another cysteine endopeptidase, with similar action on small molecule substrates, pinguinain (formerly EC 22.214.171.124) is obtained from the related plant, Bromelia pinguin, but pinguinain differs from fruit bromelain in being inhibited by chicken cystatin
another cysteine endopeptidase, with similar action on small molecule substrates, pinguinain (formerly EC 126.96.36.199), is obtained from the related plant, Bromelia pinguin, but pinguinain differs from fruit bromelain in being inhibited by chicken cystatin
enzyme is assayed using casein as a substrate with Coomassie dye to track completion of hydrolysis of casein, casein is hydrolysed to completion leading to a brown coloured solution, assay method development as a Bradford dye-binding-bromelain-casein system as an assay for heavy metals, based on colour development and its inhibition by Hg2+ and Cu2+
an extended AE-rich N-terminal trunk in secreted pineapple cystatin enhances inhibition of fruit bromelain and is posttranslationally removed during ripening, The AE-rich N-terminal trunk is required to inhibit fruit bromelain (above 95%), whereas its removal decreases inhibition to 20%. Recombinant cystatin containing the complete AE-rich N-terminal trunk and recombinant medium pineapple cystatin effectively inhibit bromelain compared to recombinant core pineapple cystatin
bromelain is a crude extract containing a complex mixture of several components, such as different thiol-endopeptidases, phosphatases, glucosidases, peroxidases, cellulases, glycoproteins and carbohydrates, as well as several proteinase inhibitors
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incubation with trypsin (1 mg/ml, 1 h, 37░C) results in 12% loss of activity (with benzyloxycarbonyl-Arg-Arg-p-nitroanilide as substrate) at bromelain concentration of 10 mg/ml , but has no effect on activity at bromelain concentrations of 100 mg/ml
proteolytic activity of solutions containing either 10 mg/ml or 100 mg/ml bromelain in water or 100 mg/ml NaHCO3 is not changed against benzyloxycarbonyl-Arg-Arg-p-nitroanilide and benzoyl-Phe-Val-Arg-p-nitroanilide substrate by four freeze/thaw cycles
high-speed counter-current chromatography coupled with the reverse micelle solvent system consisting of 0.10 g/ml cetyltrimethylammonium bromide (CTAB)/isooctane-hexylalcohol and 0.05 M sodium phosphate buffer is successfully applied to separate bromelain from pineapple fruit
using centrifugation, salt precipitation technique, dialysis, ion-exchange chromatography. Ion exchange chromatography using diethylaminoethyl cellulose (DEAE) anion exchangers maintain the structural integrity of purified bromelain and thereby the product exhibits better proteolytic activity than crude extract
use as additive to prevent lymphedema by reducing lymphocongestion, detritus, viscosity of the exsudate and stimulation of phagocytosis of associated leukocytes, use as additive for radiotherapy and surgery due to wound healing effect and reductive effect on inflammation and edema, use as additives for chemotherapy due to inhibitory effects on malignant growth of tumor cells
PHYSICOCHEMICAL PROPERTIES OF THE PROTEOLYTIC ENZYME FROM THE LATEX OF THE MILKWEED, ASCLEPIAS SPECIOSA TORR. SOME COMPARISONS WITH OTHER PROTEASES : III. KINETICS OF THE HEAT INACTIVATION OF PAPAIN, BROMELIN, AND ASCLEPAIN.