Information on EC 2.5.1.48 - cystathionine gamma-synthase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.5.1.48
-
RECOMMENDED NAME
GeneOntology No.
cystathionine gamma-synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
O4-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
-
-
gamma-replacement
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
-
-
Biosynthesis of secondary metabolites
-
-
Cysteine and methionine metabolism
-
-
L-methionine biosynthesis I
-
-
Metabolic pathways
-
-
methionine metabolism
-
-
Selenocompound metabolism
-
-
Sulfur metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
O4-succinyl-L-homoserine:L-cysteine S-(3-amino-3-carboxypropyl)transferase
A pyridoxal-phosphate protein. Also reacts with hydrogen sulfide and methanethiol as replacing agents, producing homocysteine and methionine, respectively. In the absence of thiol, can also catalyse beta,gamma-elimination to form 2-oxobutanoate, succinate and ammonia.
CAS REGISTRY NUMBER
COMMENTARY hide
9030-70-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
enzyme type I, preference for O-succinyl-L-homoserine + L-cysteine as substrates
-
-
Manually annotated by BRENDA team
enzyme type II, preference for O-acetyl-L-homoserine + L-cysteine as substrates
-
-
Manually annotated by BRENDA team
two enzymes: enzyme type I, preference for O-succinyl-L-homoserine + L-cysteine as substrates and enzyme type II, preference for O-acetyl-L-homoserine + L-cysteine as substrates
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
enzyme type II, preference for O-acetyl-L-homoserine + L-cysteine as substrates
-
-
Manually annotated by BRENDA team
enzyme type II, preference for O-acetyl-L-homoserine + L-cysteine as substrates
-
-
Manually annotated by BRENDA team
strain SS
UniProt
Manually annotated by BRENDA team
strain SS
UniProt
Manually annotated by BRENDA team
Neptunia amplexicaulis
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Pseudomonas dacunhae
enzyme type I, preference for O-succinyl-L-homoserine + L-cysteine as substrates
-
-
Manually annotated by BRENDA team
enzyme type I, preference for O-succinyl-L-homoserine + L-cysteine as substrates
-
-
Manually annotated by BRENDA team
spinach
-
-
Manually annotated by BRENDA team
wheat
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-cystathionine + acetate
O-acetyl-L-homoserine + L-cysteine
show the reaction diagram
-
-
-
-
r
L-cystathionine + H2O
L-cysteine + NH3 + 2-oxobutyrate
show the reaction diagram
-
beta-elimination
-
?
L-cystathionine + H2O
L-homocysteine + NH3 + pyruvate
show the reaction diagram
L-cystathionine + L-cysteine
L-cystathionine + L-cysteine
show the reaction diagram
-
-
observed via 35S-exchange
-
O-acetyl-L-homoserine + 2-mercaptoethanol
S-hydroxyethyl-L-homocysteine + acetate
show the reaction diagram
-
150% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + 2-mercaptopropionate
S-methylcarboxymethyl-L-homoserine + acetate
show the reaction diagram
-
50% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + 3-mercaptopropionate
S-carboxyethyl-L-homocysteine + acetate
show the reaction diagram
-
65% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + allyl mercaptan
S-allyl-L-homocysteine + acetate
show the reaction diagram
-
4% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + benzyl mercaptan
S-benzyl-L-homocysteine + acetate
show the reaction diagram
-
41% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + CH3SH
L-methionine + acetate
show the reaction diagram
-
11% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + D-Cys
D-allocystathionine + acetate
show the reaction diagram
-
101% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + D-homocysteine
meso-homolanthionine + acetate
show the reaction diagram
-
125% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + ethyl mercaptan
L-thionine + acetate
show the reaction diagram
-
24% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + isobutyl mercaptan
S-isobutyl-L-homocysteine + acetate
show the reaction diagram
-
13% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + isopropyl mercaptan
S-isopropyl-L-homocysteine + acetate
show the reaction diagram
-
1% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + L-Cys ethyl ester
S-(L-2-amino-2-ethoxycarbonyl)-L-homocysteine + acetate
show the reaction diagram
-
15% relative activity to O-acetyl-L-homoserine + L-cysteine
i.e. L-cystathionine monoethyl ester + acetate
?
