Information on EC 3.4.11.14 - cytosol alanyl aminopeptidase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.4.11.14
-
RECOMMENDED NAME
GeneOntology No.
cytosol alanyl aminopeptidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
release of an N-terminal amino acid, preferentially alanine, from a wide range of peptides, amides and arylamides
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
exopeptidase, release of N-terminal amino acid
-
CAS REGISTRY NUMBER
COMMENTARY hide
243859-94-1
-
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ala-2-naphthylamide + H2O
Ala + 2-naphthylamine
show the reaction diagram
Ala-4-methyl-coumaryl-7-amide + H2O
Ala + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Ala-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
Ala-7-amido-4-methylcoumarin
Ala + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Ala-Ala + H2O
Ala + Ala
show the reaction diagram
-
45.4% of the activity with Ala-Trp
-
-
?
Ala-Asp + H2O
Ala + Asp
show the reaction diagram
-
11.4% of the activity with Ala-Trp
-
-
?
Ala-Glu + H2O
Ala + Glu
show the reaction diagram
-
19.0% of the activity with Ala-Trp
-
-
?
Ala-Gly + H2O
Ala + Gly
show the reaction diagram
-
17.8% of the activity with Ala-Trp
-
-
?
Ala-Leu + H2O
Ala + Leu
show the reaction diagram
-
27.1% of the activity with Ala-Trp
-
-
?
Ala-p-nitroanilide + H2O
Ala + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Ala-p-nitroanilide + H2O
L-alanine + p-nitroaniline
show the reaction diagram
Ala-Phe + H2O
Ala + Phe
show the reaction diagram
-
42.8% of the activity with Ala-Trp
-
-
?
Ala-Ser + H2O
Ala + Ser
show the reaction diagram
-
22.5% of the activity with Ala-Trp
-
-
?
Ala-Trp + H2O
Ala + Trp
show the reaction diagram
-
-
-
-
?
Ala-Val + H2O
Ala + Val
show the reaction diagram
-
47.3% of the activity with Ala-Trp
-
-
?
Aplysia insulin D + H2O
?
show the reaction diagram
-
Aplysia insulin D degrades slowly under the effects of mAAP
-
-
?
Arg-2-naphthylamide + H2O
Arg + 2-naphthylamide
show the reaction diagram
-
-
-
-
?
Arg-2-naphthylamide + H2O
Arg + 2-naphthylamine
show the reaction diagram
Arg-7-amido-4-methylcoumarin
Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
36% of the activity with Ala-7-amido-4-methylcoumarin
-
-
?
Arg-Arg-7-amido-4-methylcoumarin
?
show the reaction diagram
-
13% of the activity with Ala-7-amido-4-methylcoumarin
-
-
?
CKKQ20KK + H2O
?
show the reaction diagram
-
-
-
-
?
Gly-2-naphthylamide + H2O
Gly + 2-naphthylamine
show the reaction diagram
-
13.7% of the activity with Ala-2-naphthylamide
-
-
?
glycine-4-methyl-coumaryl-7-amide + H2O
glycine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Ile-2-naphthylamide + H2O
Ile + 2-naphthylamide
show the reaction diagram
-
-
-
-
?
KKE-(5-[(2-aminoethyl) amino]-naphthalene-1-sulfonic acid)-Q9K-((4'-dimethylaminoazobenzene-4'-sulfonyl))-K + H2O
?
show the reaction diagram
-
-
-
-
?
KKQ30KK + H2O
?
show the reaction diagram
-
-
-
-
?
L-Ala-2-naphthylamide + H2O
L-Ala + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
L-Arg-enkephalin + H2O
L-Arg + enkephalin
show the reaction diagram
-
-
-
-
?
L-arginine-4-methylcoumaryl-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
L-lysine-4-methylcoumaryl-7-amide + H2O
L-lysine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
L-methionine-4-methylcoumaryl-7-amide + H2O
L-methionine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
L-proline-4-methylcoumaryl-7-amide + H2O
L-proline + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
L-tyrosine-4-methylcoumaryl-7-amide + H2O
L-tyrosine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
L-valine-4-methylcoumaryl-7-amide + H2O
L-valine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Leu-(beta-naphthylamine) + H2O
Leu + naphthylamine
show the reaction diagram
-
-
-
-
?
