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Literature summary for 2.5.1.48 extracted from

  • Jaworski, A.F.; Lodha, P.H.; Manders, A.L.; Aitken, S.M.
    Exploration of the active site of Escherichia coli cystathionine gamma-synthase (2012), Protein Sci., 21, 1662-1671.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.48

Protein Variants

Protein Variants Comment Organism
D45A decrease in kcat and increase in Km value of succinyl-L-homoserine substrate Escherichia coli
D45N decrease in kcat and increase in Km value of succinyl-L-homoserine substrate Escherichia coli
E325A decrease in kcat and increase in Km values of substrates Escherichia coli
E325Q kinetic values similar to wild-type Escherichia coli
N227A decrease in kcat value of succinyl-L-homoserine substrate Escherichia coli
R106A the Arg residues at positions 48, 106 and 361 tether the distal and alpha-carboxylate moieties, respectively, of the succinyl-L-homoserine substrate Escherichia coli
R106K the Arg residues at positions 48, 106 and 361 tether the distal and alpha-carboxylate moieties, respectively, of the succinyl-L-homoserine substrate Escherichia coli
R361A almost complete loss of activity Escherichia coli
R361K the Arg residues at positions 48, 106 and 361 tether the distal and alpha-carboxylate moieties, respectively, of the succinyl-L-homoserine substrate Escherichia coli
R48A almost complete loss of activity Escherichia coli
R48K the Arg residues at positions 48, 106 and 361 tether the distal and alpha-carboxylate moieties, respectively, of the succinyl-L-homoserine substrate Escherichia coli
R49A decrease in kcat value Escherichia coli
R49K 3fold increase in Km value for both succinyl-L-homoserine and L-cysteine Escherichia coli
S326A decrease in kcat and increase in Km value of succinyl-L-homoserine substrate Escherichia coli
Y101F decrease in kcat value Escherichia coli
Y46F large decrease in kcat and increase in Km value of succinyl-L-homoserine substrate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 L-cysteine mutant R49A, pH 7.8, 25°C Escherichia coli
0.15
-
 L-cysteine mutant Y101F, pH 7.8, 25°C Escherichia coli
0.17
-
 L-cysteine mutant E325A, pH 7.8, 25°C Escherichia coli
0.2
-
 L-cysteine mutant S326A, pH 7.8, 25°C Escherichia coli
0.22
-
 L-cysteine mutant R361K, pH 7.8, 25°C Escherichia coli
0.22
-
 L-cysteine mutant R48K, pH 7.8, 25°C Escherichia coli
0.24
-
 L-cysteine wild-type, pH 7.8, 25°C Escherichia coli
0.29
-
 L-cysteine mutant E325Q, pH 7.8, 25°C Escherichia coli
0.3
-
 L-cysteine mutant N227A, pH 7.8, 25°C Escherichia coli
0.36
-
 L-cysteine mutant R49K, pH 7.8, 25°C Escherichia coli
0.6
-
 L-cysteine mutant Y46F, pH 7.8, 25°C Escherichia coli
0.65
-
 L-cysteine mutant R106K, pH 7.8, 25°C Escherichia coli
0.7
-
 O-succinyl-L-homoserine mutant E325A, pH 7.8, 25°C Escherichia coli
0.8
-
 L-cysteine mutant D45A, pH 7.8, 25°C Escherichia coli
0.9
-
 L-cysteine mutant D45N, pH 7.8, 25°C Escherichia coli
1.1
-
 O-succinyl-L-homoserine mutant E325Q, pH 7.8, 25°C Escherichia coli
1.8
-
 O-succinyl-L-homoserine mutant R48K, pH 7.8, 25°C Escherichia coli
1.9
-
 O-succinyl-L-homoserine mutant Y101F, pH 7.8, 25°C Escherichia coli
3
-
 L-cysteine mutant R106A, pH 7.8, 25°C Escherichia coli
4.4
-
 O-succinyl-L-homoserine wild-type, pH 7.8, 25°C Escherichia coli
8
-
 O-succinyl-L-homoserine mutant N227A, pH 7.8, 25°C Escherichia coli
8
-
 O-succinyl-L-homoserine mutant R49K, pH 7.8, 25°C Escherichia coli
11
-
 O-succinyl-L-homoserine mutant R106A, pH 7.8, 25°C Escherichia coli
19
-
 O-succinyl-L-homoserine mutant D45A, pH 7.8, 25°C Escherichia coli
21
-
