General Stability | Organism |
---|---|
labile during course of purification, addition of dithiothreitol required, 50% loss of activity if stored at -20°C in one week | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | marked inhibition at 1 mM | Hordeum vulgare | |
HgCl2 | strong inhibition at 1 mM | Hordeum vulgare | |
p-chloromercuribenzoate | 20% inhibition at 1 mM | Hordeum vulgare | |
Zn2+ | marked inhibition at 1 mM | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
adenosine | - |
Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
gel filtration | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyadenosine + H2O | Hordeum vulgare | hydrolyzes at 96% of the rate of adenosine | 6-aminopurine + 2-deoxy-D-ribofuranose | - |
? | |
2'-deoxyadenosine + H2O | Hordeum vulgare | 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside | 6-aminopurine + 2-deoxy-D-ribofuranose | - |
? | |
adenosine + H2O | Hordeum vulgare | 6-aminopurine-9-beta-D-ribofuranoside | 6-aminopurine + D-ribofuranose | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
barley | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | malt | Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.2 | - |
- |
Hordeum vulgare |
Storage Stability | Organism |
---|---|
4°C, 20 mM phosphate, pH 7.2, 10 mM 2-mercaptoethanol, 70% activity remains, 12 weeks | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyadenosine + H2O | hydrolyzes at 96% of the rate of adenosine | Hordeum vulgare | 6-aminopurine + 2-deoxy-D-ribofuranose | - |
? | |
2'-deoxyadenosine + H2O | 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside | Hordeum vulgare | 6-aminopurine + 2-deoxy-D-ribofuranose | - |
? | |
adenosine + H2O | 6-aminopurine-9-beta-D-ribofuranoside | Hordeum vulgare | 6-aminopurine + D-ribofuranose | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Hordeum vulgare |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | - |
Hordeum vulgare |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
activity sharply decreases above | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
same activity in Tris or phosphate buffer, no requirement for inorganic phosphate | Hordeum vulgare |