Information on EC 3.2.1.65 - levanase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.2.1.65
-
RECOMMENDED NAME
GeneOntology No.
levanase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Random hydrolysis of (2->6)-beta-D-fructofuranosidic linkages in (2->6)-beta-D-fructans (levans) containing more than 3 fructose units
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
hydrolysis of O-glycosyl bond
additional information
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Starch and sucrose metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
(2->6)-beta-D-fructan fructanohydrolase
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2,6-beta-D fructan fructohydrolase
-
-
2,6-beta-D-fructan fructanohydrolase
-
-
-
-
6-FEHs
-
-
endolevanase
Bacillus licheniformis IBt1
-
-
endolevanase
-
-
endolevanase
Treponema zioleckii kT
-
-
-
exo-levanase
Gluconacetobacter diazotrophicus SRT4
-
-
exolevanase
Gluconacetobacter diazotrophicus SRT4
-
-
-
fructan-6-exohydrolase
-
-
fructose-releasing exo-levanase
-
-
LevB1
Bacillus licheniformis IBt1
-
-
LsdB
Gluconacetobacter diazotrophicus SRT4
-
;
-
LsdB
Gluconacetobacter diazotrophicus SRT4
-
-
fructose-releasing exo-levanase
Gluconacetobacter diazotrophicus SRT4
-
-
-
LeA
additional information
-
-
levan hydrolase
-
-
-
-
levanase
additional information
-
-
CAS REGISTRY NUMBER
COMMENTARY
9041-11-6
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Bacillus licheniformis ATCC 14580
gene sacC
UniProt
Manually annotated by BRENDA team
Bacillus licheniformis IBt1
-
UniProt
Manually annotated by BRENDA team
var. Laevanivorans
-
-
Manually annotated by BRENDA team
strain SRT4, gene lsdB
-
-
Manually annotated by BRENDA team
Gluconacetobacter diazotrophicus SRT4
strain SRT4
UniProt
Manually annotated by BRENDA team
Gluconacetobacter diazotrophicus SRT4
strain SRT4
-
-
Manually annotated by BRENDA team
Gluconacetobacter diazotrophicus SRT4
strain SRT4, gene lsdB
-
-
Manually annotated by BRENDA team
no activity in Escherichia coli
-
-
-
Manually annotated by BRENDA team
Pseudomonas sp. No. 43
-
-
-
Manually annotated by BRENDA team
Pseudomonas strain K-52
-
-
-
Manually annotated by BRENDA team
Rhodotorula sp.
-
-
-
Manually annotated by BRENDA team
Streptomyces sp. 366L
-
-
-
Manually annotated by BRENDA team
Treponema zioleckii kT
-
-
-
Manually annotated by BRENDA team
isolated from a Tunisian thermal source
-
-
Manually annotated by BRENDA team
yeasts
Sacch. cerevisiae
-
-
Manually annotated by BRENDA team
yeasts
Saccharomyces anamensis , Sacch. fermentati, Sacch. florenzani, Sacch. fragilis, Sacch. rosei, Schizosaccharomyces pombe, Schwanniomyces alluvius, Schw. castellii, Torulopsis colliculosa; Sacch. cerevisiae
-
-
Manually annotated by BRENDA team
additional information
enzymatic activity from the sediment of the Gilau dam reservoir, Cluj county
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
-
the enzyme belongs to the glycosyl hydrolase family 32, GH32
evolution
Bacillus licheniformis ATCC 14580, Bacillus licheniformis IBt1
-
the enzyme belongs to the glycosyl hydrolase family 32, GH32
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-kestose + H2O
D-fructose
show the reaction diagram
Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus SRT4
-
-
?
3 levan + 7 H2O
10 levanbiose
show the reaction diagram
Pseudomonas sp. No. 43
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Pseudomonas strain K-52
-
-
-
-
?
D(+)-raffinose + H2O
beta-D-fructose + alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranoside
show the reaction diagram
Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus SRT4
-
-
-
?
D(+)-raffinose + H2O
D-fructose
show the reaction diagram
-
-
?
D-(+)-xylose + H2O
?
show the reaction diagram
Pseudomonas strain K-52
-
-
-
-
?
dextran + H2O
?
show the reaction diagram
Pseudomonas strain K-52
-
-
-
-
?
fructose + H2O
?
show the reaction diagram
Pseudomonas strain K-52
-
-
-
-
?
galactose + H2O
?
show the reaction diagram
Pseudomonas strain K-52
-
-
-
-
?
glucose + H2O
?
show the reaction diagram
Pseudomonas strain K-52
-
-
-
-
?
inulin + H2O
?
show the reaction diagram
-
-
-
-
?
inulin + H2O
?
show the reaction diagram
Rhodotorula sp.