Leu-2-naphthylamide + H2O
Leu + 2-naphthylamine
show the reaction diagram
Leu-4-methyl-coumaryl-7-amide + H2O
Leu + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Leu-7-amido-4-methylcoumarin
Leu + 7-amino-4-methylcoumarin
show the reaction diagram
-
90% of the activity with Ala-7-amido-4-methylcoumarin
-
-
?
Leu-Ala + H2O
Leu + Ala
show the reaction diagram
-
25.6% of the activity with Ala-Trp
-
-
?
Leu-Gly + H2O
Leu + Gly
show the reaction diagram
-
5.5% of the activity with Ala-Trp
-
-
?
Leu-Leu + H2O
Leu + Leu
show the reaction diagram
Leu-p-nitroanilide + H2O
Leu + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Lys-2-naphthylamide + H2O
Lys + 2-naphthylamine
show the reaction diagram
Lys-7-amido-4-methylcoumarin
Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
93% of the activity with Ala-7-amido-4-methylcoumarin
-
-
?
Lys-Ala-7-amido-4-methylcoumarin
?
show the reaction diagram
-
79% of the activity with Ala-7-amido-4-methylcoumarin
-
-
?
Lys-p-nitroanilide + H2O
Lys + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Met-2-naphthylamide + H2O
Met + 2-naphthylamine
show the reaction diagram
Met-7-amido-4-methylcoumarin
Met + 7-amino-4-methylcoumarin
show the reaction diagram
-
97% of the activity with Ala-7-amido-4-methylcoumarin
-
-
?
Met-enkephalin + H2O
?
show the reaction diagram
-
-
releases only the N-terminal tyrosine
-
?
Met-Lys-bradykinin + H2O
Met + Lys + bradykinin
show the reaction diagram
-
-
-
?
Met-p-nitroanilide + H2O
Met + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Phe-2-naphthylamide + H2O
Phe + 2-naphthylamine
show the reaction diagram
Phe-7-amido-4-methylcoumarin
Phe + 7-amino-4-methylcoumarin
show the reaction diagram
-
84% of the activity with Ala-7-amido-4-methylcoumarin
-
-
?
Phe-Gly + H2O
Phe + Gly
show the reaction diagram
-
19.2% of the activity with Ala-Trp
-
-
?
poly-Glu peptide + H2O
?
show the reaction diagram
-
capable of rapidly digesting poly-Q sequences
-
-
?
Pro-7-amido-4-methylcoumarin
Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
39% of the activity with Ala-7-amido-4-methylcoumarin
-
-
?
Pro-p-nitroanilide + H2O
Pro + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Pro-Phe-Arg-7-amido-4-methylcoumarin
?
show the reaction diagram
-
20% of the activity with Ala-7-amido-4-methylcoumarin
-
-
?
Ser-2-naphthylamide + H2O
Ser + 2-naphthylamine
show the reaction diagram
-
6.0% of the activity with Ala-2-naphthylamide
-
-
?
tau protein + H2O
?
show the reaction diagram
-
-
-
-
?
Thr-2-naphthylamide + H2O
Thr + 2-naphthylamine
show the reaction diagram
-
5.7% of the activity with Ala-2-naphthylamide
-
-
?
Trp-2-naphthylamide + H2O
Trp + 2-naphthylamine
show the reaction diagram
-
19.2% of the activity with Ala-2-naphthylamide
-
-
?
Tyr-7-amido-4-methylcoumarin + H2O
Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
-
99% of the activity with Ala-7-amido-4-methylcoumarin
-
-
?
