Literature summary for 3.4.23.24 extracted from

Hruskova-Heidingsfeldova, O.; Dostal, J.; Majer, F.; Havlikova, J.; Hradilek, M.; Pichova, I.
Two aspartic proteinases secreted by the pathogenic yeast Candida parapsilosis differ in expression pattern and catalytic properties (2009), Biol. Chem., 390, 259-268.

Search for more literature for 3.4.23.24

Inhibitors

Inhibitors	Comment	Organism
benzyloxycarbonyl-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe	-	Candida parapsilosis
benzyloxycarbonyl-L-Val-L-Val-((3R,4R)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe	-	Candida parapsilosis
benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-( (3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe	-	Candida parapsilosis
benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3R,4R)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe	-	Candida parapsilosis
benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-NH2	-	Candida parapsilosis
benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OH	-	Candida parapsilosis
benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-OMe	-	Candida parapsilosis
benzyloxycarbonyl-L-Val-L-Val-statine-L-Ala-statine-OH	-	Candida parapsilosis
additional information	not inhibited by indinavir and nelfinavir; not inhibited by indinavir and nelfinavir	Candida parapsilosis
pepstatin A	-	Candida parapsilosis
ritonavir	-	Candida parapsilosis
saquinavir	-	Candida parapsilosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00113	-	4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys-Leu-Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid	isoform Sapp1p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0028	-	4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys-Leu-Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid	isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis

Organism

Organism	UniProt	Comment	Textmining
Candida parapsilosis	P32950	-	-
Candida parapsilosis	P32951	-	-
Candida parapsilosis P-69	P32950	-	-
Candida parapsilosis P-69	P32951	-	-

Purification (Commentary)

Purification (Comment)	Organism
MonoS column chromatography	Candida parapsilosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys-Leu-Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid + H2O	Sapp2p preferentially cleaves the Lys-Leu bond	Candida parapsilosis	4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys + Leu-Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid	-	?
4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys-Leu-Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid + H2O	Sapp2p preferentially cleaves the Lys-Leu bond	Candida parapsilosis P-69	4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys + Leu-Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid	-	?
4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys-Leu-Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid + H2O	Sapp1p preferentially cleaves the Leu-Val bond	Candida parapsilosis	4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys-Leu + Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid	-	?
4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys-Leu-Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid + H2O	Sapp1p preferentially cleaves the Leu-Val bond	Candida parapsilosis P-69	4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys-Leu + Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid	-	?
4-(4-dimethylaminophenylazo)benzyl-Pro-Lys-Val-Glu-Leu-Thr-Gly-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid + H2O	the substrate is hydrolyzed preferentially at the Glu-Leu bond, however, when reaction times are prolonged, additional cleavage occurs at the Lys-Val position	Candida parapsilosis	4-(4-dimethylaminophenylazo)benzyl-Pro-Lys-Val-Glu + Leu-Thr-Gly-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid	-	?
4-(4-dimethylaminophenylazo)benzyl-Pro-Lys-Val-Glu-Leu-Thr-Gly-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid + H2O	the substrate is hydrolyzed preferentially at the Glu-Leu bond, however, when reaction times are prolonged, additional cleavage occurs at the Lys-Val position	Candida parapsilosis P-69	4-(4-dimethylaminophenylazo)benzyl-Pro-Lys-Val-Glu + Leu-Thr-Gly-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid	-	?
ELSKRSSPS + H2O	98% conversion	Candida parapsilosis	ELSK + L-Arg + SSPS	-	?
ELSKRSSPS + H2O	98% conversion	Candida parapsilosis P-69	ELSK + L-Arg + SSPS	-	?
LPVNATSE + H2O	28% conversion	Candida parapsilosis	LPVN + ATSE	-	?
LPVNATSE + H2O	28% conversion	Candida parapsilosis P-69	LPVN + ATSE	-	?
LTEKRDSIS + H2O	100% conversion	Candida parapsilosis	LTEKR + L-Asp + Ser-Ile-Ser	-	?
LTEKRDSIS + H2O	100% conversion	Candida parapsilosis P-69	LTEKR + L-Asp + Ser-Ile-Ser	-	?
additional information	isoform Sapp2p has a more restricted substrate specificity and significantly lower catalytic activity than isoform Sapp1p	Candida parapsilosis	?	-	?
additional information	no hydrolysis of PMVELPGE, PMVELHGE, PMVELGGE, PMVEMWGE, LPVNATSE, LTEKRDSIS, and ELSKRSSPS	Candida parapsilosis	?	-	?
additional information	isoform Sapp2p has a more restricted substrate specificity and significantly lower catalytic activity than isoform Sapp1p	Candida parapsilosis P-69	?	-	?
additional information	no hydrolysis of PMVELPGE, PMVELHGE, PMVELGGE, PMVEMWGE, LPVNATSE, LTEKRDSIS, and ELSKRSSPS	Candida parapsilosis P-69	?	-	?
PMVELAGE + H2O	cleavage of Sapp2p, 81% conversion	Candida parapsilosis	PMVE + L-Leu + AGE	-	?
PMVELAGE + H2O	cleavage of Sapp2p, 81% conversion	Candida parapsilosis P-69	PMVE + L-Leu + AGE	-	?
PMVELAGE + H2O	cleavage of Sapp1p, 100% conversion	Candida parapsilosis	PMVEL + AGE	-	?
PMVELGGE + H2O	100% conversion	Candida parapsilosis	PMVEL + GGE	-	?
PMVELHGE + H2O	100% conversion	Candida parapsilosis	PMVEL + HGE	-	?
PMVELPGE + H2O	100% conversion	Candida parapsilosis	PMVEL + PGE	-	?
PMVELQGE + H2O	cleavage of Sapp2p, 18% conversion	Candida parapsilosis	PMVE + L-Leu + QGE	-	?
PMVELQGE + H2O	cleavage of Sapp1p, 100% conversion	Candida parapsilosis	PMVEL + QGE	-	?
PMVELTGE + 2 H2O	cleavage of Sapp2p, 35% conversion	Candida parapsilosis	PMVE + L-Leu + TGE	-	?
PMVELTGE + H2O	cleavage of Sapp1p, 100% conversion	Candida parapsilosis	PMVEL + TGE	-	?
PMVELWGE + H2O	cleavage of Sapp2p, 40% conversion	Candida parapsilosis	PMVE + L-Leu + WGE	-	?
PMVELWGE + H2O	cleavage of Sapp1p, 76% conversion	Candida parapsilosis	PMVEL + WGE	-	?
PMVEMWGE + H2O	100% conversion	Candida parapsilosis	PMVEM + WGE	-	?
PMVMLWGE + H2O	13% conversion	Candida parapsilosis	PMVML + WGE	-	?
PMVMLWGE + H2O	98% conversion	Candida parapsilosis	PMVML + WGE	-	?

