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Literature summary for 1.7.2.1 extracted from
- Nitrite reductase from the magnetotactic bacterium Magnetospirillum magnetotacticum. A novel cytochrome cd1 with Fe(II):nitrite oxidoreductase activity (1995), Eur. J. Biochem., 233, 665-671.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CN- | 0.047 mM, 50% inhibition of nitrite reduction | Magnetospirillum magnetotacticum |  |
| CN- | - |
Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00147 | - |
NO2- | - |
Magnetospirillum magnetotacticum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Pseudomonas aeruginosa | - |
- |
| periplasm | 75% of enzyme activity found in the soluble fraction | Magnetospirillum magnetotacticum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
2 * 54000, SDS-PAGE | Magnetospirillum magnetotacticum |
| 133000 | - |
gel filtration | Magnetospirillum magnetotacticum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Magnetospirillum magnetotacticum | - |
formerly Aquaspirillum magnetotacticum | - |
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Toyopearl, CM-cellulose, Sephacryl S-200 | Magnetospirillum magnetotacticum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.32 | - |
reduction of N,N,N',N'-tetramethyl-4-phenyldiamine | Magnetospirillum magnetotacticum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ferrocytochrome c-551 + O2 | - |
Pseudomonas aeruginosa | H2O + ferricytochrome c-551 | - |
? | |
| ferrocytochrome c-551 + O2 | - |
Magnetospirillum magnetotacticum | H2O + ferricytochrome c-551 | - |
? | |
| reduced tetramethyl-4-phenylenediamine + NO2 | no reaction with horse ferrocytochrome c, Neurospora europaea ferrocytochrome c-552, Magnetospirillum magnetotacticum ferrocytochrome c-550 and Pseudomonas aeruginosa cytochrome c-551 | Magnetospirillum magnetotacticum | oxidized tetrametyl-4-phenylenediamine + NO | - |
? | |
| reduced tetramethyl-4-phenylenediamine + O2 | - |
Magnetospirillum magnetotacticum | oxidized tetrametyl-4-phenylenediamine + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Pseudomonas aeruginosa |
| dimer | 2 * 54000, SDS-PAGE | Magnetospirillum magnetotacticum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 80 | - |
NO2- | - |
Pseudomonas aeruginosa | |
| 80 | - |
NO2- | electron donor: reduced tetramethyl-4-phenylenediamine | Magnetospirillum magnetotacticum | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme c | - |
Pseudomonas aeruginosa | |
| heme c | - |
Magnetospirillum magnetotacticum | |
| Heme d1 | - |
Pseudomonas aeruginosa | |
| Heme d1 | - |
Magnetospirillum magnetotacticum | |
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Release 2023.1 (January 2023)
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