Val-2-naphthylamide + H2O
Val + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
Val-p-nitroanilide + H2O
Val + p-nitroaniline
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
9.8% increase of activity at 5 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
11-aminoundecanoic acid
-
-
2,2'-bipyridine
-
5.0 mM, 29% inhibition
2,9-Dimethyl-1,10-phenanthroline
-
2.0 mM, 52% inhibition
6-aminohexanoic acid
-
-
6-aminopenicillanic acid
-
-
8-hydroxyquinoline
-
2.0 mM, 84% inhibition
acetonitrile
-
20% complete loss of activity
actinonin
-
0.1 mM, complete inactivation
Ala-Ala
-
inhibits hyrolysis of Ala-2-naphthylamide
Ala-Ala-Ala
-
inhibits hyrolysis of Ala-2-naphthylamide
Ala-Ala-Ala-Ala
-
inhibits hyrolysis of Ala-2-naphthylamide
Ala-Ala-Phe-chloromethyl ketone
-
-
amastatin
anti-PSA immunglobulinb G
-
53.4% residual activity at 0.1 mg/ml
-
antipain
-
0.1 mM, 39% inhibition
Aprotinin
-
82% residual activity at 0.1 mM
Arphamenine A
-
0.1 mM, 80% inactivation
azide
-
92% inhibition at 10 mM
benzamidine
-
1 mM, 14% inhibition
benzylpenicillenic acid
-
-
benzylpenicilloic acid
-
-
bestatin
Cd2+
-
1 mM, complete inhibition
CdCl2
-
6.2% residual activity at 1 mM
chymostatin
-
0.1 mM, 83% inhibition
Cloxacillin
-
-
Co2+
-
1 mM, complete inhibition
Cu2+
-
1 mM, complete inhibition
CuSO4
-
0.6% residual activity at 1 mM
cyanide
-
84% inhibition at 10 mM
D-Leu-Tyr
-
-
Dimethyl formamide
-
-
dodecanedioic acid
-
-
dodecanoic acid
-
-
E-64
-
27.2% residual activity at 0.015 mM
ethanol
-
inactivation above 30%
Gly-Gly-Ala
-
inhibits hyrolysis of Ala-4-nitroanilide
Gly-Gly-Phe
-
inhibits hyrolysis of Ala-4-nitroanilide
hexanedioic acid
-
-
hexanoic acid
-
-
iodoacetamide
-
1 mM, 57% inhibition
iodoacetic acid
-
1 mM, 11% inhibition
KCN
-
10 mM, 84% inhibition
L-arginine
-
19.3% residual activity at 5 mM
L-cysteine
-
non-competitive, 7.3% residual activity at 5 mM
L-isoleucine
-
20.1% residual activity at 5 mM
L-leucine
-
6.9% residual activity at 5 mM
L-phenylalanine
-
21.1% residual activity at 5 mM
L-tryptophan
-
15.5% residual activity at 5 mM
L-valine
-
23.7% residual activity at 5 mM
Leu
-
noncompetitive with Ala-2-naphthylamide, mixed type inhibition with Leu-Leu-Leu, competitive inhibition with Ala-Ala-Ala
Leu-Leu
-
-
Leu-Leu-Leu
leuhistin
-
0.1 mM, complete inactivation
leupeptin
Met
-
noncompetitive with Ala-2-naphthylamide
Met-NH2
-
competitive with Ala-2-naphthylamide
methanol
-
inactivation only above 30%
Methicillin
-
-
Methyl cellosolve
-
40% loss of activity at 40% solvent
Mg2+
-
1 mM, 20% inhibition
Mn2+
-
1 mM, 69% inhibition
Na2S
-
10 mM, 92% inhibition
NaCl
-
hydrolysis of Ala-4-nitropanilide, competitive inhibition at pH 6.6, inhibition is noncompetitive at pH 5.8, inhibition is mixed type at pH 6.2
NEM
-
1 mM, 43% inhibition
Ni2+
-
1 mM, 90% inhibition
Octanoic acid
-
competitive
octylamine
-
competitive
Oxacillin
-
-
oxalate
-
50 mM ammonium oxalate, 71% inhibition
p-dioxane
-
; inactivation only above 30%
PAQ-22
-
-
penicillin
-
-
penicillin G
-
-
Penicillin V
-
-
phebestin
-
-
phosphoramidon
-
0.1 mM, 17% inhibition
PMSF
-
1 mM, 40% inhibition; 1 mM, 64% inhibition
puromycin
puromycin aminonucleoside
-
-
Sulfide
-
-
Tyr
-
noncompetitive with Ala-2-naphthylamide, competitive with Ala-Ala-Ala
ZnSO4
-
24% residual activity at 1 mM
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
5 mM, 2fold stimulation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.123
Ala-2-naphthylamide
-
-
0.235
Ala-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.27
Ala-p-nitroanilide
-
with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C
0.054
Arg-2-naphthylamide
-
-
0.03
Arg-Arg-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.24
glycine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.048
Ile-naphthylamide
-
-
0.035
L-alanine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.0013
L-arginine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.0019
L-leucine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.0052
L-lysine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.01
L-methionine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.0066
L-proline-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.045
L-tyrosine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.0033
