and CD92
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-
Manually annotated by BRENDA team
Candida olea
148
-
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Manually annotated by BRENDA team
Candida olea 148
148
-
-
Manually annotated by BRENDA team
strain CP386/IDE98 isolated from human ear and CP924/IDE03 isolated from human urine
UniProt
Manually annotated by BRENDA team
KSY 188-5
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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Sap1 to Sap6 do not play a significant role in Candida albicans virulence in a murine model of hematogenously disseminated candidiasis. In this model, Sap1 to Sap3 are not necessary for successful Candida albicans infection. A deficiency in SAP4 to SAP6 has no noticeable impact on the immune response elicited in the spleens and kidneys of Candida albicans-infected mice
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-(4-dimethylaminophenylazo)benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-[5-(2-aminoethyl)-amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu-Val-Ile-His + Thr-[5-(2-aminoethyl)-amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
-
-
-
?
4-(4-dimethylaminophenylazo)benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-[5-(2-aminoethyl)-amino]naphthalene-1-sulfonic acid + H2O
?
show the reaction diagram
-
-
-
-
?
4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys-Leu-Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys + Leu-Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid
show the reaction diagram
4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys-Leu-Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys-Leu + Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid
show the reaction diagram
4-(4-dimethylaminophenylazo)benzyl-Pro-Lys-Val-Glu-Leu-Thr-Gly-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzyl-Pro-Lys-Val-Glu + Leu-Thr-Gly-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid
show the reaction diagram
Ala-Thr-His-Gln-Val-Tyr-4-nitrophenylalanine-Val-Arg-Lys-Ala + H2O
Ala-Thr-His-Gln-Val-Tyr + 4-nitrophenylalanine-Val-Arg-Lys-Ala
show the reaction diagram
-
-
-
-
?
Albumin + H2O
?
show the reaction diagram
Arg-Glu-[5-(aminoethyl)aminonaphthalene sulfonate]-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys-[4'-dimethylaminoazobenzene-4-carboxylate]-Arg + H2O
?
show the reaction diagram
azocasein + H2O
?
show the reaction diagram
-
-
-
-
-
Azocoll + H2O
?
show the reaction diagram
-
-
-
-
-
Bovine serum albumin + H2O
?
show the reaction diagram
Bovine serum albumin + H2O
Hydrolyzed bovine serum albumin
show the reaction diagram
casein + H2O
?
show the reaction diagram
Collagen + H2O
?
show the reaction diagram
complement components C3b + H2O
?
show the reaction diagram
-
substrate for isoforms Sap1, Sap2, Sap3, but not for Sap9
-
-
?
complement components C4b + H2O
?
show the reaction diagram
-
substrate for isoforms Sap1, Sap2, Sap3, but not for Sap9
-
-
?
complement components C5 + H2O
?
show the reaction diagram
-
substrate for isoforms Sap1, Sap2, Sap3, but not for Sap9
-
-
?
Dabcyl-EHVKLVE-EDANS + H2O
?
show the reaction diagram
-
-
-
-
?
dabcyl-Glu-His-Val-Lys-Leu-Val-Glu-EDANS
dabcyl-Glu-His-Val-Lys-COOH + Leu-Val-Glu-EDANS
show the reaction diagram
dabcyl-Glu-His-Val-Lys-Leu-Val-Glu-EDANS + H2O
dabcyl-Glu-His-Val-Lys-Leu + Val-Glu-EDANS
show the reaction diagram
dabcyl-Pro-Lys-Val-Glu-Leu-Thr-Gly-Glu-EDANS
dabcyl-Pro-Lys-Val-Glu-COOH + Leu-Thr-Gly-Glu-EDANS
show the reaction diagram
E-cadherin + H2O
?
show the reaction diagram
-
Candida albicans strains JKC19 and SC5314 degrade E-cadherin at pH 4.0, the 10fold concentrated growth media of the strains HLC-52, HLC-54, 32723 and B1134 cause degradation at pH 4.0, strains HLC-52 and HLC-54 also at pH 6.0
-
-
?