O-acetyl-L-homoserine + L-Cys methyl ester
S-(L-2-amino-2-methoxycarbonyl)-L-homocysteine + acetate
show the reaction diagram
O-acetyl-L-homoserine + L-cysteine
L-cystathionine + acetate
show the reaction diagram
O-acetyl-L-homoserine + L-homocysteine
L-homolanthionine + acetate
show the reaction diagram
-
92% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + m-thiocresol
S-m-tolyl-L-homocysteine + acetate
show the reaction diagram
-
150% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + N-acetyl-L-Cys
S-(L-acetylamino-2-carboxyethyl)-L-homocysteine + acetate
show the reaction diagram
-
12% relative activity to O-acetyl-L-homoserine + L-cysteine
i.e. mono-N-acetyl-L-cystathionine + acetate
?
O-acetyl-L-homoserine + n-butyl mercaptan
S-n-butyl-L-homocysteine + acetate
show the reaction diagram
-
5% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + n-propyl mercaptan
S-n-propyl-L-homocysteine + acetate
show the reaction diagram
-
13% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + o-thiocresol
S-o-tolyl-L-homocysteine + acetate
show the reaction diagram
-
3% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + p-nitrothiophenol
S-p-nitrophenyl-L-homocysteine + acetate
show the reaction diagram
-
41% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + p-thiocresol
S-p-tolyl-L-homocysteine + acetate
show the reaction diagram
-
25% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + phenyl mercaptan
S-phenyl-L-homocysteine + acetate
show the reaction diagram
-
98% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + S2-
L-homocysteine + acetate
show the reaction diagram
-
163% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + sec-butyl mercaptan
S-sec-butyl-L-homocysteine + acetate
show the reaction diagram
-
5% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + tert-butyl mercaptan
S-tert-butyl-L-homosysteine + acetate
show the reaction diagram
-
2% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + thioacetic acid
S-acetyl-L-homocysteine + acetate
show the reaction diagram
-
3% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + thioglycolate ethyl ester
S-ethoxycarbonyl-L-homocysteine + acetate
show the reaction diagram
-
348% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + thioglycolate n-butyl ester
S-n-butoxycarbonyl-L-homocysteine + acetate
show the reaction diagram
-
274% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-acetyl-L-homoserine + thiosalicylic acid
S-o-carboxyphenyl-L-homocysteine + acetate
show the reaction diagram
-
63% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-malonyl-L-homoserine + L-cysteine
L-cystathionine + malonate
show the reaction diagram
-
14% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-phenyl-L-homoserine + L-cysteine
L-cystathionine + phenol
show the reaction diagram
-
1% relative activity to O-acetyl-L-homoserine + L-cysteine
-
?
O-phospho-L-homoserine + L-cysteine
L-cystathionine + phosphate
show the reaction diagram
O-phospho-L-homoserine + L-cysteine ethyl ester
S-(L-2-amino-2-ethoxycarbonyl)-L-homocysteine + phosphate
show the reaction diagram
-
1% relative activity to O-phospho-L-homoserine + L-cysteine
-
-
?
O-phospho-L-homoserine + L-cysteine methyl ester
S-(L-2-amino-2-methoxycarbonyl)-L-homocysteine + phosphate
show the reaction diagram
-
15% relative activity to O-phospho-L-homoserine + L-cysteine
-
-
?
O-phospho-L-homoserine + L-selenocysteine
selenocystathionine + phosphate
show the reaction diagram
-
-
-
?
O-phospho-L-homoserine + S2-
L-homocysteine + phosphate
show the reaction diagram
O-succinyl-L-homoserine
succinate + pyruvate + NH3
show the reaction diagram
-
elimination reaction
-
-
?
O-succinyl-L-homoserine + 2-mercaptoethanol
S-ethyl-L-homocysteine + succinate
show the reaction diagram
-
32% of the activity with L-cysteine
-
-
?
O-succinyl-L-homoserine + beta-mercaptopropionate
S-carboxyethyl-L-homocysteine + succinate
show the reaction diagram
-
-
-
?
