Information on EC 3.2.2.7 - adenosine nucleosidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.2.2.7
-
RECOMMENDED NAME
GeneOntology No.
adenosine nucleosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
adenosine + H2O = D-ribose + adenine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of N-glycosyl bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
adenine and adenosine salvage II
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-
Purine metabolism
-
-
purine metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
adenosine ribohydrolase
Also acts on adenosine N-oxide.
CAS REGISTRY NUMBER
COMMENTARY hide
9075-41-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
mangrove shrub, high activity of enzyme
-
-
Manually annotated by BRENDA team
var. gemmifera
-
-
Manually annotated by BRENDA team
mangrove shrub
-
-
Manually annotated by BRENDA team
L. cv. Catimor
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
artichoke
-
-
Manually annotated by BRENDA team
mangrove shrub
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
mangrove shrub
-
-
Manually annotated by BRENDA team
alfalfa
-
-
Manually annotated by BRENDA team
no activity in Ceratodon purpureus
-
-
-
Manually annotated by BRENDA team
no activity in Equisetum arvense
-
-
-
Manually annotated by BRENDA team
no activity in Leishmania braziliensis
-
-
-
Manually annotated by BRENDA team
no activity in Leishmania donovani
-
-
-
Manually annotated by BRENDA team
no activity in Leishmania mexicana
-
-
-
Manually annotated by BRENDA team
no activity in Leishmania tarentolae
-
-
-
Manually annotated by BRENDA team
no activity in Populus alba
-
-
-
Manually annotated by BRENDA team
mangrove shrub
-
-
Manually annotated by BRENDA team
mangrove shrub
-
-
Manually annotated by BRENDA team
three forms: R1, R2 and Lf
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-
Manually annotated by BRENDA team
mangrove shrub
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Ulmus carpinifolia
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-deoxyadenosine + H2O
6-aminopurine + 2-deoxy-D-ribofuranose
show the reaction diagram
3'-deoxyadenosine + H2O
6-aminopurine + 3-deoxy-D-ribofuranose
show the reaction diagram
-
6-aminopurine-9-3'-deoxy-beta-D-ribofuranoside, cordycepin, very low activity
-
?
5'-deoxyadenosine + H2O
6-aminopurine + 5-deoxy-D-ribofuranose
show the reaction diagram
-
6-aminopurine-9-5'-deoxy-beta-D-ribofuranoside, best substrate
-
?
6-aminopurine-9-beta-D-ribofuranosyl-N1-oxide + H2O
6-aminopurine-N1-oxide + D-ribofuranose
show the reaction diagram
6-benzylaminopurine riboside + H2O
6-benzylaminopurine + D-ribose
show the reaction diagram
-
low activity
-
?
7-methyl-6-aminopurine-9-beta-D-ribofuranoside + H2O
7-methyl-6-aminopurine + D-ribofuranose
show the reaction diagram
-
5fold faster hydrolysis compared to adenosine
-
?
adenosine + H2O
6-aminopurine + D-ribofuranose
show the reaction diagram
adenosine + H2O
D-ribose + adenine
show the reaction diagram
guanosine + H2O
2-amino-1,9-dihydropurine-6-on + D-ribofuranose
show the reaction diagram
-
2-amino-1,9-dihydro-6-oxopurine-9-beta-D-ribofuranoside, two third lowered activity compared to adenosine as substrate
-
?
inosine + H2O
hypoxanthine + D-ribose
show the reaction diagram
-
9-ribosylhydroxanthine, low activity
-
?