ELSKRSSPS + H2O
ELSK + L-Arg + SSPS
show the reaction diagram
FRETS-25Ala + H2O
?
show the reaction diagram
-
isozyme Sap1 shows highest activity towards FRETS-25Ala
-
-
?
FRETS-25Xaa + H2O
?
show the reaction diagram
-
all Sap isozymes prefer Arg and Lys at the Xaa position
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-
?
Hemoglobin + H2O
?
show the reaction diagram
hide powder azure + H2O
?
show the reaction diagram
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-
-
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Human stratum corneum + H2O
?
show the reaction diagram
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-
-
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Keratin + H2O
?
show the reaction diagram
LPVNATSE + H2O
LPVN + ATSE
show the reaction diagram
LTEKRDSIS + H2O
LTEKR + L-Asp + Ser-Ile-Ser
show the reaction diagram
Lys-Pro-Ala-Arg-Phe-Nph-Arg-Leu + H2O
?
show the reaction diagram
-
-
-
-
?
Lys-Pro-Ala-Arg-Phe-Nph-Arg-Leu + H2O
Lys-Pro-Ala-Arg-Phe + Nph-Arg-Leu
show the reaction diagram
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-
-
-
?
Lys-Pro-Ala-Glu-Phe-4-nitrophenylalanine-Ala-Leu + H2O
Lys-Pro-Ala-Glu-Phe + 4-nitrophenylalanine-Ala-Leu
show the reaction diagram
-
-
-
-
?
Lys-Pro-Leu-Glu-Met-4-nitrophenylalanine-Ala-Leu + H2O
Lys-Pro-Leu-Glu-Met + 4-nitrophenylalanine-Ala-Leu
show the reaction diagram
-
-
-
-
?
Myeloma proteins of the type A1 and A2 + H2O
?
show the reaction diagram
oxidized insulin B chain + H2O
?
show the reaction diagram
-
cleavage sites of Sap2p: FV-/-NQHLCGSHL-/-V-/-EA-/-LYLVCGERGFF-/-YTPKA
-
-
?
Oxidized insulin B-chain + H2O
?
show the reaction diagram
-
low side-chain specificity, preferential attack on hydrophobic amino acid residues
-
-
-
PMVELAGE + H2O
PMVE + L-Leu + AGE
show the reaction diagram
PMVELAGE + H2O
PMVEL + AGE
show the reaction diagram
100% conversion
-
-
?
PMVELGGE + H2O
PMVEL + GGE
show the reaction diagram
100% conversion
-
-
?
PMVELHGE + H2O
PMVEL + HGE
show the reaction diagram
100% conversion
-
-
?
PMVELPGE + H2O
PMVEL + PGE
show the reaction diagram
100% conversion
-
-
?
PMVELQGE + H2O
PMVE + L-Leu + QGE
show the reaction diagram
18% conversion
-
-
?
PMVELQGE + H2O
PMVEL + QGE
show the reaction diagram
100% conversion
-
-
?
PMVELTGE + H2O
PMVE + L-Leu + Tyr-Gly-Glu
show the reaction diagram
35% conversion
-
-
?
PMVELTGE + H2O
PMVEL + Tyr-Gly-Glu
show the reaction diagram
100% conversion
-
-
?
PMVELWGE + H2O
PMVE + L-Leu + WGE
show the reaction diagram
40% conversion
-
-
?
PMVELWGE + H2O
PMVEL + WGE
show the reaction diagram
76% conversion
-
-
?
PMVEMWGE + H2O
PMVEM + WGE
show the reaction diagram
100% conversion
-
-
?
PMVMLWGE + H2O
PMVML + WGE
show the reaction diagram
protein + H2O
hydrolyzed protein
show the reaction diagram
Proteinase inhibitors of human serum + H2O
?
