Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and in HEK cell | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C273A | mutation in the putative active site cysteine residue, known to coordinate molybdenum binding. NO formation is abolished by the C273A mutation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Tungsten | replacement of molybdenum by tungsten abolishes NO formation | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.5 | - |
nitrite | pH 7.4, temperature not specified in the publication | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | nitrite reduction is catalyzed by molybdenum in the active site | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q5VT66 | - |
- |
Homo sapiens | Q969Z3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | mARC can generate nitric oxide from nitrite when forming an electron transfer chain with NADH, cytochrome b5, and NADH-dependent cytochrome b5 reductase | Homo sapiens | ? | - |
? | |
nitrite + ferrocytochrome b5 + 2 H+ | - |
Homo sapiens | nitric oxide + H2O + ferricytochrome b5 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE, recombinant His-tagged protein lacking the mitochondrial translocation motif | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
mARC1 | - |
Homo sapiens |
mARC2 | - |
Homo sapiens |
mitochondrial amidoxime reducing component | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
nitrite | pH 7.4, temperature not specified in the publication | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
3fold decrease in the rate of NO formation compared to pH 6.5 | Homo sapiens |