N6-(DELTA2-isopentenyl)-adenosine + H2O
N6-(DELTA2-isopentenyl)-6-aminopurine + D-ribofuranose
show the reaction diagram
-
N6-(DELTA2-isopentenyl)-6-aminopurine-9-beta-D-ribofuranoside
-
?
purine riboside + H2O
purine + D-ribose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2'-deoxyadenosine + H2O
6-aminopurine + 2-deoxy-D-ribofuranose
show the reaction diagram
adenosine + H2O
6-aminopurine + D-ribofuranose
show the reaction diagram
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2'-deoxyadenosine
3'-deoxyadenosine
-
Ki: four times weaker than for 2'-deoxyadenosine
6-Methylaminopurine
adenine
adenosine
-
78% inhibition at 0.5 mM
Cu2+
-
marked inhibition at 1 mM
cytokinin
-
plant hormones, inhibition less strong than for adenine
dithiothreitol
-
60% inhibition if enzyme is preincubated with 10 mM
HgCl2
-
strong inhibition at 1 mM
Inosine
-
at 0.2 mM: 8.5% inhibition for form Lf, 10.5% inhibition for form R2, form R1 not inhibited
N6-Benzyladenosine
N6-furfuryladenosine
-
53% inhibition at 0.5 mM
N6-isopentenyladenosine
-
inhibition between 28.6% and 36.7% for forms R1, R2 and Lf at 0.2 mM
p-chloromercuribenzoate
-
20% inhibition at 1 mM
trans-6-(4-hydroxy-3-methyl-2-butenylamino)-9-beta-D-ribofuranosylpurine
-
70% inhibition at 0.5 mM
zeatin riboside
-
competitive inhibition between 32% and 25% for forms R1, R2 and Lf at 0.2 mM
Zn2+
-
marked inhibition at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12
2'-deoxyadenosine
-
in MES buffer, pH 5.4
0.001 - 0.7
adenosine
0.002
N6-(DELTA2-isopentenyl)-adenosine
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-
additional information
additional information
-
kinetics
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06
adenosine
Lupinus luteus
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-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
2'-deoxyadenosine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.003
-
first activity after 2 days of seedling growth
0.049
-
maximum activity after 9 days of seedling growth
7
-
for enzyme I
7.97
-
for enzyme III
12.84
-
for enzyme II
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 4.5
-
optimum activity varies with buffer used
4.5
-
for enzyme II
5.4
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in 50 mM MES/KOH buffer
6 - 7
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in phosphate buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
for enzyme I
50
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for enzymes II and III
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 60
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-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
adenosine nucleosidase activity is negligible in freshly prepared slices, activity is dramatically enhanced in wounded slices
Manually annotated by BRENDA team
-
of roots and in root tips
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
-
alpha2, 2 * 33000, non-reducing SDS-PAGE
34600
-
2 * 34600, SDS-PAGE
59000
-
gel filtration
62400
-
sucrose density gradient centrifugation, gel filtration
66000
-
gel filtration
120000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
stable within, enzymes I, II and III
137109
9
-
50% activity reatained
137051
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
-
stable for 15 days
40
-
rapid loss of activity, enzymes I, II and III
60
-
activity sharply decreases above
115
-
40% remaining activity after 15 min for Sephadex G-50 stabilized enzyme
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
highly stable at 4C and under deep-freeze conditions, no reducing agent required for stability
-
labile during course of purification, addition of dithiothreitol required, 50% loss of activity if stored at -20C in one week
-
presence of salt essential
-
stable against repeated freezing and thawing cycles
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Methanol
-
remains active up to 20%, v/v
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10C, 10 mM NaHCO3, 4 months
-
-20C, 95% retaining activity after 30 days
-
-20C, one month, gradually loss of activity on storage at 4C
-
4C, 20 mM phosphate, pH 7.2, 10 mM 2-mercaptoethanol, 70% activity remains, 12 weeks
-
long-term storage at room temperature for Sephadex G-50 stabilized enzyme, 100% activity remains after 4.5 years
-
storage in dried state in Sephadex G-25 beads remains stability for several months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
form R2 to homogeneity, forms R1 and Lf partial
-
near homogeneity
-
partial
partial, three forms isolated: enzymes I, II and III
-
to homogeneity, chromatography steps
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
URH1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, functional complementation of a yeast mutant, expression in transgenic Arabidopsis thaliana mutant plants under control of the CaMV 35S promoter
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
mutants expressing the Arabidopsis thaliana enzyme or the homologue from Oryza sativa exhibit resistance toward toxic fluorouridine, fluorouracil, and fluoroorotic acid. Mutants with increased and decreased nucleosidase activity are delayed in germination