-
-
-
-
-
inulin + H2O
?
show the reaction diagram
-
-
-
-
-
inulin + H2O
?
show the reaction diagram
Bacillus licheniformis, Bacillus licheniformis ATCC 14580
-
-
-
?
inulin + H2O
D-fructose
show the reaction diagram
Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus SRT4
without formation of intermediate oligofructans
-
?
inulin + H2O
D-fructose + ?
show the reaction diagram
Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus SRT4
-
without formation of intermediate oligofructans
-
?
lactose + H2O
?
show the reaction diagram
Pseudomonas strain K-52
-
-
-
-
?
levan + H2O
?
show the reaction diagram
-
-
-
-
?
levan + H2O
?
show the reaction diagram
-
-
-
?
levan + H2O
?
show the reaction diagram
-
substrate from Erwinia herbicola
-
-
?
levan + H2O
?
show the reaction diagram
Bacillus licheniformis IBt1
-
-
-
?
levan + H2O
?
show the reaction diagram
Gluconacetobacter diazotrophicus SRT4
-
substrate from Erwinia herbicola
-
-
?
levan + H2O
oligofructosides
show the reaction diagram
-
-
-
?
levan + H2O
oligofructosides
show the reaction diagram
-
-
-
?
levan + H2O
oligofructosides
show the reaction diagram
-
-
-
?
levan + H2O
oligofructosides
show the reaction diagram
-
-
-
?
levan + H2O
oligofructosides
show the reaction diagram
-
-
-
?
levan + H2O
oligofructosides
show the reaction diagram
-
-
-
-
levan + H2O
oligofructosides
show the reaction diagram
-
-
-
-
levan + H2O
oligofructosides
show the reaction diagram
yeasts
-
-
-
-
levan + H2O
oligofructosides
show the reaction diagram
yeasts
-
-
-
-
levan + H2O
levanheptaose
show the reaction diagram
Streptomyces sp. 366L
-
-
-
?
levan + H2O
fructose
show the reaction diagram
Rhodotorula sp.
-
-
-
?
levan + H2O
D-fructose
show the reaction diagram
-
-
-
-
levan + H2O
D-fructose
show the reaction diagram
4fold higher rate of hydrolysis compared to inulin, without formation of intermediate oligofructans, no activity with D(+)-melezitose
-
?
levan + H2O
D-fructose
show the reaction diagram
Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus SRT4
-
without formation of intermediate oligofructans, no activity with D(+)-melezitose
-
?
levan + H2O
D-fructose
show the reaction diagram
Gluconacetobacter diazotrophicus SRT4
4fold higher rate of hydrolysis compared to inulin, without formation of intermediate oligofructans, no activity with D(+)-melezitose
-
?
levan + H2O
fructose + beta-1,2-oligofructans
show the reaction diagram
Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus SRT4
-
-
?
levan + H2O
levanbiose + D-fructose
show the reaction diagram
-
-
final products, thinlayer chromatography analysis
?
levan + H2O
O-beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranoside + ?
show the reaction diagram
Bacillus licheniformis, Bacillus licheniformis IBt1
almost no differences in specific activity at 1% w/v levan are found when LevB1 is assayed with levans obtained from the levansucrases of Bacillus subtilis or Leuconostoc mesenteroides ATCC 8293, but it is 10fold less active toward levan from Leuconostoc mesenteroides NRRL-B512 levansucrase
major product
?
levan-type fructans (beta-2,6)
D-fructose
show the reaction diagram
-
-
-
?
levans + H2O
oligofructosides
show the reaction diagram
-
-
-
?
levans + H2O
oligofructosides
show the reaction diagram
-
-
-
?
levans + H2O
oligofructosides
show the reaction diagram
-
-
-
?
levans + H2O
oligofructosides
show the reaction diagram
-
-
-
?
levans + H2O
oligofructosides
show the reaction diagram
-
-
-
?
levans + H2O
oligofructosides
show the reaction diagram
-
-
-
?
raffinose + H2O
?
show the reaction diagram
Pseudomonas strain K-52
-
-
-
-
?
soluble starch + H2O
?
show the reaction diagram
Pseudomonas strain K-52
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
-
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
Rhodotorula sp.