 O-succinyl-L-homoserine mutant S326A, pH 7.8, 25°C Escherichia coli
39
-
 O-succinyl-L-homoserine mutant D45N, pH 7.8, 25°C Escherichia coli
39
-
 O-succinyl-L-homoserine mutant R106K, pH 7.8, 25°C Escherichia coli
90
-
 O-succinyl-L-homoserine mutant Y46F, pH 7.8, 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-succinyl-L-homoserine + L-cysteine
-
 Escherichia coli L-cystathionine + succinate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 O-succinyl-L-homoserine mutant R48K, pH 7.8, 25°C Escherichia coli
0.31
-
 O-succinyl-L-homoserine mutant S326A, pH 7.8, 25°C Escherichia coli
0.5
-
 O-succinyl-L-homoserine mutant R361K, pH 7.8, 25°C Escherichia coli
1.4
-
 O-succinyl-L-homoserine mutant R106A, pH 7.8, 25°C Escherichia coli
5.3
-
 O-succinyl-L-homoserine mutant R106K, pH 7.8, 25°C Escherichia coli
5.6
-
 O-succinyl-L-homoserine mutant R49A, pH 7.8, 25°C Escherichia coli
6
-
 O-succinyl-L-homoserine mutant Y46F, pH 7.8, 25°C Escherichia coli
9.6
-
 O-succinyl-L-homoserine mutant Y101F, pH 7.8, 25°C Escherichia coli
33
-
 O-succinyl-L-homoserine mutant N227A, pH 7.8, 25°C Escherichia coli
57
-
 O-succinyl-L-homoserine mutant E325Q, pH 7.8, 25°C Escherichia coli
59
-
 O-succinyl-L-homoserine mutant E325A, pH 7.8, 25°C Escherichia coli
79
-
 O-succinyl-L-homoserine mutant D45A, pH 7.8, 25°C Escherichia coli
100
-
 O-succinyl-L-homoserine mutant D45N, pH 7.8, 25°C Escherichia coli
112
-
 O-succinyl-L-homoserine wild-type, pH 7.8, 25°C Escherichia coli
130
-
 O-succinyl-L-homoserine mutant R49K, pH 7.8, 25°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.002
-
 O-succinyl-L-homoserine mutant R48K, pH 7.8, 25°C Escherichia coli
0.005
-
 O-succinyl-L-homoserine mutant R361K, pH 7.8, 25°C Escherichia coli
0.015
-
 O-succinyl-L-homoserine mutant S326A, pH 7.8, 25°C Escherichia coli
0.071
-
 O-succinyl-L-homoserine mutant Y46F, pH 7.8, 25°C Escherichia coli
0.12
-
 O-succinyl-L-homoserine mutant R106A, pH 7.8, 25°C Escherichia coli
0.14
-
 O-succinyl-L-homoserine mutant R106K, pH 7.8, 25°C Escherichia coli
0.41
-
 L-cysteine mutant R106A, pH 7.8, 25°C Escherichia coli
0.68
-
 L-cysteine mutant R48K, pH 7.8, 25°C Escherichia coli
1.6
-
 L-cysteine mutant S326A, pH 7.8, 25°C Escherichia coli
2.3
-
 L-cysteine mutant R361K, pH 7.8, 25°C Escherichia coli
3.1
-
 O-succinyl-L-homoserine mutant R49A, pH 7.8, 25°C Escherichia coli
4.2
-
 O-succinyl-L-homoserine mutant N227A, pH 7.8, 25°C Escherichia coli
5.1
-
 O-succinyl-L-homoserine mutant Y101F, pH 7.8, 25°C Escherichia coli
8.2
-
 L-cysteine mutant R106K, pH 7.8, 25°C Escherichia coli
11.3
-
 L-cysteine mutant Y46F, pH 7.8, 25°C Escherichia coli
16
-
 O-succinyl-L-homoserine mutant R49K, pH 7.8, 25°C Escherichia coli
25
-
 O-succinyl-L-homoserine wild-type, pH 7.8, 25°C Escherichia coli
26
-
 O-succinyl-L-homoserine mutant D45N, pH 7.8, 25°C Escherichia coli
42
-
 O-succinyl-L-homoserine mutant D45A, pH 7.8, 25°C Escherichia coli
50
-
 O-succinyl-L-homoserine mutant E325Q, pH 7.8, 25°C Escherichia coli
50
-
 L-cysteine mutant R49A, pH 7.8, 25°C Escherichia coli
64
-
 L-cysteine mutant Y101F, pH 7.8, 25°C Escherichia coli
90
-
 O-succinyl-L-homoserine mutant E325A, pH 7.8, 25°C Escherichia coli
99
-
 L-cysteine mutant D45A, pH 7.8, 25°C Escherichia coli
110
-
 L-cysteine mutant N227A, pH 7.8, 25°C Escherichia coli
113
-
 L-cysteine mutant D45N, pH 7.8, 25°C Escherichia coli
200
-
 L-cysteine mutant E325Q, pH 7.8, 25°C Escherichia coli
350
-
 L-cysteine mutant E325A, pH 7.8, 25°C Escherichia coli
360
-
 L-cysteine mutant R49K, pH 7.8, 25°C Escherichia coli
470
-
 L-cysteine wild-type, pH 7.8, 25°C Escherichia coli