show the reaction diagram
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-
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
protein + H2O
hydrolyzed protein
show the reaction diagram
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,5R,7R)-3-benzyl-7-(piperidin-1-ylcarbonyl)-6,8-dioxa-3-azabicyclo[3.2.1]octan-2-one
-
74% inhibition at 0.02 mM
(1R,5R,7R)-3-benzyl-N-(2-hydroxyethyl)-2-oxo-6,8-dioxa-3-azabicyclo[3.2.1]octane-7-carboxamide
-
71% inhibition at 0.02 mM
(1R,5R,7R)-3-benzyl-N-(3-hydroxypropyl)-2-oxo-6,8-dioxa-3-azabicyclo[3.2.1]octane-7-carboxamide
-
23% inhibition at 0.02 mM
(1R,5R,7R)-3-benzyl-N-[(1R)-2-hydroxy-1-phenylethyl]-2-oxo-6,8-dioxa-3-azabicyclo[3.2.1]octane-7-carboxamide
-
31% inhibition at 0.02 mM
(1R,5R,7R)-3-benzyl-N-[(2R)-1-hydroxy-3-phenylpropan-2-yl]-2-oxo-6,8-dioxa-3-azabicyclo[3.2.1]octane-7-carboxamide
-
29% inhibition at 0.02 mM
(1R,5R,7R)-3-benzyl-N-[(2S)-1-hydroxy-3-phenylpropan-2-yl]-2-oxo-6,8-dioxa-3-azabicyclo[3.2.1]octane-7-carboxamide
-
30% inhibition at 0.02 mM
(1R,5R,7R)-3-benzyl-N-[(2S)-1-hydroxy-4-methylpentan-2-yl]-2-oxo-6,8-dioxa-3-azabicyclo[3.2.1]octane-7-carboxamide
-
71% inhibition at 0.02 mM
(1R,5R,7R)-3-benzyl-N-[(2S)-1-hydroxypropan-2-yl]-2-oxo-6,8-dioxa-3-azabicyclo[3.2.1]octane-7-carboxamide
-
35% inhibition at 0.02 mM
(1R,5R,7R)-N-(2-aminoethyl)-3-benzyl-2-oxo-6,8-dioxa-3-azabicyclo[3.2.1]octane-7-carboxamide
-
63% inhibition at 0.02 mM
(1R,5S,7R)-3-benzyl-7-(1,4-diazepan-1-ylcarbonyl)-6,8-dioxa-3-azabicyclo[3.2.1]octan-2-one
-
63% inhibition at 0.02 mM
(1R,5S,7R)-3-benzyl-7-(morpholin-4-ylcarbonyl)-6,8-dioxa-3-azabicyclo[3.2.1]octan-2-one
-
62% inhibition at 0.02 mM
(1R,5S,7R)-3-benzyl-7-(thiomorpholin-4-ylcarbonyl)-6,8-dioxa-3-azabicyclo[3.2.1]octan-2-one
-
58% inhibition at 0.02 mM
(1R,5S,7R)-3-benzyl-7-[[(2R)-2-(hydroxymethyl)pyrrolidin-1-yl]carbonyl]-6,8-dioxa-3-azabicyclo[3.2.1]octan-2-one
-
34% inhibition at 0.02 mM
(1R,5S,7R)-3-benzyl-7-[[(2S)-2-(hydroxymethyl)pyrrolidin-1-yl]carbonyl]-6,8-dioxa-3-azabicyclo[3.2.1]octan-2-one
-
48% inhibition at 0.02 mM
(1R,5S,7R)-3-benzyl-7-[[4-(2-hydroxyethyl)piperazin-1-yl]carbonyl]-6,8-dioxa-3-azabicyclo[3.2.1]octan-2-one
-
58% inhibition at 0.02 mM
(1R,5S,7S)-3-benzyl-2-oxo-6,8-dioxa-3-azabicyclo[3.2.1]octane-7-carboxylic acid [1-hydroxymethyl-3(R)-methylbutyl]amide
-
60% inhibition at 0.02 mM
(1R,5S,7S)-3-benzyl-2-oxo-6,8-dioxa-3-azabicyclo[3.2.1]octane-7-carboxylic acid [1-hydroxymethyl-3(S)-methylbutyl]amide
-
33% inhibition at 0.02 mM
(1S,5R,7R)-3-benzyl-2-oxo-6,8-dioxa-3-azabicyclo[3.2.1]octane-7-carboxylic acid benzyl-(1-hydroxymethyl-3-methylbutyl)amide
-
35% inhibition at 0.02 mM
(1S,5R,7R)-3-benzyl-2-oxo-6,8-dioxa-3-azabicyclo[3.2.1]octane-7-carboxylic acid [1-hydroxymethyl-3(R)-methylbutyl]amide
-
29% inhibition at 0.02 mM
(1S,5S,7S)-3-benzyl-7-(piperidine-1-carbonyl)-6,8-dioxa-3-azabicyclo[3.2.1]octan-2-one
-
50% inhibition at 0.02 mM
(2R,3S)-phenylnorstatine
(2S,3S)-phenylnorstatine
(3S,4S)-phenylstatine
(3S,4S)-statine
1,2-epoxy-3-(4-nitrophenoxy)propane
acetyl-Phe-Ile-Phe-psi[OH-OH]-Phe-Glu-Phe-acetyl
-
transition-state peptidomimetic TS-41
acetyl-Trp-Phe-psi[OH-OH]-Phe-Trp-acetyl
-
transition-state peptidomimetic TS-49
acetyl-Trp-Ser-Phe-psi[OH-OH]-Phe-kynurenic acid
-
transition-state peptidomimetic TS-57
acetyl-Trp-Val-Phe-psi[OH-OH]-Phe-Glu-Phe-acetyl
-
transition-state peptidomimetic TS-43
acetyl-Trp-Val-Phe-psi[OH-OH]-Phe-Val-acetyl
-
transition-state peptidomimetic TS-75