-
-
-
-
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
-
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus SRT4
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram
Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus SRT4
-
-
-
?
Timothy grass fructan + H2O
?
show the reaction diagram
Treponema zioleckii, Treponema zioleckii kT
-
-
-
-
?
xylan + H2O
?
show the reaction diagram
Pseudomonas strain K-52
-
-
-
-
?
levan + H2O
fructose
show the reaction diagram
-
-
-
?
levan + H2O
D-fructose
show the reaction diagram
additional information
-
-
-
?
maltose + H2O
?
show the reaction diagram
Pseudomonas strain K-52
-
-
-
-
?
additional information
?
-
a levanase with high inulinase activity. The strain ATCC 14580 efficiently produces 2,3-butanediol from fructose at 50C
-
-
-
additional information
?
-
-
the exo-acting enzyme is capable of hydrolyzing not only beta-2,6-fructosyl linkages, but also beta-2,1-fructosyl linkages
-
-
-
additional information
?
-
Bacillus licheniformis ATCC 14580
a levanase with high inulinase activity. The strain ATCC 14580 efficiently produces 2,3-butanediol from fructose at 50C
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
inulin + H2O
?
show the reaction diagram
Bacillus licheniformis, Bacillus licheniformis ATCC 14580
Q65GW7
-
-
-
?
levan + H2O
?
show the reaction diagram
-
-
-
-
?
levan + H2O
?
show the reaction diagram
W8GV60
-
-
-
?
levan + H2O
fructose + beta-1,2-oligofructans
show the reaction diagram
Q43998
-
-
?
levan + H2O
?
show the reaction diagram
Bacillus licheniformis IBt1
W8GV60
-
-
-
?
levan + H2O
fructose + beta-1,2-oligofructans
show the reaction diagram
Gluconacetobacter diazotrophicus SRT4
Q43998
-
-
?
levans + H2O
oligofructosides
show the reaction diagram
-
-
-
?
levans + H2O
oligofructosides
show the reaction diagram
-
-
-
?
levans + H2O
oligofructosides
show the reaction diagram
-
-
-
?
levans + H2O
oligofructosides
show the reaction diagram
-
-
-
?
levans + H2O
oligofructosides
show the reaction diagram
-
-
-
?
levans + H2O
oligofructosides
show the reaction diagram
-
-
-
?
additional information
?
-
Bacillus licheniformis, Bacillus licheniformis ATCC 14580
Q65GW7
a levanase with high inulinase activity. The strain ATCC 14580 efficiently produces 2,3-butanediol from fructose at 50C
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
NaCl
-
slight activation at 75 mM
Mn2+
-
slight activation at 75 mM
additional information
not significantly influenced by 1 mM Ca2+, Mg2+, or Fe3+, at 30C, pH 6.0, 1% levan, for 6 h
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
Pseudomonas sp. No. 43
-
-
Ag+
Pseudomonas strain K-52
-
-
Ag+
Streptomyces sp. 366L
-
-
Ag+
Pseudomonas sp. No. 43
-
-
Al3+
Pseudomonas sp. No. 43
-
-
Ammonium oxalate
Rhodotorula sp.
-
-
Ammonium oxalate
Pseudomonas sp. No. 43
-
-
ammonium persulfate
Rhodotorula sp.
-
-
ascorbic acid
Rhodotorula sp.
-
-
ascorbic acid
Pseudomonas sp. No. 43
-
-
Ba2+
Pseudomonas sp. No. 43
-
-
Ca2+
Rhodotorula sp.
-
-
Ca2+
Pseudomonas sp. No. 43
-
-
Co2+
Rhodotorula sp.
-
-
Co2+
Streptomyces sp. 366L
-
-
Co2+
Pseudomonas sp. No. 43
-
-
Cu2+
Pseudomonas strain K-52
-
-
Cu2+
Rhodotorula sp.
-
-
CuSO4
1 mM, pH 6.0, 1% levan, for 6 h at 30C, 27% inhibition
diethyl dicarbonate
Pseudomonas strain K-52
-
-
EDTA
Pseudomonas strain K-52
-
-
EDTA
Rhodotorula sp.
-
-
EDTA
Pseudomonas sp. No. 43
-
-
EDTA
-
slight inhibition at 75 mM
Fe2+
Pseudomonas strain K-52
-
-
Fe2+
-
slight inhibition at 75 mM
glutathione
Rhodotorula sp.