O-succinyl-L-homoserine + CH3SH
L-methionine + succinate
show the reaction diagram
O-succinyl-L-homoserine + D-cysteine
?
show the reaction diagram
-
20% of the activity with L-cysteine
-
-
?
O-succinyl-L-homoserine + H2O
succinate + 2-oxobutyrate + NH3
show the reaction diagram
O-succinyl-L-homoserine + H2S
L-homocysteine + succinate
show the reaction diagram
O-succinyl-L-homoserine + L-cysteine
cystathionine + succinate
show the reaction diagram
O-succinyl-L-homoserine + L-cysteine
O-acetyl-L-homocysteine + L-cysteine
show the reaction diagram
O-succinyl-L-homoserine + L-selenocysteine
selenocystathionine + succinate
show the reaction diagram
O-succinyl-L-homoserine + sodium sulfide
L-homocysteine + sodium succinate
show the reaction diagram
-
62% of the activity with L-cysteine
-
-
?
O-succinyl-L-serine + H2O
succinate + pyruvate + NH3
show the reaction diagram
O-succinyl-L-serine + L-cysteine
lanthionine + succinate
show the reaction diagram
-
-
-
?
O-succinyl-L-serine + L-homocysteine
succinate + L-cystathionine
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
O-succinyl-L-homoserine + L-cysteine
cystathionine + succinate
show the reaction diagram
O-succinyl-L-homoserine + L-cysteine
O-acetyl-L-homocysteine + L-cysteine
show the reaction diagram
O-succinyl-L-homoserine + L-selenocysteine
selenocystathionine + succinate
show the reaction diagram
-
first step in conversion of selenocysteine to volatile dimethylselenide
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1S,3aS,4R,6aR)-1,4-bis(1,3-benzodioxol-5-yl)hexahydropentalen-3a(1H)-ol
i.e. paulownin, NPLC604, noncompetitive, significant activity in the inhibition of Helicobacter pylori strain SS1 growth with MIC of 15 mg/ml
(1S,3aS,4S,6aS)-1,4-di(3,4,5-trimethoxy)-phenyloctahydropentalene
i.e. yangambin, NPLC605, noncompetitive, significant activity in the inhibition of Helicobacter pylori strain SS1 growth with MIC of 10 mg/ml
(Z)-3-(2-phosphonethen-1-yl)pyridine-2-carboxylic acid
-
Ki: 0.04 mM
-
(Z)-3-(2-phosphonoethen-1-yl)pyridine-2-carboxylic acid
-
-
2,2-dimethyl-3,4-dihydro-2H-benzo[g]chromene-5,10-dione
i.e. alpha-lapachone, NPLC518, noncompetitive, significant activity in the inhibition of Helicobacter pylori strain SS1 growth with MIC of 2.6 mg/ml
3-(phosphonomethyl)pyridine-2-carboxylic acid
-
-
3-Methyl-2-benzothiazolinone hydrazone
-
-
4-(phosphonomethyl)pyridine-2-carboxylic acid
6-hydroxy-2,2-dimethyl-3,4-dihydro-2H-benzo[g]chromene-5,10-dione
i.e. 9-hydroxy-alpha-lapachone, NPLC519, noncompetitive, significant activity in the inhibition of Helicobacter pylori strain SS1 growth with MIC of 2.5 mg/ml
aminooxyacetic acid
-
-
beta-mercaptopropionate
chloromercuriphenylsulfonate
-
inhibition of gamma-elimination
cystathionine
-
competitive, gamma-elimination
D-cycloserine
-
-
D-Cysteine
-
inhibition of gamma-elimination
dithiothreitol
-
10% inhibition of beta-elimination from O-succinyl-L-serine
DL-(E)-2-amino-5-phosphono-3-pentenoic acid
DL-propargylglycine
-
irreversible
hydroxylamine
L-cysteine
L-homocysteine
L-methionine
-
2 mM, decreases the expression level of CGS
N-ethylmaleimide
-
inhibition of gamma-elimination
phenylhydrazine
-
-
propargylglycine
S-adenosyl-L-homocysteine
-
-
S-adenosyl-L-methionine
Semicarbazide
-
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-threonine
-
2 mM, increases the expression level of CGS
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.6
2-mercaptoethanol
-
-
100
CH3SH
-
pH 7.3, 30C, gamma-replacement reaction
3
H2S
-
pH 7.3, 30C, gamma-replacement reaction
0.11 - 2.4
L-cystathionine
0.1 - 4.07
L-cysteine
0.89
L-cysteine methyl ester
-
-
0.07
L-selenocysteine
-
-
64.5
O-acetyl-L-homoserine
1.4 - 7.1
O-phospho-L-homoserine