acetyl-Trp-Val-Phe-psi[OH-OH]-Phe-Val-Trp-acetyl
-
transition-state peptidomimetic TS-42
amprenavir
-
most effective inhibitor of Sap2, about 92% inhibition at 0.1 mM
antipain
-
partial
Aprotinin
-
-
Arg-Ile-Phe-psi[CH2-NH]-Phe-Gln-Arg
-
transition-state peptidomimetic TS-2
benzyloxycarbonyl-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe
;
benzyloxycarbonyl-L-Val-L-Val-((3R,4R)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe
;
benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-( (3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe
;
benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3R,4R)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe
;
benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-NH2
;
benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OH
;
benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-OMe
;
benzyloxycarbonyl-L-Val-L-Val-statine-L-Ala-statine-OH
;
Butanedione
-
-
chymostatin
-
-
Diazoacetylnorleucine methyl ester
ethyl 4-[[(1R,5S,7R)-3-benzyl-2-oxo-6,8-dioxa-3-azabicyclo[3.2.1]oct-7-yl]carbonyl]piperazine-1-carboxylate
-
74% inhibition at 0.02 mM
fluconazole
-
-
indinavir
-
-
inhibitor A-70450
-
isovaleryl-Val-Val-statyl-Ala-statyl-OH
-
-
kynurenic acid-Dtg-Phe-psi(S,R,S)[OH]-Phe-dimethylphenoxyacetic acid
-
transition-state peptidomimetic TS-92
kynurenic acid-Dtg-Phe-psi[OH-OH]-Phe-phenoxyacetic acid
-
transition-state peptidomimetic TS-63
kynurenic acid-Thr-Phe-psi(S,R,S)[OH]-Phe-dimethylphenoxyacetic acid
-
transition-state peptidomimetic TS-93
kynurenic acid-Thr-Phe-psi[OH-OH]-Phe-kynurenic acid
-
transition-state peptidomimetic TS-59
kynurenic acid-Val-Phe-psi(S,R,S)[OH]-Phe-dimethylphenoxyacetic acid
-
transition-state peptidomimetic TS-94
kynurenic acid-Val-Phe-psi(S,R,S)[OH]-Phe-Val-kynurenic acid
-
transition-state peptidomimetic TS-91
kynurenic acid-Val-Phe-psi[OH-OH]-Phe-Val-kynurenic acid
-
best inhibitor; transition-state peptidomimetic TS-70
lopinavir
-
-
Lys-Ile-Phe-psi[CH2-NH]-Phe-Gln-Arg
-
transition-state peptidomimetic TS-23
nelfinavir
-
-
Pepstatin
pepstatin A
ritonavir
saquinavir
SDS
-
tolerated at 0.01% w/v, decrease of activity at 0.1% w/v, 1% completely destroys activity within a few min
squinavir
-
;
t-butoxylcarbonyl-Phe-psi[OH-OH]-Phe-Dtg-phenoxyacetic acid
-
transition-state peptidomimetic TS-54
t-butoxylcarbonyl-Phe-psi[OH-OH]-Phe-Glu-Phe-acetyl
-
transition-state peptidomimetic TS-53
tert-butoxycarbonyl-Val-Val-(3R,4R)-4-amino-3-hydroxy-5-phenylpentanoyl-Ala-phenylstatyl-O-methyl ester
-
-
tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-(3R,4R)-4-amino-3-hydroxy-5-phenylpentanoyl-OH
-
-
tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-O-methyl ester
-
-
tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-phenylstatyl-NH2
-
-
tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-phenylstatyl-O-methyl ester
-
-
tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-phenylstatyl-OH
-
-
Trp-Ile-Phe-psi[CH2-NH]-Phe-Gln-Trp
-
transition-state peptidomimetic TS-10
Xan-Dtg-Val-psi(S,R,S)[OH]-Val-Dtg-Xan
-
transition-state peptidomimetic TS-90
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
fluconazole
enhances expression of SAP4-GFP and SAP6-GFP and reduces growth of Candida albicans to 60% to 80% at 0.5 mg/l; enhances expression of SAP4-GFP and SAP6-GFP and reduces growth of Candida albicans to 60% to 80% at 0.5 mg/l, respectively; enhances expression of SAP4-GFP and SAP6-GFP and reduces growth of Candida albicans to 60% to 80% at 0.5 mg/l, respectively
