Protein Variants | Comment | Organism |
---|---|---|
additional information | Bacillus subtilis cells expressing a hybrid protein (LvsSS-Cat) consisting of the Bacillus amyloliquefaciens levansucrose signal peptide fused to Bacillus pumilus chloramphenicol acetyltransferase are unable to export cat protein into the growth medium. A series of tripartite protein fusion is constructed by inserting various length of the cat sequences between the levansucrase signal peptide and staphylococcal protein A or Escherichia coli alkaline phosphatase. Biochemical characterization of the various Cat protein fusion reveales that multiple regions in the cat protein are causing the export defect | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Bacillus subtilis | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + chloramphenicol | - |
Bacillus subtilis | CoA + chloramphenicol 3-acetate | - |
? |