-
-
Hg2+
Pseudomonas strain K-52
-
-
Hg2+
Rhodotorula sp.
-
-
Hydrazine sulfate
Rhodotorula sp.
-
-
iodoacetate
Streptomyces sp. 366L
-
-
KMnO4
Rhodotorula sp.
-
-
KMnO4
Pseudomonas sp. No. 43
-
-
Mg2+
Rhodotorula sp.
-
-
Mg2+
Pseudomonas sp. No. 43
-
-
Mg2+
-
slight inhibition at 75 mM
Mn2+
Streptomyces sp. 366L
-
-
Mn2+
Pseudomonas sp. No. 43
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
Streptomyces sp. 366L
-
-
SDS
Streptomyces sp. 366L
-
-
SDS
1%, pH 6.0, 1% levan, for 6 h at 30C, 97% inhibition
Sodium citrate
Rhodotorula sp.
-
-
Sodium thioglycollate
Rhodotorula sp.
-
-
Sodium thioglycollate
Pseudomonas sp. No. 43
-
-
Sucrose
-
weak inhibitory effect at high concentrations
Tris buffer
-
-
Zn2+
Streptomyces sp. 366L
-
-
Zn2+
-
slight inhibition at 75 mM
additional information
not inhibited by EDTA, dithiothreitol and beta-mercaptoethanol
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
Rhodotorula sp.
-
-
2-mercaptoethanol
-
slight activation at 75 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
6.8
inulin
-
-
0.0012
levan
-
-
0.5
levan
-
-
65
Sucrose
-
-
10
tetraose
-
-
-
0.97
levan
Rhodotorula sp.
-
-
additional information
additional information
Michaelis-Menten kinetics
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.09
-
U/ml, inulin
0.25
-
intracellular wild-type enzyme
0.3
-
BG4103-strain
0.33
-
extracellular mutant AD6 enzyme
0.4
-
QB167-strain
0.5
-
U/ml, inulin
0.52
-
extracellular mutant AD8 enzyme
0.69
-
intracellular mutant AD9 enzyme
0.71
-
extracellular mutant AD9 enzyme
0.73
-
intracellular mutant AD8 enzyme
0.75
-
intracellular mutant AD6 enzyme
1.8
purified recombinant enzyme, pH 6.0, 35C
14.24
-
extracellular wild-type enzyme
30.5
Pseudomonas sp. No. 43
-
U/mg
34.4
Rhodotorula sp.
-
-
37.9
crude recombinant enzyme, pH 6.5, 50C, substrate inulin
39.2
Pseudomonas strain K-52
-
-
58
-
levan, pH 5.0, at 30C
128
-
U/mg
226.7
-
purified native enzyme, pH 6.5, 40C
987
purified recombinant enzyme, pH 6.5, 50C, substrate inulin
4309
Streptomyces sp. 366L
-
U/mg
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4
-
not active below
5
-
levan, at 30C; sucrose, at 30C
5.5
-
no activity loss at pH 6.0 and 60C for 20 min
6
Rhodotorula sp.
-
-
6.5
-
no activity below pH 5.5
6.5
recombinant enzyme
7
Pseudomonas strain K-52
-
-
7
Rhodotorula sp.
-
-
7
Streptomyces sp. 366L
-
rapidly decreased activity below and above pH 7.0
7
Pseudomonas sp. No. 43
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 7
inactive below pH 4.0, decreased activity above pH 7.0
5 - 7
-
levan, at 30C, activity decreases at pH 4.0 and at pH values above 7.0; sucrose, at 30C, activity decreases at pH 4.0 and at pH values above 7.0
5.5 - 6.5
-
in presence of levan extended to pH 4.6-7.5
5.5 - 6.5
Rhodotorula sp.
-
-
5.5 - 7
higher activity, recombinant enzyme
6 - 10
Streptomyces sp. 366L
-
20C
6 - 9
Pseudomonas strain K-52
-
4C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35
Pseudomonas strain K-52
-
-
40
Rhodotorula sp.