Nonionic detergents
-
Triton X-100, Tween-80, Nonidet P-40, slight improvement of activity
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.3
4-(4-dimethylaminophenylazo)benzoyl-gamma-aminobutyryl-Ile-HIs-Pro-Phe-His-Leu-Val-Ile-His-Thr-[5-(2-aminoethyl)-amino]naphthalene-1-sulfonic acid
-
isoenzyme Sap2p; isoenzyme Sap2p
0.00113 - 0.0028
4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys-Leu-Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid
0.0122
Ala-Thr-His-Gln-Val-Tyr-4-nitrophenylalanine-Val-Arg-Lys-Ala
-
-
0.07
albumin
-
-
-
0.008
Lys-Pro-Ala-Arg-Phe-Nph-Arg-Leu
-
pH 3.25, isoenzyme Sap2p; pH 3.25, isoenzyme Sap2p
0.0092
Lys-Pro-Ala-Glu-Phe-4-nitrophenylalanine-Ala-Leu
-
-
0.0129
Lys-Pro-Leu-Glu-Met-4-nitrophenylalanine-Ala-Leu
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
15.2 - 48.7
4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys-Leu-Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid
1.41
Ala-Thr-His-Gln-Val-Tyr-4-nitrophenylalanine-Val-Arg-Lys-Ala
Candida parapsilosis
-
-
31.8
Lys-Pro-Ala-Glu-Phe-4-nitrophenylalanine-Ala-Leu
Candida parapsilosis
-
-
24.11
Lys-Pro-Leu-Glu-Met-4-nitrophenylalanine-Ala-Leu
Candida parapsilosis
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000143 - 0.000044
benzyloxycarbonyl-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe
0.000174 - 0.002782
benzyloxycarbonyl-L-Val-L-Val-((3R,4R)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe
0.0000002 - 0.0000003
benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-( (3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe
0.0000001 - 0.0000004
benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3R,4R)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe
0.0000001
benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-NH2
isoform Sapp1p, in 150 mM sodium citrate buffer, pH 3.75, at 37C; isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37C
0.0000018 - 0.0000066
benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OH
0.0000072 - 0.0000146
benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-OMe
0.00000003 - 0.0000003
benzyloxycarbonyl-L-Val-L-Val-statine-L-Ala-statine-OH
0.0000003
isovaleryl-Val-Val-statyl-Ala-statyl-OH
-
-
0.0000003 - 0.017
pepstatin A
0.0003 - 0.0019
ritonavir
0.0169 - 0.32
saquinavir
0.0068
squinavir
-
;
0.005301
tert-butoxycarbonyl-Val-Val-(3R,4R)-4-amino-3-hydroxy-5-phenylpentanoyl-Ala-phenylstatyl-O-methyl ester
-
-
0.0000009
tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-(3R,4R)-4-amino-3-hydroxy-5-phenylpentanoyl-OH
-
-
0.0000065
tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-O-methyl ester
-
-
0.00000254
tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-phenylstatyl-NH2
-
-
0.0000004
tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-phenylstatyl-O-methyl ester
-
-
0.0000066
tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-phenylstatyl-OH
-
-
additional information
additional information
-
second order inhibition rate constants between 56.000 and 121.000M(-1)min(-1) for cis-configured epoxides and aziridines
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02
(1S,5S,7S)-3-benzyl-7-(piperidine-1-carbonyl)-6,8-dioxa-3-azabicyclo[3.2.1]octan-2-one
Candida albicans
-
in 50 mM sodium citrate, pH 3.2, at 37C
0.000027
pepstatin A
Candida albicans
-
