Cloned (Comment) | Organism |
---|---|
expression in Haloferax volcanii, intracellular enzyme | Haloferax mediterranei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
reduced methyl viologen | pH and temperature not specified in the publication | Haloferax mediterranei | |
4.04 | - |
nitrite | pH and temperature not specified in the publication | Haloferax mediterranei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | important differences in the primary structure of the cytoplasmic enzyme form and the extracellularv enzyme form, indicating that Haloferax mediterranei could carry out a maturation and exportation process within the cell before the protein is exported to the S-layer. Several conserved signals found in Cu-NirK from Haloferax mediterranei sequence indicate that these processes are closely related to the Tat system | Haloferax mediterranei | 5737 | - |
extracellular | important differences in the primary structure of the cytoplasmic enzyme form and the extracellularv enzyme form, indicating that Haloferax mediterranei could carry out a maturation and exportation process within the cell before the protein is exported to the S-layer. Several conserved signals found in Cu-NirK from Haloferax mediterranei sequence indicate that these processes are closely related to the Tat system | Haloferax mediterranei | - |
- |
membrane | - |
Haloferax mediterranei | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | optimum salt concentration: 2 M | Haloferax mediterranei | |
NaCl | optimum salt concentration: 2 M | Haloferax mediterranei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39800 | - |
3 * 39800, SDS-PAGE, intracellular enzyme | Haloferax mediterranei |
44300 | - |
3 * 44300, SDS-PAGE, extracellular enzyme | Haloferax mediterranei |
103000 | - |
gel filtration, intracellular enzyme | Haloferax mediterranei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax mediterranei | D0RAY2 | - |
- |
Haloferax mediterranei DSM 1411 | D0RAY2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloferax mediterranei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Haloferax mediterranei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + reduced methyl viologen | - |
Haloferax mediterranei | NO + oxidized methyl viologen + H2O | - |
? | |
nitrite + reduced methyl viologen | - |
Haloferax mediterranei DSM 1411 | NO + oxidized methyl viologen + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 39800, SDS-PAGE, intracellular enzyme | Haloferax mediterranei |
trimer | 3 * 44300, SDS-PAGE, extracellular enzyme | Haloferax mediterranei |
Synonyms | Comment | Organism |
---|---|---|
Cu-NirK | - |
Haloferax mediterranei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Haloferax mediterranei |