Cloned (Comment) | Organism |
---|---|
gene lsdB, organized in one operon with gene lsdA, encoding levansucrase, organization of the lsdAB operon and relevant features of the intergenic sequence, expression analysis, overview | Gluconacetobacter diazotrophicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | type II secretion mutants AD6, AD8, and AD9, unable to secrete LsdA due to nptII insertions in the type II secretion, the mutants fail to transfer LsdB across the outer membrane, impeding levan hydrolysis | Gluconacetobacter diazotrophicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Gluconacetobacter diazotrophicus | - |
- |
additional information | mature LsdB is unlikely to attach cell membranes | Gluconacetobacter diazotrophicus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconacetobacter diazotrophicus | - |
gene lsdB | - |
Gluconacetobacter diazotrophicus SRT4 | - |
gene lsdB | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | enzyme expression analysis under different growth conditions, overview | Gluconacetobacter diazotrophicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.25 | - |
intracellular wild-type enzyme | Gluconacetobacter diazotrophicus |
0.33 | - |
extracellular mutant AD6 enzyme | Gluconacetobacter diazotrophicus |
0.52 | - |
extracellular mutant AD8 enzyme | Gluconacetobacter diazotrophicus |
0.69 | - |
intracellular mutant AD9 enzyme | Gluconacetobacter diazotrophicus |
0.71 | - |
extracellular mutant AD9 enzyme | Gluconacetobacter diazotrophicus |
0.73 | - |
intracellular mutant AD8 enzyme | Gluconacetobacter diazotrophicus |
0.75 | - |
intracellular mutant AD6 enzyme | Gluconacetobacter diazotrophicus |
14.24 | - |
extracellular wild-type enzyme | Gluconacetobacter diazotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
levan + H2O | substrate from Erwinia herbicola | Gluconacetobacter diazotrophicus | ? | - |
? | |
levan + H2O | substrate from Erwinia herbicola | Gluconacetobacter diazotrophicus SRT4 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
exolevanase | - |
Gluconacetobacter diazotrophicus |
LsdB | - |
Gluconacetobacter diazotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Gluconacetobacter diazotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Gluconacetobacter diazotrophicus |
Organism | Comment | Expression |
---|---|---|
Gluconacetobacter diazotrophicus | lsdB transcription is repressed by D-glucose | down |
Gluconacetobacter diazotrophicus | lsdB transcription is induced during growth with low fructose concentrations of 0.44 to 33 mM | up |