Information on EC 2.3.1.28 - chloramphenicol O-acetyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
2.3.1.28
-
RECOMMENDED NAME
GeneOntology No.
chloramphenicol O-acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate
show the reaction diagram
ternary complex mechanism with a rapid equilibrium and essentially random order of addition of substrates
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acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:chloramphenicol 3-O-acetyltransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
acetyltransferase, chloramphenicol
-
-
-
-
CAT
-
-
-
-
CAT
Q6XD71
catBcl gene, strain SIN
CAT I
-
-
-
-
CAT II
-
enzyme encoded by the incW transmissible plasmid
CAT III
-
-
-
-
CATI
P62577
-
chloramphenicol acetylase
-
-
-
-
chloramphenicol acetyltransferase
-
-
-
-
chloramphenicol acetyltransferase
Q6XD71
-
chloramphenicol acetyltransferase
-
-
chloramphenicol acetyltransferase
-
Tn9 cat gene
chloramphenicol acetyltransferase
-
-
chloramphenicol acetyltransferase
-
-
chloramphenicol acetyltransferase
Pseudomonas aeruginosa Pahcr
-
-
-
chloramphenicol acetyltransferase
-
-
chloramphenicol acetyltransferase B2
-
-
chloramphenicol transacetylase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9040-07-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
chloramphenicol resistant strains OC, NR, SIN, and T, susceptible control strains DSM8716, ATCC 21536, and ATCC 21537
UniProt
Manually annotated by BRENDA team
infected with either TE/3'2J/CAT or rep5/CAT/26S virus
-
-
Manually annotated by BRENDA team
CATII is encoded by the IncW transmissible plasmid pSA
-
-
Manually annotated by BRENDA team
chloramphenicol-resistant strains which carry the episomal resistance transfer factor R
-
-
Manually annotated by BRENDA team
enzyme form CAT III
-
-
Manually annotated by BRENDA team
enzyme is encoded ny tramsposon Tn2424
-
-
Manually annotated by BRENDA team
JM 109 with plasmid pCM1 containing the enzyme, K12 strains 47, 146, and 570 with chromosome location
-
-
Manually annotated by BRENDA team
multiresistance transposon Tn2424
-
-
Manually annotated by BRENDA team
R-factor-bearing strain W677/HJR 66
-
-
Manually annotated by BRENDA team
strain J53(R387)
-
-
Manually annotated by BRENDA team
strain W677/HJR66
-
-
Manually annotated by BRENDA team
Escherichia coli J53(R387)
strain J53(R387)
-
-
Manually annotated by BRENDA team
Escherichia coli W677/HJR66
strain W677/HJR66
-
-
Manually annotated by BRENDA team
Flavobacterium sp. CB60
CB60
-
-
Manually annotated by BRENDA team
enzymes encoded by plasmid pRI234, pMR375 and pMR385
-
-
Manually annotated by BRENDA team
enzyme is encoded by plasmid Rms418
-
-
Manually annotated by BRENDA team
strain PA 103
Uniprot
Manually annotated by BRENDA team
strain Pahcr, mutations G61S and 105C contribute synergistically to the Pahcr1 resistance phenotype
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa PA 103
strain PA 103
Uniprot
Manually annotated by BRENDA team
Pseudomonas aeruginosa Pahcr
strain Pahcr, mutations G61S and 105C contribute synergistically to the Pahcr1 resistance phenotype
-
-
Manually annotated by BRENDA team
encoded by plasmids pSCS6 or pSCS7
-
-
Manually annotated by BRENDA team
enzyme variant C and D
-
-
Manually annotated by BRENDA team
strain C22.1
-
-
Manually annotated by BRENDA team
Staphylococcus aureus C22.1
strain C22.1
-
-
Manually annotated by BRENDA team
enzyme variant B
-
-
Manually annotated by BRENDA team
enzyme type A
-
-
Manually annotated by BRENDA team
type A, type B and type C
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
-, Q6XD71
chloramphenicol resistance
physiological function
-
marker for chloramphenicol resistance
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
acetyl-CoA
-, Q6XD71
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
additional information
-
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
additional information
-
-
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
enzyme expression is increased at 42C. At 42C the enzyme is still soluble and active
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
45
-
30C: about 20% of maximal activity, 45C: about 55% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
Culex pipiens infected with either TE/3'2J/CAT or rep5/CAT/26S virus
Manually annotated by BRENDA team
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
loss of activity by freezing and thawing can be prevented by adding 10% glycerol
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20 C, activity in elution buffer is stable for at least 1 year
-
-20 C, stable for several months
-
-80 C, 20% glycerol, stable for more than 3 months
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