IC50 for sap2p: 27 nM; IC50 for sap2p: 27 nM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.5 - 3.9
-
hemoglobin
2.5
-
isozyme Sap8
3
-
isozyme Sap3
3.3
Candida olea
-
denatured hemoglobin
3.7
-
bovine serum albumin
4 - 5.5
-
chaotropic agents such as KSCN shift the pH optimum to 6.5
4.5
-
isoenzyme Sap2p, hydrolysis of 4-(4-dimethylaminophenylazo)benzoyl-gamma-aminobutyryl-Ile-HIs-Pro-Phe-His-Leu-Val-Ile-His-Thr-[5-(2-aminoethyl)-amino]naphthalene-1-sulfonic acid; isoenzyme Sap2p, hydrolysis of 4-(4-dimethylaminophenylazo)benzoyl-gamma-aminobutyryl-Ile-HIs-Pro-Phe-His-Leu-Val-Ile-His-Thr-[5-(2-aminoethyl)-amino]naphthalene-1-sulfonic acid
5
-
isozyme Sap1; isozyme Sap4; isozyme Sap5; isozyme Sap6
5.5
-
isozyme Sap9
6
-
isozyme Sap10
6.5
-
isozyme Sap7
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.5 - 6
-
inactive below and above
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42
Candida olea
-
denatured hemoglobin
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 42
Candida olea
-
0C: 5-10% of activity maximum, 42C: activity maximum
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
biofilms of Candida albicans secrete more SAPs than the planktonic form
Manually annotated by BRENDA team
-
biofilms of Candida albicans secrete more SAPs than the planktonic form
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
the enzyme is temporarily associated with the cell wall
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11000
-
1 * 44000 + 1 * 11000, isozyme Sap9, SDS-PAGE; 1 * 44000 + 1 * 11000, isozyme Sap9, SDS-PAGE
15000
-
1 * 52000 + 1 * 15000, isozyme Sap7, SDS-PAGE; 1 * 52000 + 1 * 15000, isozyme Sap7, SDS-PAGE
29500
Candida olea
-
1 * 29500, Candida olea, SDS-PAGE
30900
Candida olea
-
Candida olea, gel filtration
35000
-
isozyme Sap8, calculated from amino acid sequence
35500
-
x * 35500, recombhinant truncated Sap2 protein, SDS-PAGE
36500
-
1 * 36500, Candida pulcherrima, SDS-PAGE
37000
-
isozyme Sap3, SDS-PAGE and calculated from amino acid sequence; isozyme Sap4, calculated from amino acid sequence; isozyme Sap5, calculated from amino acid sequence; isozyme Sap6, calculated from amino acid sequence
39000
-
isozyme Sap1, estimated from SDS-PAGE; isozyme Sap4, estimated from SDS-PAGE
47000
-
isozyme Sap7, calculated from amino acid sequence
52000
-
1 * 52000 + 1 * 15000, isozyme Sap7, SDS-PAGE; isozyme Sap7, estimated from SDS-PAGE
53000
-
isozyme Sap9, calculated from amino acid sequence
55000 - 60000
-
Candida albicans, proteinase A, gel filtration
86000
-
Candida albicans
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
-
1 * 44000 + 1 * 11000, isozyme Sap9, SDS-PAGE; 1 * 44000 + 1 * 11000, isozyme Sap9, SDS-PAGE; 1 * 52000 + 1 * 15000, isozyme Sap7, SDS-PAGE; 1 * 52000 + 1 * 15000, isozyme Sap7, SDS-PAGE
monomer
tetramer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
proteolytic modification
-
the Sapp1 precursor can be processed autocatalytically and by a membrane-bound processing proteinase. The pro-segment is indispensable for Sapp1p to attain an appropriate structure
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
complexed with inhibitor A-70450
of inhibited enzyme
-
Sap2p-inhibitor complex solved to 2.1 A
-
Sap3 with PepA in the presence of 10 mM zinc acetate, Sap3 without PepA in the presence of 0.2M potassium bromide, 15% PEG4000 and 0.1 M cacodylic acid/NaOH, pH 6.5. The Sap3 protein crystallizes in the trigonal space group P3(2)21 containing one molecule per asymmetric unit.
