Information on EC 1.7.2.1 - nitrite reductase (NO-forming)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY
1.7.2.1
-
RECOMMENDED NAME
GeneOntology No.
nitrite reductase (NO-forming)
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
ammonia oxidation II (anaerobic)
-
intra-aerobic nitrite reduction
-
Microbial metabolism in diverse environments
-
nitrate reduction I (denitrification)
-
nitrate reduction VII (denitrification)
-
nitrifier denitrification
-
Nitrogen metabolism
-
SYSTEMATIC NAME
IUBMB Comments
nitric-oxide:ferricytochrome-c oxidoreductase
The reaction is catalysed by two types of enzymes, found in the perimplasm of denitrifying bacteria. One type comprises proteins containing multiple copper centres, the other a heme protein, cytochrome cd1. Acceptors include c-type cytochromes such as cytochrome c-550 or cytochrome c-551 from Paracoccus denitrificans or Pseudomonas aeruginosa, and small blue copper proteins such as azurin and pseudoazurin. Cytochrome cd1 also has oxidase and hydroxylamine reductase activities. May also catalyse the reaction of hydroxylamine reductase (EC 1.7.99.1) since this is a well-known activity of cytochrome cd1.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AcNIR
Achromobacter cycloclastes IAM1013
-
-
-
AfNiR
Alcaligenes faecalis S-6
-
;
-
C551-O2 oxidoreductase
-
-
cd1 nitrite reductase
-
-
copper-containing dissimilatory nitrite reductase
P38501
-
copper-containing dissimilatory nitrite reductase
Alcaligenes faecalis S-6
P38501
-
-
copper-containing nitrite reductase
-
-
copper-containing nitrite reductase
P38501
-
copper-containing nitrite reductase
Alcaligenes faecalis S-6
P38501
-
-
copper-containing nitrite reductase
-
-
copper-containing nitrite reductase
Hyphomicrobium denitrificans A3151
-
-
-
cytochrome c-551:O2, NO2- oxidoreductase
-
-
-
-
cytochrome cd
-
-
-
-
cytochrome cd
-
-
cytochrome cd1 nitrite reductase
Q3LGA9
-
cytochrome cd1 nitrite reductase
Q3LGA9
-
-
cytochrome cd1 nitrite reductase
-
-
cytochrome cd1 nitrite reductase
P72181
-
cytochrome cd1 nitrite reductase
-
-
cytochrome oxidase
-
-
-
-
dissimilatory nitrite reductase cytochrome cd1
-
-
EC 1.9.3.2
-
formerly
HdNIR
Hyphomicrobium denitrificans A3151
-
-
-
hemoglobin
-
-
NiR
Alcaligenes faecalis S-6
P38501
-
-
NirK
A9XR52
-
NirK
uncultured bacterium KSU-1
-
-
-
nitrite reductase
Q9JYE1
-
nitrite reductase
P72181
-
nitrite reductase
-
-
nitrite reductase
P24040
-
nitrite reductase
Pseudomonas stutzeri ZoBell
P24040
-
-
oxidase, Pseudomonas cytochrome
-
-
-
-
PNR
Pseudomonas aeruginosa 5276
-
-
-
Pseudomonas cytochrome oxidase
-
-
-
-
Pseudomonas cytochrome oxidase
-
-
reductase, nitrite (cytochrome)
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9027-00-3
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Achromobacter cycloclastes IAM1013
IAM1013
-
-
Manually annotated by BRENDA team
Alcaligenes faecalis S-6
S-6
-
-
Manually annotated by BRENDA team
Alcaligenes faecalis S-6
strain S-6
UniProt
Manually annotated by BRENDA team
NCIB 11015
-
-
Manually annotated by BRENDA team
Hyphomicrobium denitrificans A3151
A3151
-
-
Manually annotated by BRENDA team
formerly Aquaspirillum magnetotacticum
-
-
Manually annotated by BRENDA team
wild-type strain MC58, gene nitrite reductase aniA
UniProt
Manually annotated by BRENDA team
formerly Micrococcus denitrificans
-
-
Manually annotated by BRENDA team
formerly Thiosphaera pantotropha
-
-
Manually annotated by BRENDA team
mutant H327A
-
-
Manually annotated by BRENDA team
strain 5276
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa 5276
strain 5276
-
-
Manually annotated by BRENDA team
Pseudomonas nautica 617
-
-
-
Manually annotated by BRENDA team
