General Stability | Organism |
---|---|
stable against repeated freezing and thawing cycles | Lupinus luteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
adenosine | - |
Lupinus luteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
gel filtration | Lupinus luteus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyadenosine + H2O | Lupinus luteus | one third lowered activity compared to adenosine as substrate | 6-aminopurine + 2-deoxy-D-ribofuranose | - |
? | |
2'-deoxyadenosine + H2O | Lupinus luteus | 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside | 6-aminopurine + 2-deoxy-D-ribofuranose | - |
? | |
adenosine + H2O | Lupinus luteus | component of the purine salvage pathway | 6-aminopurine + D-ribofuranose | - |
? | |
adenosine + H2O | Lupinus luteus | 6-aminopurine-9-beta-D-ribofuranoside | 6-aminopurine + D-ribofuranose | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Methanol | remains active up to 20%, v/v | Lupinus luteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lupinus luteus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Lupinus luteus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Lupinus luteus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.6 | - |
- |
Lupinus luteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyadenosine + H2O | one third lowered activity compared to adenosine as substrate | Lupinus luteus | 6-aminopurine + 2-deoxy-D-ribofuranose | - |
? | |
2'-deoxyadenosine + H2O | 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside | Lupinus luteus | 6-aminopurine + 2-deoxy-D-ribofuranose | - |
? | |
3'-deoxyadenosine + H2O | 6-aminopurine-9-3'-deoxy-beta-D-ribofuranoside, cordycepin, very low activity | Lupinus luteus | 6-aminopurine + 3-deoxy-D-ribofuranose | - |
? | |
5'-deoxyadenosine + H2O | 6-aminopurine-9-5'-deoxy-beta-D-ribofuranoside, best substrate | Lupinus luteus | 6-aminopurine + 5-deoxy-D-ribofuranose | - |
? | |
6-benzylaminopurine riboside + H2O | low activity | Lupinus luteus | 6-benzylaminopurine + D-ribose | - |
? | |
adenosine + H2O | component of the purine salvage pathway | Lupinus luteus | 6-aminopurine + D-ribofuranose | - |
? | |
adenosine + H2O | 6-aminopurine-9-beta-D-ribofuranoside | Lupinus luteus | 6-aminopurine + D-ribofuranose | - |
? | |
guanosine + H2O | 2-amino-1,9-dihydro-6-oxopurine-9-beta-D-ribofuranoside, two third lowered activity compared to adenosine as substrate | Lupinus luteus | 2-amino-1,9-dihydropurine-6-on + D-ribofuranose | - |
? | |
inosine + H2O | 9-ribosylhydroxanthine, low activity | Lupinus luteus | hypoxanthine + D-ribose | - |
? | |
purine riboside + H2O | low activity | Lupinus luteus | purine + D-ribose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33000-36000, SDS-PAGE | Lupinus luteus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
adenosine | - |
Lupinus luteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Lupinus luteus |