L-valine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.071
Leu-2-naphthylamide
-
-
0.22
Leu-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.25
Leu-p-nitroanilide
-
with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C
0.04
Lys-2-naphthylamide
-
-
0.115
Lys-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.11
Lys-Ala-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
2.2
Lys-p-nitroanilide
-
with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C
0.014
Met-2-naphthylamide
-
-
0.115
Met-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.00095
Met-enkephalin
-
-
0.8
Met-p-nitroanilide
-
with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C
0.142
Phe-2-naphthylamide
-
-
0.26
Phe-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.5
Pro-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.47
Pro-p-nitroanilide
-
with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C
0.02
Pro-Phe-Arg-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.17
Tyr-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.042
Val-2-naphthylamide
-
-
0.21
Val-p-nitroanilide
-
with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
141
Ala-2-naphthylamide
Homo sapiens
-
-
13.1
Ala-7-amido-4-methylcoumarin
Rattus norvegicus
-
pH 7.5, 37°C
3.8 - 20.83
Ala-p-nitroanilide
22.2
Arg-2-naphthylamide
Homo sapiens
-
-
0.13
Arg-7-amido-4-methylcoumarin
Rattus norvegicus
-
pH 7.5, 37°C
0.11
Arg-Arg-7-amido-4-methylcoumarin
Rattus norvegicus
-
pH 7.5, 37°C
10.3
glycine-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
6.33
Ile-2-naphthylamide
Homo sapiens
-
-
48.9
L-alanine-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
3.8
L-arginine-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
8.2
L-leucine-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
17.1
L-lysine-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
25.6
L-methionine-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
1.1
L-proline-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
38.5
L-tyrosine-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
0.9
L-valine-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
49.5
Leu-2-naphthylamide
Homo sapiens
-
-
11.3
Leu-7-amido-4-methylcoumarin
Rattus norvegicus
-
pH 7.5, 37°C
3.5 - 19.83
Leu-p-nitroanilide
11.3
Lys-2-naphthylamide
Homo sapiens
-
-
19.5
Lys-7-amido-4-methylcoumarin
Rattus norvegicus
-
pH 7.5, 37°C
4.5
Lys-Ala-7-amido-4-methylcoumarin
Rattus norvegicus
-
pH 7.5, 37°C
89
Lys-p-nitroanilide
Homo sapiens
-
with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C
95.5
Met-2-naphthylamide
Homo sapiens
-
-
14.3
Met-7-amido-4-methylcoumarin
Rattus norvegicus
-
pH 7.5, 37°C
30
Met-p-nitroanilide
Homo sapiens
-
with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C
74.5
Phe-2-naphthylamide
Homo sapiens
-
-
12.8
Phe-7-amido-4-methylcoumarin
Rattus norvegicus
-
pH 7.5, 37°C
1.5
Pro-7-amido-4-methylcoumarin
Rattus norvegicus
-
pH 7.5, 37°C
16.9 - 23.17
Pro-p-nitroanilide
0.97
Pro-Phe-Arg-7-amido-4-methylcoumarin
Rattus norvegicus
-
pH 7.5, 37°C
7.7
Tyr-7-amido-4-methylcoumarin
Rattus norvegicus
-
pH 7.5, 37°C
3.83
Val-2-naphthylamide
Homo sapiens
-
-
1.43
Val-p-nitroanilide
Homo sapiens
-
with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
40
glycine-4-methyl-coumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
167985
1400
L-alanine-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
6948
3000
L-arginine-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
8715
4200
L-leucine-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
5021
3300
L-lysine-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
14231
2500
L-methionine-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
18538
170
L-proline-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
10280
870
L-tyrosine-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
15225
260
L-valine-4-methylcoumaryl-7-amide
Danio rerio
-
pH 7.0, 37°C
19571
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.028
1,10-phenanthroline
-
-
17
6-aminopenicillanic acid
-
-
1.9
Ala-Ala
-
hydrolysis of Ala-2-naphthylamide
0.32
Ala-Ala-Ala
-
hydrolysis of Ala-4-nitroanilide
0.089
Ala-Ala-Ala-Ala
-
hydrolysis of Ala-2-naphthylamide
0.00004
amastatin
-
-
0.007
benzylpenicillenic acid
-
-
44
benzylpenicilloic acid
-