addition of nitrate to oxygen-limited cells induces cytochrome cd1 synthesis
-
-
Manually annotated by BRENDA team
strain ZoBell
-
-
Manually annotated by BRENDA team
strain ZoBell ATCC 14405
UniProt
Manually annotated by BRENDA team
Pseudomonas stutzeri ZoBell
strain ZoBell
-
-
Manually annotated by BRENDA team
Pseudomonas stutzeri ZoBell
strain ZoBell ATCC 14405
UniProt
Manually annotated by BRENDA team
f. sp. denitrificans
-
-
Manually annotated by BRENDA team
strain 2.4.3
-
-
Manually annotated by BRENDA team
uncultured bacterium KSU-1
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
A4TUE6, -
deletion of cytochrome cd1-type nitrite reductase NirS gene or gene NirN results in impaired growth and smaller, fewer, and aberrantly shaped magnetite crystals during nitrate reduction. Nitrite reduction is completely abolished in the DELTAnirS mutant. NirN is required for full reductase activity of NirS by maintaining a proper form of d1 heme for holo-cytochrome cd1 assembly
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
6.5
-
assay at, pH dependence, overview
5.1
5.4
-
hydroxylamine reduction, electron donor pseudoazurin, pre-reduced cytochrome cd1; NH2OH reduction, electron donor pseudoazurin
5.4
-
-
hydroxylamine reduction, electron donor cytochrome c550, pre-reduced cytochrome cd1; NH2OH reduction, electron donor cytochrome c550
5.6
-
-
reaction with 0.5 mM nitrite and pseudoazurin as electron donor
5.7
-
-
nitrite reduction, electron donor cytochrome c550; nitrite reduction, electron donor cytochrome c550, pre-reduced cytochrome cd1
5.8
-
-
nitrite reduction, electron donor pseudoazurin; nitrite reduction, electron donor pseudoazurin, pre-reduced cytochrome cd1
6
-
-
reaction with 5 mM nitrite and pseudoazurin as electron donor
6.2
-
-
O2 reduction, electron donor cytochrome c550; O2 reduction, electron donor cytochrome c550, pre-reduced cytochrome cd1
6.3
-
-
O2 reduction, electron donor pseudoazurin; O2 reduction, electron donor pseudoazurin, pre-reduced cytochrome cd1
6.5
7
-
cytochrome c oxidase activity
6.5
-
A9XR52
optimum below pH 6.5
6.5
-
P24040
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.9
6.2
-
pH 4.9: about 55% of maximal ativity, pH 6.2: about 65% of maximal activity, reaction with 0.5 mM nitrite and pseudoazurin as electron donor
5.2
6.5
-
pH 5.2: about 60% of maximal activity, pH 6.5: about 55% of maximal activity, reaction with 5 mM nitrite and pseudoazurin as electron donor
additional information
-
P24040
almost no activity at pH 9.0
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
-
assay at
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
60
80
-, Q3LGA9
60°C: about 60% of maximal activity, 80°C: about 90% of maximal activity
PDB
SCOP
CATH
ORGANISM
Alcaligenes xylosoxydans xylosoxydans
Alcaligenes xylosoxydans xylosoxydans
Alcaligenes xylosoxydans xylosoxydans
Alcaligenes xylosoxydans xylosoxydans
Alcaligenes xylosoxydans xylosoxydans
Alcaligenes xylosoxydans xylosoxydans
Alcaligenes xylosoxydans xylosoxydans
Alcaligenes xylosoxydans xylosoxydans
Alcaligenes xylosoxydans xylosoxydans
Alcaligenes xylosoxydans xylosoxydans
Alcaligenes xylosoxydans xylosoxydans
Alcaligenes xylosoxydans xylosoxydans
Alcaligenes xylosoxydans xylosoxydans
Alcaligenes xylosoxydans xylosoxydans
Alcaligenes xylosoxydans xylosoxydans
Alcaligenes xylosoxydans xylosoxydans
Alcaligenes xylosoxydans xylosoxydans
Pseudoalteromonas haloplanktis (strain TAC 125)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Ralstonia pickettii (strain 12J)
Ralstonia pickettii (strain 12J)
Ralstonia pickettii (strain 12J)
Ralstonia pickettii (strain 12J)
Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)