2.85
O-phospho-L-serine
-
-
0.05 - 90
O-succinyl-L-homoserine
0.7 - 1.2
O-Succinyl-L-serine
0.6
S2-
-
pH 7.5, 30C
0.55
Sodium sulfide
-
-
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.67
cystathionine
Escherichia coli
-
pH 8.2, 25C, 460 mol product formed per mol of subunit
5.6 - 121
L-cystathionine
117 - 121
L-cysteine
0.15 - 130
O-succinyl-L-homoserine
0.133 - 3.83
O-Succinyl-L-serine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2 - 1060
L-cystathionine
0.41 - 470
L-cysteine
0.002 - 90
O-succinyl-L-homoserine
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06
(1S,3aS,4R,6aR)-1,4-bis(1,3-benzodioxol-5-yl)hexahydropentalen-3a(1H)-ol
25C, pH 9.0
0.031
(1S,3aS,4S,6aS)-1,4-di(3,4,5-trimethoxy)-phenyloctahydropentalene
25C, pH 9.0
0.04
(Z)-3-(2-phosphonethen-1-yl)pyridine-2-carboxylic acid
-
pH 7.5, 37C
-
0.45
(Z)-3-(2-phosphonoethen-1-yl)pyridine-2-carboxylic acid
-
-
0.019
2,2-dimethyl-3,4-dihydro-2H-benzo[g]chromene-5,10-dione
25C, pH 9.0
0.2
3-(phosphonomethyl)pyridine-2-carboxylic acid
-
-
0.045 - 0.3
4-(phosphonomethyl)pyridine-2-carboxylic acid
0.016
6-hydroxy-2,2-dimethyl-3,4-dihydro-2H-benzo[g]chromene-5,10-dione
25C, pH 9.0
0.0011 - 0.027
DL-(E)-2-amino-5-phosphono-3-pentenoic acid
0.018
DL-propargylglycine
-
-
9.5
S-adenosyl-L-methionine
-
pH 7.5, 37C
additional information
additional information
-
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.019
(1S,3aS,4R,6aR)-1,4-bis(1,3-benzodioxol-5-yl)hexahydropentalen-3a(1H)-ol
Helicobacter pylori
Q1M0P5
25C, pH 9.0
0.027
(1S,3aS,4S,6aS)-1,4-di(3,4,5-trimethoxy)-phenyloctahydropentalene
Helicobacter pylori
Q1M0P5
25C, pH 9.0
0.011
2,2-dimethyl-3,4-dihydro-2H-benzo[g]chromene-5,10-dione
Helicobacter pylori
Q1M0P5
25C, pH 9.0
0.009
6-hydroxy-2,2-dimethyl-3,4-dihydro-2H-benzo[g]chromene-5,10-dione
Helicobacter pylori
Q1M0P5
25C, pH 9.0
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.403
25C, pH 9.0
3.7
-
plasmid pSL109
10.5
-
O-succinyl-L-homoserine + L-cysteine, gamma-replacement reaction
18.7
-
O-succinyl-L-homoserine + L-cysteine, gamma-replacement reaction
37
-
plasmid pSL123
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
-
replacement reaction
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
O-succinyl-L-homoserine + H2O, 12% of the reaction rate at pH 8.2; O-succinyl-L-serine + H2O, 28% of the reaction rate at pH 8.2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56000
-
SDS-PAGE
160000
165000
-
gel filtration
194000
-
gel filtration
215000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 41655, SDS-PAGE
tetramer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure at 1.5 A resulution
in silico modeling and pyridoxal 5'-phosphate cofactor docking study
-
to 1.9 A resolution. Cofactor pyridoxal 5'-phosphate binds tightly to Lys208 with a covalent-bond length ranging between 1.3 and 1.4 A. The cofactor is stabilized by a series of hydrogen bonds from Gly86, Met87, Asn158, Asp183 and Ser205 from one monomer and Tyr56 and Arg58 from the second monomer
crystals grown by sitting drop vapour diffusion against a reservoir containing 100 mM MES-NaOH
-
to 2.4 A resolution, tetragonal space group I41
-
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
EDTA and dithiothreitol stabilize
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15C, protein concentration 10 mg/ml, 6-12 months stable
-
-20C, 10 mM potassium phosphate buffer, pH 7.5, 50% glycerol
-
-20C, 20 mM MOPS-NaOH, pH 7.5, 1 mM EDTA, 1 mM DTT, 10% v/v glycerol, 5 days, 50% loss of activity
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned from strain JM103, expression in strain DH10B
-
co-expression of Arabidopsis thaliana cystathionine gamma-synthase and bacterial feedback-insensitive aspartate kinase in tobacco
