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Literature summary for 3.2.2.7 extracted from

  • Mazelis, M.; Creveling, R.K.
    An adenosine hydrolase from Brussels sprouts (1963), J. Biol. Chem., 238, 3358-3361.
    View publication on PubMed
Search for more literature for 3.2.2.7

General Stability

General Stability Organism
stable against repeated freezing and thawing cycles Brassica oleracea

Inhibitors

Inhibitors Comment Organism Structure
adenine 38% inhibition at 9 mM Brassica oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 adenosine
-
 Brassica oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2'-deoxyadenosine + H2O Brassica oleracea hydrolysis at the same rate as for adenosine 6-aminopurine + 2-deoxy-D-ribofuranose
-
 ?
2'-deoxyadenosine + H2O Brassica oleracea 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside 6-aminopurine + 2-deoxy-D-ribofuranose
-
 ?
adenosine + H2O Brassica oleracea 6-aminopurine-9-beta-D-ribofuranoside 6-aminopurine + D-ribofuranose
-
 ir

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
 var. gemmifera
-

Purification (Commentary)

Purification (Comment) Organism
partial Brassica oleracea

Source Tissue

Source Tissue Comment Organism Textmining
sprout
-
 Brassica oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.62
-
-
 Brassica oleracea

Storage Stability

Storage Stability Organism
-10°C, 10 mM NaHCO3, 4 months Brassica oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosine + H2O hydrolysis at the same rate as for adenosine Brassica oleracea 6-aminopurine + 2-deoxy-D-ribofuranose
-
 ?
2'-deoxyadenosine + H2O 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside Brassica oleracea 6-aminopurine + 2-deoxy-D-ribofuranose
-
 ?
adenosine + H2O 6-aminopurine-9-beta-D-ribofuranoside Brassica oleracea 6-aminopurine + D-ribofuranose
-
 ir

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10
-
 stable for 15 days Brassica oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 4.5 optimum activity varies with buffer used Brassica oleracea