Literature summary for 2.3.1.28 extracted from

Wang, J.; Liu, J.H.
Mutations in the chloramphenicol acetyltransferase (S61G, Y105C) increase accumulated amounts and resistance in Pseudomonas aeruginosa (2004), FEMS Microbiol. Lett., 236, 197-204.

Search for more literature for 2.3.1.28

Protein Variants

Protein Variants	Comment	Organism
G61S	G61S and Y105C contrtibute synergistically to the resistance phenotype of strain PAhcr1	Pseudomonas aeruginosa
G61S/Y105C	G61S and Y105C contrtibute synergistically to the resistance phenotype of strain PAhcr1	Pseudomonas aeruginosa
Y105C	G61S and Y105C contrtibute synergistically to the resistance phenotype of strain PAhcr1	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.795	-	chloramphenicol	mutant enzyme G61S/Y105C	Pseudomonas aeruginosa
0.797	-	acetyl-CoA	mutant enzyme G61S/Y105C	Pseudomonas aeruginosa
0.798	-	chloramphenicol	wild-type enzyme	Pseudomonas aeruginosa
0.8	11	chloramphenicol	mutant enzyme G61S	Pseudomonas aeruginosa
0.806	-	acetyl-CoA	mutant enzyme G61S	Pseudomonas aeruginosa
0.809	-	acetyl-CoA	mutant enzyme G61S/Y105C	Pseudomonas aeruginosa
0.809	-	chloramphenicol	mutant enzyme Y105C	Pseudomonas aeruginosa
0.82	-	acetyl-CoA	mutant enzyme Y105C	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	mutations G61S and 105C contribute synergistically to the Pahcr1 resistance phenotype	-
Pseudomonas aeruginosa Pahcr	-	mutations G61S and 105C contribute synergistically to the Pahcr1 resistance phenotype	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + chloramphenicol	-	Pseudomonas aeruginosa	CoA + chloramphenicol 3-acetate	-	?
acetyl-CoA + chloramphenicol	-	Pseudomonas aeruginosa Pahcr	CoA + chloramphenicol 3-acetate	-	?

Synonyms

Synonyms	Comment	Organism
chloramphenicol acetyltransferase	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)