-
-
40
Streptomyces sp. 366L
-
pH 7.0; rapidly inactivated above 45C
40
Pseudomonas sp. No. 43
-
-
40 - 50
-
sucrose, pH 5.0
45
-
not active above 55C
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20 - 60
-
activity range
30 - 40
Pseudomonas strain K-52
-
-
40 - 50
-
not active above 55C
45 - 55
-
-
50 - 70
activity range, profile overview
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Gluconacetobacter diazotrophicus SRT4
-
-
-
Manually annotated by BRENDA team
Gluconacetobacter diazotrophicus SRT4
-
-
-
Manually annotated by BRENDA team
additional information
-
enzyme expression analysis under different growth conditions, overview
Manually annotated by BRENDA team
additional information
-
maximum production of levanase activity is achieved after 36 h
Manually annotated by BRENDA team
additional information
Gluconacetobacter diazotrophicus SRT4
-
enzyme expression analysis under different growth conditions, overview
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Treponema zioleckii kT
-
-
-
Manually annotated by BRENDA team
Pseudomonas sp. No. 43
-
-
-
Manually annotated by BRENDA team
Gluconacetobacter diazotrophicus SRT4
-
-
-
-
Manually annotated by BRENDA team
-
small extent transported into periplasmic space
Manually annotated by BRENDA team
Treponema zioleckii kT
-
-
-
-
Manually annotated by BRENDA team
Gluconacetobacter diazotrophicus SRT4
-
-
-
-
Manually annotated by BRENDA team
Treponema zioleckii kT
-
outer membrane
-
Manually annotated by BRENDA team
additional information
-
mature LsdB is unlikely to attach cell membranes
-
Manually annotated by BRENDA team
additional information
Gluconacetobacter diazotrophicus SRT4
-
mature LsdB is unlikely to attach cell membranes
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
36000
Pseudomonas sp. No. 43
-
gel filtration
136770
38000
Pseudomonas strain K-52
-
SDS-PAGE and gel filtration
136767
39000
Rhodotorula sp.
-
SDS-PAGE
136768
57000
-
SDS-PAGE
655383
58400
calculation from sequence of cDNA
655294
73000
-
SDS-PAGE
136759
74000
-
in vitro transcription and translation experiments
136757
75000
-
SDS-PAGE
136756
75000
-
-
136766
75870
-
DNA sequence analysis
136759, 136762
78000
Streptomyces sp. 366L
-
SDS-PAGE
136769
80000
Streptomyces sp. 366L
-
gel filtration
136769
86000
-
SDS-PAGE and DNA sequence analysis
136771
89000
-
SDS-PAGE
136758
100000
-
SDS-PAGE
136762
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 60000, SDS-PAGE
?
x * 66000, recombinnat enzyme, SDS-PAGE
?
x * 56400, SDS-PAGE
?
Bacillus licheniformis ATCC 14580
-
x * 66000, recombinnat enzyme, SDS-PAGE
-
?
Bacillus licheniformis IBt1
-
x * 56400, SDS-PAGE
-
?
Treponema zioleckii kT
-
x * 60000, SDS-PAGE
-
monomer
-
SDS-PAGE
monomer
-
SDS-PAGE
monomer
Streptomyces sp. 366L
-
gel filtration; SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
no modification
Gluconacetobacter diazotrophicus SRT4
-
;
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.6 - 7.5
-
-
136763
5 - 11
purified recombinant enzyme, 50C, 1 h, stable at
731185
5.5 - 6.5
Rhodotorula sp.
-
-
136768
6 - 8
Pseudomonas sp. No. 43
-
20C, stable up to 50C at pH 7.0; activity decreases rapidly below pH 6.0 and above pH 8.0
136770
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
-25
-
stable for several weeks
136760
-25
-
stable for several weeks
136761
-21
-
-
136761
-21
-
rapidly inactivated above
136761
4
Rhodotorula sp.
-
-
136768
30 - 35
purified recombinant enzyme, 24 h, completely stable at
732847
40
Pseudomonas sp. No. 43
-
stable up to 50C but rapidly inactivated above
136770
40
-
crude extracellular enzyme, pH 6.4, stable up to
731934
50
purified recombinant enzyme, pH 6.5, 1 h, stable up to
731185
55
-
rapidly inactivated at 60C
136766
55
purified recombinant enzyme, pH 6.5, 1 h, 89% activity remaining
731185
60
irreversibly inactivated above
655294
60
-
levan, pH 5.0, irreversibly inactived above 60C; sucrose, pH 5.0, irreversibly inactived above 60C
655383
65
purified recombinant enzyme, pH 6.5, 1 h, inactivation
731185
70
-
crude extracellular enzyme, pH 6.4, inactivation
731934
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, phosphate buffer, pH 6.0
-
-15C, several months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
by immobilization-nickel affinity chromatography, centrifugation, column chromatography and dialysis
-
-
Pseudomonas sp. No. 43
-
-
Pseudomonas strain K-52
-
-
Rhodotorula sp.