-
0.51
Cloxacillin
-
-
0.012
EGTA
-
-
7
Gly-Gly-Ala
-
hydrolysis of Ala-2-naphthylamide
1.4
Gly-Gly-Phe
-
hydrolysis of Ala-4-nitroanilide
0.27
L-cysteine
-
pH 7.0, 37°C
0.23
Leu-Leu
-
hydrolysis of Ala-2-naphthylamide
0.054
Leu-Leu-Leu
-
hydrolysis of Ala-2-naphthylamide
2.4
Methicillin
-
-
1.6
Oxacillin
-
-
9.8
penicillin G
-
-
13
Penicillin V
-
-
0.076
Sulfide
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00007
bestatin
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.00008
PAQ-22
Homo sapiens
-
non-competitive, pH not specified in the publication, temperature not specified in the publication
0.0006
puromycin
0.0025
puromycin aminonucleoside
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45.7
-
hydrolysis of L-alanyl-2-naphthylamide
70
-
hydrolysis of L-alanyl-2-naphthylamide
76.7
-
-
281.7
-
1 mM Ala-p-nitroanilide in 0.05 M Tris/HCl, pH 7.5
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 8.5
-
pH 5.5: about 55% of maximal activity, pH 8.5: about 50% of maximal activity, hydrolysis of Ala-7-amido-4-methylcoumarin
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.6 - 4.7
-
chromatofocusing
5.25
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
lowest activity
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
20% of the PSA is associated with the membrane
Manually annotated by BRENDA team
additional information
-
enzyme activity is found in all fractions studied
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90000
-
1 * 90000, SDS-PAGE in presence of 2-mercaptoethanol
98000
-
gel filtration
100000
-
gel filtration
110000
-
SDS-PAGE
119500
-
2 * 119500, SDS-PAGE
120000
-
2 * 120000, SDS-PAGE
223000
-
sedimentation equilibrium centrifugation
236000
-
sedimentation equilibrium centrifugation
242000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 9.5
-
4°C, 24 h, stable
647029
7
-
activity decreases rapidly when pH value higher or lower than 7.0
717515
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 45
-
unstable above 50°C
40
-
30 min, stable up to
50
-
30 min, 50% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
inactivated after only one cycle of freezing and thawing
-
when the enzyme is treated at 60°C with EDTA the inhibition is no longer reversible and there is a concomitant loss of zinc
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, pH 7.5, 20 mM Tris-buffered saline, stable for several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
glutathione Sepharose column chromatography and Superdex-200 gel filtration
-
glutathione-Sepharose column chromatography and Superdex-200 gel filtration
-
ion exchange chromatography
-
ion exchange chromatography (DEAE), hydroxyapatite chromatography, ammonium sulfate precipitation, hydrophobic interaction chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL-21 (DE3) cells
-
expressed in Mus musculus
-
expressed in Mus musculus brain
-
expression in COS-7 cells
-
GFP fusion protein expressed in muscles of adult CD1 male mice and Drosophila
-
overexpressed in SH-SY5Y cell
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
after cell treatment with CoCl2
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Q598X
-
mutation truncates the protein downstream of the active site
W538X
-
mutation truncates the protein downstream of the active site
additional information
-
mutant mpa1 lacking enzymic activity exhibits reduced fertility, and produces shorter siliques bearing a lower number of seeds. In the mutant line, both male and female meiosis are defective leading to abnormal chromosome segregation
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
coexpression of Drosophila puromycin-sensitive aminopeptidase and beta-amyloid in the flies' brains improves their lifespan, protects against locomotor deficits, and reduces brain beta-amyloid levels by clearing the beta-amyloid plaque-like deposits. Puromycin-sensitive aminopeptidase localizes to the cytoplasm. Therefore, puromycin-sensitive aminopeptidase and beta-amyloid are unlikely to be in the same cellular compartment. Beta-amyloid is not a proteolytic substrate for puromycin-sensitive aminopeptidase in vitro. The enzymatic activity of puromycin-sensitive aminopeptidase is not required for rescuing beta-amyloid toxicity in neuronal SH-SY5Y cells