Synonyms

Synonyms	Comment	Organism
Candidapepsin-1	-	Candida parapsilosis
candidapepsin-2	-	Candida parapsilosis
Sapp1p	isoform	Candida parapsilosis
Sapp2p	isoform	Candida parapsilosis
secreted aspartic proteinase	-	Candida parapsilosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
15.2	-	4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys-Leu-Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid	isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
48.7	-	4-(4-dimethylaminophenylazo)benzyl-Glu-His-Val-Lys-Leu-Val-Glu-5-(2-aminoethylamino)-1-naphthalenesulfonic acid	isoform Sapp1p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.00000003	-	benzyloxycarbonyl-L-Val-L-Val-statine-L-Ala-statine-OH	isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0000001	-	benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-NH2	isoform Sapp1p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0000001	-	benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-NH2	isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0000001	-	benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3R,4R)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe	isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0000002	-	benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-( (3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe	isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0000003	-	pepstatin A	isoform Sapp1p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0000003	-	benzyloxycarbonyl-L-Val-L-Val-statine-L-Ala-statine-OH	isoform Sapp1p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0000003	-	benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-( (3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe	isoform Sapp1p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0000004	-	benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3R,4R)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe	isoform Sapp1p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0000004	-	pepstatin A	isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0000018	-	benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OH	isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0000066	-	benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OH	isoform Sapp1p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0000072	-	benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-OMe	isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0000143	-	benzyloxycarbonyl-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe	isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0000146	-	benzyloxycarbonyl-L-Val-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-OMe	isoform Sapp1p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.000044	-	benzyloxycarbonyl-L-Val-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe	isoform Sapp1p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.000174	-	benzyloxycarbonyl-L-Val-L-Val-((3R,4R)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe	isoform Sapp1p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0003	-	ritonavir	isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0019	-	ritonavir	isoform Sapp1p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.002782	-	benzyloxycarbonyl-L-Val-L-Val-((3R,4R)-4-amino-3-hydroxy-5-phenylpentanoic acid)-L-Ala-((3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid)-OMe	isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.0169	-	saquinavir	isoform Sapp1p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis
0.32	-	saquinavir	isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C	Candida parapsilosis

Expression

Organism	Comment	Expression
Candida parapsilosis	expression of SAPP1 is induced only by the presence of exogenous protein as the sole nitrogen source	up