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Literature summary for 3.4.22.33 extracted from

  • Cabral, H.; Leopoldino, A.M.; Tajara, E.H.; Greene, L.J.; Faca, V.M.; Mateus, R.P.; Ceron, C.R.; de Souza Judice, W.A.; Julianod, L.; Bonilla-Rodriguez, G.O.
    Preliminary functional characterization, cloning and primary sequence of Fastuosain, a cysteine peptidase isolated from fruits of Bromelia fastuosa (2006), Protein Pept. Lett., 13, 83-89.
    View publication on PubMed
Search for more literature for 3.4.22.33

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol highest activity at 96 mM, at 37°C and pH 7.0 Bromelia fastuosa
dithiothreitol highest activity at 2.5 mM, at pH 7.0 and 37°C Bromelia fastuosa
EDTA 28% increase of activity at 2 mM Bromelia fastuosa
Urea activity decreases in the presence of 4 M urea and reaches the activity of the control again at 8 M when using casein as a substrate Bromelia fastuosa

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Bromelia fastuosa

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 12% residual activity at 0.16 mM Bromelia fastuosa
cystatin
-
 Bromelia fastuosa
Hg2+ 14% residual activity at 0.16 mM Bromelia fastuosa
iodoacetic acid 5% residual activity at 2 mM Bromelia fastuosa
additional information not inhibited by pepstatin Bromelia fastuosa
p-chloromercuribenzoate 15% residual activity at 2 mM Bromelia fastuosa
Phenylmethylsulfonylfluoride 84% residual activity at 2 mM Bromelia fastuosa
Urea at 2.4 M urea the activity decreases continuously to 50% when using N-alpha-benzoyl-DL-arginine-2-naphthylamide as a substrate Bromelia fastuosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 SDS-PAGE Bromelia fastuosa

Organism

Organism UniProt Comment Textmining
Bromelia fastuosa Q6Q2T4 fragment
-

Purification (Commentary)

Purification (Comment) Organism
CM-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephadex G-50 gel filtration Bromelia fastuosa

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Bromelia fastuosa
-
leaf
-
 Bromelia fastuosa
-
stem
-
 Bromelia fastuosa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxyxarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Bromelia fastuosa ?
-
 ?
casein + H2O
-
 Bromelia fastuosa ?
-
 ?
epsilon-NH2-Cap-Ala-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
-
 Bromelia fastuosa ?
-
 ?
epsilon-NH2-Cap-Cys(S-Bzl)-Leu-7-amido-4-methylcoumarin + H2O
-
 Bromelia fastuosa ?
-
 ?
epsilon-NH2-Cap-Leu-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
-
 Bromelia fastuosa ?
-
 ?
epsilon-NH2-Cap-Leu-Gly-7-amido-4-methylcoumarin + H2O
-
 Bromelia fastuosa ?
-
 ?
epsilon-NH2-Cap-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Bromelia fastuosa ?
-
 ?
epsilon-NH2-Cap-Pro-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
-
 Bromelia fastuosa ?
-
 ?
N-alpha-benzoyl-DL-arginine-2-naphthylamide + H2O
-
 Bromelia fastuosa N-alpha-benzoyl-DL-arginine + 2-naphthylamine
-
 ?

Synonyms

Synonyms Comment Organism
fastuosain
-
 Bromelia fastuosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 using casein as a subatrate Bromelia fastuosa
65
-
 using N-alpha-benzoyl-DL-arginine-2-naphthylamide as a substrate Bromelia fastuosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bromelia fastuosa

pH Range

pH Minimum pH Maximum Comment Organism
4 9
-
 Bromelia fastuosa