-
construction of transgenic Brassica juncea plants overexpressing the enzyme
-
expressed in Medicago sativa
-
expressed in Nicotiana tabacum
-
expression in Escherichia coli
expression of the CYS3 gene in Escherichia coli, the protein shows activities of EC 4.4.1.1, EC 4.4.1.8, and EC 2.5.1.8
-
gene CGS1, primer extension studies, wheat germ in vitro translation in presence of S-adenosyl-L-methionine results in a 5'-truncated enzyme
-
overexpression in Escherichia coli
-
overexpression of full-length enzyme and its truncated version that lacks the N-terminal region in transgenic Nicotiana tabacum plants. Transgenic plants expressing both types of enzyme have a significant higher level of Met and S-methyl-Met content in their proteins. Plants expressing full-length enzyme show the same phenotype and developmental pattern as wild-type plants, those expressing the truncated length enzyme show a severely abnormal phenotype. The N-terminal region plays a role in protecting plants from a high level of Met catabolic products such as ethylene
-
the truncated cDNA without putative leader peptide, when cloned into a bacterial expression vector, complements the Escherichia coli metB1 mutant strain LE392
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transgenic plants overexpressing the enzyme under the control of the cauliflower mosaic virus 35S promoter show increased soluble Met and its metabolite S-methyl-Met
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D45A
-
decrease in kcat and increase in Km value of succinyl-L-homoserine substrate
D45F
-
reduction in catalytic effciency, without change in reaction specificity
D45F/E325Y
-
reduction in catalytic effciency, without change in reaction specificity
D45N
-
decrease in kcat and increase in Km value of succinyl-L-homoserine substrate
E325A
-
decrease in kcat and increase in Km values of substrates
E325Q
-
kinetic values similar to wild-type
E325Y
-
reduction in catalytic effciency, without change in reaction specificity
N227A
-
decrease in kcat value of succinyl-L-homoserine substrate
R106A
-
the Arg residues at positions 48, 106 and 361 tether the distal and alpha-carboxylate moieties, respectively, of the succinyl-L-homoserine substrate
R106K
-
the Arg residues at positions 48, 106 and 361 tether the distal and alpha-carboxylate moieties, respectively, of the succinyl-L-homoserine substrate
R361A
-
almost complete loss of activity
R361K
-
the Arg residues at positions 48, 106 and 361 tether the distal and alpha-carboxylate moieties, respectively, of the succinyl-L-homoserine substrate
R48A
-
almost complete loss of activity
R48K
-
the Arg residues at positions 48, 106 and 361 tether the distal and alpha-carboxylate moieties, respectively, of the succinyl-L-homoserine substrate
R49A
-
decrease in kcat value
R49K
-
3fold increase in Km value for both succinyl-L-homoserine and L-cysteine
S326A
-
decrease in kcat and increase in Km value of succinyl-L-homoserine substrate
Y101F
-
decrease in kcat value
Y46F
-
large decrease in kcat and increase in Km value of succinyl-L-homoserine substrate
additional information
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
unfolding by guanidinium/HCl inactivates the enzyme due to loss of ketoenamine tautomer. Though pyridoxal 5'-phosphate induces difference in secondary structure content, it is unable to provide stabilizing effect during the overall secondary structure unfolding process. It induces tertiary structure stability of the protein thereby counteracting the deleterious effect of denaturant
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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