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Literature summary for 3.4.23.24 extracted from
- Candidapepsin (2004), Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press, 1, 115-120.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| Sap2p-inhibitor complex solved to 2.1 A | Candida albicans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| pepstatin A | IC50 for sap2p: 27 nM; IC50 for sap2p: 27 nM | Candida albicans |  |
| ritonavir | - |
Candida albicans | |
| squinavir | - |
Candida albicans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
Lys-Pro-Ala-Arg-Phe-Nph-Arg-Leu | pH 3.25, isoenzyme Sap2p | Candida albicans | |
| 4.3 | - |
4-(4-dimethylaminophenylazo)benzoyl-gamma-aminobutyryl-Ile-HIs-Pro-Phe-His-Leu-Val-Ile-His-Thr-[5-(2-aminoethyl)-amino]naphthalene-1-sulfonic acid | isoenzyme Sap2p | Candida albicans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Candida albicans | candidapepsin is implicated in the degradation of host proteins involved in defense and barrier function | ? | - |
? | |
| additional information | Candida albicans | candidapepsin is implicated in the degradation of host proteins involved in defense and barrier function. Sap2p degrades the Fc portion of IgG, IgA, complement factor C3 and endogenous protease inhibitors in human serum | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | P0CY27 | - |
- |
| Candida albicans | P0DJ06 | - |
- |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| if the SAP1 gene having a deletion of the propeptide is expressed in Pichia pastoris, the protein accumulates in the cell and fails to be secreted | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-(4-dimethylaminophenylazo)benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-[5-(2-aminoethyl)-amino]naphthalene-1-sulfonic acid + H2O | - |
Candida albicans | 4-(4-dimethylaminophenylazo)benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu-Val-Ile-His + Thr-[5-(2-aminoethyl)-amino]naphthalene-1-sulfonic acid | - |
? | |
| 4-(4-dimethylaminophenylazo)benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-[5-(2-aminoethyl)-amino]naphthalene-1-sulfonic acid + H2O | - |
Candida albicans | ? | - |
? | |
| Albumin + H2O | cleavage by Sap2p shows a low side-chain specificity | Candida albicans | ? | - |
? | |
| Lys-Pro-Ala-Arg-Phe-Nph-Arg-Leu + H2O | - |
Candida albicans | ? | - |
? | |
| Lys-Pro-Ala-Arg-Phe-Nph-Arg-Leu + H2O | - |
Candida albicans | Lys-Pro-Ala-Arg-Phe + Nph-Arg-Leu | - |
? | |
| additional information | candidapepsin is implicated in the degradation of host proteins involved in defense and barrier function | Candida albicans | ? | - |
? | |
| additional information | candidapepsin is implicated in the degradation of host proteins involved in defense and barrier function. Sap2p degrades the Fc portion of IgG, IgA, complement factor C3 and endogenous protease inhibitors in human serum | Candida albicans | ? | - |
? | |
| additional information | extremely broad specificity range | Candida albicans | ? | - |
? | |
| oxidized insulin B chain + H2O | cleavage sites of Sap2p: FV-/-NQHLCGSHL-/-V-/-EA-/-LYLVCGERGFF-/-YTPKA | Candida albicans | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| candialbicin | - |
Candida albicans |
| SAP11 | - |
Candida albicans |
| Sap2p | - |
Candida albicans |
| secreted aspartic proteinase 1 | - |
Candida albicans |
| secreted aspartic proteinase 2 | - |
Candida albicans |
| secretory aspartyl proteinase SAP1 | - |
Candida albicans |
| secretory aspartyl proteinase SAP2p | - |
Candida albicans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
isoenzyme Sap2p, hydrolysis of 4-(4-dimethylaminophenylazo)benzoyl-gamma-aminobutyryl-Ile-HIs-Pro-Phe-His-Leu-Val-Ile-His-Thr-[5-(2-aminoethyl)-amino]naphthalene-1-sulfonic acid | Candida albicans |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0003 | - |
ritonavir | - |
Candida albicans | |
| 0.0068 | - |
squinavir | - |
Candida albicans | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.000027 | - |
IC50 for sap2p: 27 nM | Candida albicans | pepstatin A | |
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