-
-
Streptomyces sp. 366L
-
ammonium sulfate precipitation and Sephadex A-50 gel filtration
-
native extracellular enzyme 397fold from culture supernatant by ammonium sulfate fractionation, dialysis, gel filtration, and anion exchange chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli
gene sacC, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression in Escherichia coli strain BL21(DE3)
expression in B. subtilis
-
expression in Escherichia coli
-
expression in Lactobacillus plantarum
-
expression in Lactobacillus-Escherichia coli
-
expression in Pichia pastoris
-
expression in Escherichia coli
expression in Pichia pastoris
-
gene lsdB, organized in one operon with gene lsdA, encoding levansucrase, organization of the lsdAB operon and relevant features of the intergenic sequence, expression analysis, overview
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
lsdB transcription is repressed by D-glucose
-
lsdB transcription is induced during growth with low fructose concentrations of 0.44 to 33 mM
-
lsdB transcription is repressed by D-glucose
Gluconacetobacter diazotrophicus SRT4
-
-
lsdB transcription is induced during growth with low fructose concentrations of 0.44 to 33 mM
Gluconacetobacter diazotrophicus SRT4
-
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
type II secretion mutants AD6, AD8, and AD9, unable to secrete LsdA due to nptII insertions in the type II secretion, the mutants fail to transfer LsdB across the outer membrane, impeding levan hydrolysis
additional information
Gluconacetobacter diazotrophicus SRT4
-
type II secretion mutants AD6, AD8, and AD9, unable to secrete LsdA due to nptII insertions in the type II secretion, the mutants fail to transfer LsdB across the outer membrane, impeding levan hydrolysis
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
-
pathogenicity of periodontal disease
synthesis
the enzyme is used for simultaneous saccharification and fermentation of inulin to 2,3-butanediol. A fed-batch simultaneous saccharification and fermentation yields 103.0 g/liter 2,3-butanediol in 30 h, with a high productivity of 3.4 g/liter/h
synthesis
the enzyme is uzilized for production of beta2-6 fructose oligosaccharides (levan-type FOS) through a sequential reactionwith levan produced from sucrose by bacterial levansucrases, method development, overview
synthesis
Bacillus licheniformis ATCC 14580
-
the enzyme is used for simultaneous saccharification and fermentation of inulin to 2,3-butanediol. A fed-batch simultaneous saccharification and fermentation yields 103.0 g/liter 2,3-butanediol in 30 h, with a high productivity of 3.4 g/liter/h
-
synthesis
Bacillus licheniformis IBt1
-
the enzyme is uzilized for production of beta2-6 fructose oligosaccharides (levan-type FOS) through a sequential reactionwith levan produced from sucrose by bacterial levansucrases, method development, overview
-
medicine
-
mitogenic for human B lymphocytes and activates the alternative complement pathway
nutrition
-
production of pure fructose syrups
synthesis
-
synthesis of various products such as ethanol or aceton-butanol
synthesis
-
commercial production of ultra-high-fructose syrups
synthesis
Gluconacetobacter diazotrophicus SRT4
-
commercial production of ultra-high-fructose syrups
-
agriculture
Pseudomonas sp. No. 43
-
plant differentiation, growth promoting activities for some enteric bacteria
medicine
Pseudomonas sp. No. 43
-
enhancing effect on the immune system and other physiological functions
nutrition
Pseudomonas sp. No. 43
-
energy source; production of noncaloric sweeteners
synthesis
Pseudomonas sp. No. 43
-
protection from aging
medicine
Pseudomonas strain K-52
-
enhancing effect on the immune system and other physiological functions
agriculture
Rhodotorula sp.
-
slime dissolving for more effective penetration of biocides inside the cell wall
synthesis
Rhodotorula sp.
-
paper industry
agriculture
Streptomyces sp. 366L
-
plant differentiation, growth promoting activities for some enteric bacteria
medicine
Streptomyces sp. 366L
-
enhancing effect on the immune system and other physiological functions
nutrition
Streptomyces sp. 366L
-
energy source; production of noncaloric sweeteners
synthesis
Streptomyces sp. 366L
-
protection from aging
analysis
additional information
-
the enzymatic activity can be used to characterize the aquatic ecosystems impurity degree, the evolution prognosis and water quality maintenance, the Gilau dam reservoir sediment has a decreased and quite unstable enzymatic potential