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Literature summary for 3.4.22.33 extracted from
- Bromelain enzymes (1976), Methods Enzymol., 45, 475-485.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mercurials | cysteine restores activity; mercaptoethanol restores activity | Ananas comosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4 | - |
Nalpha-benzoyl-Arg amide | - |
Ananas comosus |  |
| 43 | - |
N-benzoyl-L-Arg ethyl ester | - |
Ananas comosus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ananas comosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ananas comosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Ananas comosus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| angiotensin II + H2O | cleavage between Tyr4-Ile5 | Ananas comosus | hydrolyzed angiotensin II | cleavage between Tyr4-Ile5 | ? | |
| bradykinin + H2O | cleavage between Gly4-Phe5 and Phe5-Ser6 | Ananas comosus | fragments of bradykinin | cleavage between Gly4-Phe5 and Phe5-Ser6 | ? | |
| additional information | enzyme catalyzes synthesis of acylamino acid anilides | Ananas comosus | ? | - |
? | |
| N-Benzoyl-L-Arg ethyl ester + H2O | - |
Ananas comosus | N-Benzoyl-L-Arg + ethanol | - |
? | |
| Nalpha-Benzoyl-Arg amide + H2O | - |
Ananas comosus | Nalpha-Benzoyl-Arg + NH3 | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
casein | Ananas comosus |
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Release 2023.1 (January 2023)
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