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Literature summary for 2.5.1.48 extracted from

  • Farsi, A.; Lodha, P.H.; Skanes, J.E.; Los, H.; Kalidindi, N.; Aitken, S.M.
    Interconversion of a pair of active-site residues in Escherichia coli cystathionine gamma-synthase, E. coli cystathionine beta-lyase, and Saccharomyces cerevisiae cystathionine gamma-lyase and development of tools for the investigation of their mechanisms (2009), Biochem. Cell Biol., 87, 445-457.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D45F reduction in catalytic effciency, without change in reaction specificity Escherichia coli
D45F/E325Y reduction in catalytic effciency, without change in reaction specificity Escherichia coli
E325Y reduction in catalytic effciency, without change in reaction specificity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
L-cystathionine wild-type, 25°C, pH 7.8 Escherichia coli
0.26
-
L-cystathionine mutant E325Y 25°C, pH 7.8 Escherichia coli
1.3
-
O-succinyl-L-homoserine wild-type, gamma-elimination, 25°C, pH 7.8 Escherichia coli
1.5
-
O-succinyl-L-homoserine mutant D45F/E325Y, gamma-elimination, 25°C, pH 7.8 Escherichia coli
1.7
-
O-succinyl-L-homoserine mutant E325Y, gamma-elimination, 25°C, pH 7.8 Escherichia coli
2
-
O-succinyl-L-homoserine mutant D45F, gamma-elimination, 25°C, pH 7.8 Escherichia coli
2.1
-
O-succinyl-L-homoserine mutant E325Y, gamma-replacement, 25°C, pH 7.8 Escherichia coli
2.4
-
L-cystathionine mutant D45F, 25°C, pH 7.8 Escherichia coli
2.5
-
O-succinyl-L-homoserine wild-type, gamma-replacement, 25°C, pH 7.8 Escherichia coli
5.4
-
O-succinyl-L-homoserine mutant D45F, gamma-replacement, 25°C, pH 7.8 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cystathionine + acetate
-
Escherichia coli O-acetyl-L-homoserine + L-cysteine
-
r
O-acetyl-L-homoserine + L-cysteine gamma-replacement Escherichia coli L-cystathionine + acetate
-
r
O-succinyl-L-homoserine + H2O gamma-elimination Escherichia coli succinate + 2-oxobutyrate + NH3
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.23
-
O-succinyl-L-homoserine mutant D45F/E325Y, gamma-elimination, 25°C, pH 7.8 Escherichia coli
1.39
-
O-succinyl-L-homoserine mutant E325Y, gamma-elimination, 25°C, pH 7.8 Escherichia coli
1.4
-
O-succinyl-L-homoserine mutant D45F, gamma-elimination, 25°C, pH 7.8 Escherichia coli
1.8
-
O-succinyl-L-homoserine wild-type, gamma-elimination, 25°C, pH 7.8 Escherichia coli
5.6
-
L-cystathionine mutant D45F, 25°C, pH 7.8 Escherichia coli
11.1
-
L-cystathionine mutant E325Y 25°C, pH 7.8 Escherichia coli
121
-
L-cystathionine wild-type, 25°C, pH 7.8 Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.69
-
O-succinyl-L-homoserine mutant D45F, gamma-elimination, 25°C, pH 7.8 Escherichia coli
0.8
-
O-succinyl-L-homoserine mutant D45F/E325Y, gamma-elimination, 25°C, pH 7.8 Escherichia coli
0.8
-
O-succinyl-L-homoserine mutant E325Y, gamma-elimination, 25°C, pH 7.8 Escherichia coli
1.35
-
O-succinyl-L-homoserine wild-type, gamma-elimination, 25°C, pH 7.8 Escherichia coli
2
-
L-cystathionine mutant D45F, 25°C, pH 7.8 Escherichia coli
3
-
O-succinyl-L-homoserine mutant D45F, gamma-replacement, 25°C, pH 7.8 Escherichia coli
7
-
O-succinyl-L-homoserine mutant E325Y, gamma-replacement, 25°C, pH 7.8 Escherichia coli
41
-
L-cystathionine mutant E325Y 25°C, pH 7.8 Escherichia coli
49
-
O-succinyl-L-homoserine wild-type, gamma-replacement, 25°C, pH 7.8 Escherichia coli
1060
-
L-cystathionine wild-type, 25°C, pH 7.8 Escherichia coli