-
X-ray crystal structures of Sap1 and Sap5; X-ray crystal structures of Sap1 and Sap5
Sapp1p in complex with pepstatin A, hanging drop vapor diffusion method, using 0.1 M Tris-HCl, pH 7.0, 2.0 M ammonium sulfate and 10% (v/v) glycerol, at 19C
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 5
-
37C, 12 h, maximal stability
30691
6
-
unstable above
30694
6.5
-
extremely unstable above
30691
8
Candida olea
-
10 min, over 70% loss of activity
30693
8.4
-
above, alkaline denaturation accompanied by dimerization
30683
9
Candida olea
-
10 min, complete loss of activity
30693
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
Candida olea
-
pH 3.2, 60 min, slight loss of activity
37
-
10 min, 85% loss of activity at pH 7.5, 5% loss of activity at pH 6.6 and 7.0
46
Candida olea
-
inactivation above
50
-
stable at
60
Candida olea
-
almost 80% loss of activity after 10 min, complete loss of activity after 20 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Enzyme gradually loses activity by standing in solution in the cold or in the frozen state
-
Stable in presence of non-ionic detergents
-
Stable to freeze-drying
-
Unstable to lyophilization
-
unstable to repeated freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0C, stable for at least 2 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, MonoS column chromatography, and MonoP column chromatography
-
anion exchange chromatography
-
anti-FLAG M2 affinity gel filtration; anti-FLAG M2 affinity gel filtration; anti-FLAG M2 affinity gel filtration; anti-FLAG M2 affinity gel filtration; anti-FLAG M2 affinity gel filtration
-
from the culture medium of a virulent strain
-
MonoS column chromatography
Ni-NTA column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Candida albicans ATCC 10231 and ATCC 10261, contain two genes for a secreted aspartate proteinase, PRA10 and PRA11, cloned and expressed in Escherichia coli TG1 and DH5alpha
-
expressed in Escherichia coli M15 cells
-
expressed in Escherichia coli Top-10 cells
expressed in Pichia pastoris
-
expressed in Pichia pastoris strain GS115; expressed in Pichia pastoris strain GS115; expressed in Pichia pastoris strain GS115; expressed in Pichia pastoris strain GS115; expressed in Pichia pastoris strain GS115
-
expression in Escherichia coli
-
expression in Pichia pastoris
-
expression of the Sapp2p precursor in E.coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of SAPP1 is induced only by the presence of exogenous protein as the sole nitrogen source
for good Sap-inducing conditions, cells are incubated in 1.2% (w/v) yeast carbon base medium supplemented with 0.1% (w/v) bovine serum albumin, pH 5.0, at 37C for 48 h under slight agitation (100 rpm)
-
isoforms SAP5 and SAP6 mRNA are expressed higher in Candida albicans strain SC5314 than strain VE175
-
SAPT2-4 transcripts are frequently detected 12 h after infection of reconstituted human oral epithelium
-
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
if the SAP1 gene having a deletion of the propeptide is expressed in Pichia pastoris, the protein accumulates in the cell and fails to be secreted
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine