Information on EC 1.6.5.10 - NADPH dehydrogenase (quinone)

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
1.6.5.10
-
RECOMMENDED NAME
GeneOntology No.
NADPH dehydrogenase (quinone)
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
NADPH + H+ + a quinone = NADP+ + a quinol
show the reaction diagram
-
-
-
-
NADPH + H+ + a quinone = NADP+ + a quinol
show the reaction diagram
ping pong reaction mechanism
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
NADPH:(quinone-acceptor) oxidoreductase
A flavoprotein [1, 2]. The enzyme from Escherichia coli is specific for NADPH and is most active with quinone derivatives and ferricyanide as electron acceptors [3]. Menaquinone can act as acceptor. The enzyme from hog liver is inhibited by dicoumarol and folic acid derivatives but not by 2,4-dinitrophenol [1].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AKR1C9
P23457
-
CBR4
-
-
dehydrogenase, reduced nicotinamide adenine dinucleotide phosphate (quinone)
-
-
-
-
NADPH oxidase
-
-
-
-
NADPH oxidase
-
-
NADPH oxidase
-
-
NADPH quinone oxidoreductase
Q8NKK9
-
NADPH quinone oxidoreductase 1
-
-
NADPH quinone reductase
-
-
NADPH quinone reductase
Helicobacter hepaticus ATCC51449
-
-
-
NADPH quinone reductase
-
-
NADPH-dependent quinone reductase
-
-
NADPH-quinone oxidoreductase
-
-
NADPH:quinone oxidoreductase 1
-
-
NADPH:quinone oxidoreductase 1
-
-
NQO1
-
-
NQO1
-
-
MdaB
Helicobacter hepaticus ATCC51449
-
-
-
additional information
P23457
bifunctional 3alpha-hydroxysteroid dehydrogenase and NADPH reductase (quinone)
CAS REGISTRY NUMBER
COMMENTARY
37256-37-4
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain ATCC51449
-
-
Manually annotated by BRENDA team
Helicobacter hepaticus ATCC51449
strain ATCC51449
-
-
Manually annotated by BRENDA team
urothelial carcinoma patients
-
-
Manually annotated by BRENDA team
Wistar rats
-
-
Manually annotated by BRENDA team
hog
-
-
Manually annotated by BRENDA team
gene arsH
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
evolution
-
the enzyme is a member of the family of NADPH-dependent FMN reductases
physiological function
-
presence of Zta1p has an in vivo protective effect on yeast strains exposed to the toxic substrate 3-penten-2-one
physiological function
-
ArsH plays a role in the response to oxidative stress caused by arsenite
malfunction
-
arsH mutants are sensitive to the oxidizing agent menadione
additional information
-
although the enzyme is able to stabilize the anionic semiquinone form of the FMN, reduction of quinones involves the hydroquinone form of the flavin cofactor, and the enzymatic reaction occurs through a ping pong-type mechanism. ArsH is able to catalyze one-electron reactions (oxygen and cytocrome c reduction), involving the FMN semiquinone form, but with lower efficiency
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R,S)-camphorquinone + NADPH + H+
(R,S)-camphorquinone + NADP+
show the reaction diagram
P23457
-
-
-
?
1,2-naphthoquinone + NADPH + H+
1,2-naphthoquinol + NADP+
show the reaction diagram
-
-
-
-
?
1,2-naphthoquinone + NADPH + H+
1,2-naphthoquinol + NADP+
show the reaction diagram
-
-
-
-
?
1,4-benzoquinone + NADPH + H+
1,4-benzoquinol + NADP+
show the reaction diagram
-
1,4-benzoquinone is oxidized almost stoichiometric amount of NADPH, suggesting that it is reduced to its hydroquinone with a two-electron reduction mechanism
-
-
?
1,4-benzoquinone + NADPH + H+
1,4-benzoquinol + NADP+
show the reaction diagram
-
about 81% of the activity with menadione
-
-
?
1,4-naphthoquinone + NADPH + H+
1,4-naphthoquinol + NADP+
show the reaction diagram
-
specific for NADPH
-
-
?
1,4-naphthoquinone + NADPH + H+
1,4-naphthoquinol + NADP+
show the reaction diagram
-
1,4-naphthoquinone participates in the redox cycling in their reduction by this enzyme as evidenced by excess NADPH oxidation over quinone reduction. CBR4 may reduce 1,4-naphthoquinone in the two-electron reduction mechanism, but produces reactive superoxide and semiquinones through their redox cycling
-
-
?
1,4-naphthoquinone + NADPH + H+
1,4-naphthoquinol + NADP+
show the reaction diagram
-
two-electron transfer mechanism from NADPH to quinone
-
-
?
1,4-naphthoquinone + NADPH + H+
1,4-naphthoquinol + NADP+
show the reaction diagram
-
about 85% of the activity with menadione
-
-
?
2,3-dimethoxy-5-methyl-1,4-benzoquinone + NADPH + H+
2,3-dimethoxy-5-methyl-1,4-benzoquinol + NADP+
show the reaction diagram
-
-
-
-
?
2,6-dichlorophenolindophenol + NADPH + H+
reduced 2,6-dichlorophenolindophenol + NAD(P)+
show the reaction diagram
-
very low activity with NADH
-
-
?
2-methyl-1,4-naphthoquinone + NADPH + H+
2-methyl-1,4-naphthoquinol + NADP+
show the reaction diagram
-
-
-
-
?
2-methyl-1,4-naphthoquinone + NADPH + H+
2-methyl-1,4-naphthoquinol + NADP+
show the reaction diagram
-
i.e. menadione, specific for NADPH
-
-
?
2-methyl-5-hydroxy-1,4-naphthoquinone + NADPH + H+
2-methyl-5-hydroxy-1,4-naphthoquinol + NADP+
show the reaction diagram
-
-
-
-
?
5-hydroxy-1,4-naphthoquinone + NADPH + H+
5-hydroxy-1,4-naphthoquinol + NADP+
show the reaction diagram
-
-
-
-
?
5-hydroxy-1,4-naphthoquinone + NADPH + H+
5-hydroxy-1,4-naphthoquinol + NADP+
show the reaction diagram
-
5-hydroxy-1,4-naphthoquinone participates in the redox cycling in their reduction by this enzyme as evidenced by excess NADPH oxidation over quinone reduction
-
-
?
5-hydroxy-2-methyl-1,4-naphthoquinone + NADPH + H+
5-hydroxy-2-methyl-1,4-naphthoquinol + NADP+
show the reaction diagram
-
-
-
-
?
9,10-phenanthrenequinone + NADPH + H+
9,10-phenanthrenequinol + NADP+
show the reaction diagram
-
-
-
-
?
9,10-phenanthrenequinone + NADPH + H+
9,10-phenanthrenequinol + NADP+
show the reaction diagram
P23457
-
-
-
?
9,10-phenanthrenequinone + NADPH + H+
9,10-phenanthrenequinol + NADP+
show the reaction diagram
-
9,10-phenanthrenequinone participates in the redox cycling in their reduction by this enzyme as evidenced by excess NADPH oxidation over quinone reduction. CBR4 may reduce 9,10-phenanthrenequinone in the two-electron reduction mechanism, but produces reactive superoxide and semiquinones through their redox cycling
-
-
?
acenaphthenequinone + NADPH + H+
acenaphthenequinol + NADP+
show the reaction diagram
P23457
-
-
-
?
benzoquinone + NADPH
benzohydroquinone + NADP+
show the reaction diagram
-
specific for NADPH
-
-
?
dichlorophenolindophenol + NADPH + H+
? + NADP+
show the reaction diagram
-
about 15% of the activity with menadione
-
-
?
ferricyanide + NADPH + H+
ferrocyanide + NADP+
show the reaction diagram
-
-
-
-
?
ferricyanide + NADPH + H+
ferrocyanide + NADP+
show the reaction diagram
-
about 90% of the activity with menadione
-
-
?
menadione + NADPH + H+
? + NADP+
show the reaction diagram
-
two-electron transfer mechanism from NADPH to quinone
-
-
?
menadione + NADPH + H+
menadiol + NADP+
show the reaction diagram
-
-
-
-
?
menadione + NADPH + H+
menadiol + NADP+
show the reaction diagram
-
-
-
-
?
NADH + acceptor
NADH + reduced acceptor
show the reaction diagram
-
no activity
-
-
-
NADH + Fe(CN)63-
NAD+ + Fe(CN)63-
show the reaction diagram
-
with NADH the actuvity is less than 10% of the activity with NADPH
-
-
?
NADH + H+ + 1,4-naphthoquinone
NAD+ + 1,4-naphthoquinol
show the reaction diagram
-
with NADH the activity is about 15% of the activity with NADPH
-
-
?
NADH + H+ + coenzyme Q0
NAD+ + reduced coenzyme Q0
show the reaction diagram
-
with NADH the activity is less than 10% of the activity with NADPH
-
-
?
NADH + H+ + coenzyme Q1
NAD+ + reduced coenzyme Q1
show the reaction diagram
-
with NADH the activity is less than 10% of the activity with NADPH
-
-
?
NADH + H+ + dichlorophenolindophenol
NAD+ + reduced dichlorophenolindophenol
show the reaction diagram
-
with NADH the actuvity is about 15% of the activity with NADPH
-
-
?
NADH + menadione
NAD+ + ?
show the reaction diagram
-
with NADH the activity is less than 10% of the activity with NADPH
-
-
?
NADPH + acceptor
NADP+ + reduced acceptor
show the reaction diagram
-
acceptor: ferricyanide, acceptor: menadione, acceptor: benzoquinone, acceptor: ferric chloride, acceptor: methylene blue, acceptor: FMN, aceptor: 1,10-phenanthroline, 2,3',6-trichlorophenolindophenol is reduced to leuco-2,3',6-trichlorophenolindophenol
-
-
?
NADPH + ferricyanide
NADP+ + ferrocyanide
show the reaction diagram
-
-
-
-
?
NADPH + H+ + 1,4-benzoquinone
NADP+ + 1,4-benzohydroquinol
show the reaction diagram
-, Q8NKK9
no activity with NADH
-
-
?
NADPH + H+ + 1,4-naphthoquinone
NADP+ + 1,4-naphthohydroquinone
show the reaction diagram
-
-
-
?
NADPH + H+ + 1,4-naphthoquinone
NADP+ + 1,4-naphthoquinol
show the reaction diagram
-
-
-
-
?
NADPH + H+ + 2,3-dichloro-5,6-dicyano-1,4-benzoquinone
NADP+ + 2,3-dichloro-5,6-dicyano-1,4-benzoquinol
show the reaction diagram
-, Q8NKK9
-
-
-
?
NADPH + H+ + 2,5-dimethyl-4-benzoquinone
NADP+ + 2,5-dimethyl-4-benzoquinonl
show the reaction diagram
-
-
-
-
?
NADPH + H+ + 2,6-dichlorophenolindophenol
NADP+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
NADPH + H+ + 2-hydroxy-1,4-naphthoquinone
NADP+ + 2-hydroxy-1,4-naphthoquinol
show the reaction diagram
-
-
-
-
?
NADPH + H+ + a quinone
NADP+ + a quinol
show the reaction diagram
-
the catalytic cycle of ArsH consists of the acceptance of two electrons from NADPH to reduce the flavin cofactor (reductive half-reaction) and the transfer of these electrons to an acceptor (oxidative half-reaction)
-
-
?
NADPH + H+ + anthraquinone-2-sulfonate
NADP+ + ?
show the reaction diagram
-
-
-
-
?
NADPH + H+ + coenzyme Q0
NADP+ + reduced coenzyme Q0
show the reaction diagram
-
-
-
-
?
NADPH + H+ + coenzyme Q1
NADP+ + reduced coenzyme Q1
show the reaction diagram
-
-
-
-
?
NADPH + H+ + coenzyme Q10
NADP+ + reduced coenzyme Q10
show the reaction diagram
-
-
-
-
?
NADPH + H+ + cytochrome c
NADP+ + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
NADPH + H+ + dibromothymoquinone
NADP+ + dibromothymoquinol
show the reaction diagram
-
-
-
-
?
NADPH + H+ + dichlorophenolindophenol
NADP+ + reduced dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
NADPH + H+ + duroquinone
NADP+ + durohydroquinone
show the reaction diagram
-
-
-
?
NADPH + H+ + duroquinone
NADP+ + duroquinol
show the reaction diagram
-
-
-
-
?
NADPH + H+ + duroquinone
NADP+ + duroquinol
show the reaction diagram
-
-
-
-
?
NADPH + H+ + menadione
NADP+ + menadiol
show the reaction diagram
-
-
-
?
NADPH + H+ + menadione
NADP+ + menadiol
show the reaction diagram
-
-
-
-
?
NADPH + H+ + menadione
NADP+ + ?
show the reaction diagram
-
-
-
-
?
NADPH + H+ + menadione
NADP+ + reduced menadiol
show the reaction diagram
-
-
-
-
?
NADPH + H+ + methyl-p-benzoquinone
NADP+ + methyl-p-benzohydroquinone
show the reaction diagram
-
-
-
?
NADPH + H+ + p-benzoquinone
NADP+ + p-benzohydroquinone
show the reaction diagram
-
-
-
?
NADPH + H+ + phenyl-1,4-benzoquinone
NADP+ + phenyl-1,4-benzoquinol
show the reaction diagram
-, Q8NKK9
-
-
-
?
NADPH + H+ + quinone
NADP+ + quinol
show the reaction diagram
-, Q8NKK9
-
-
-
?
NADPH + H+ + quinone
NADP+ + ?
show the reaction diagram
-
TNF alpha and LPS induce Nqo1 mRNA expression
-
-
?
NADPH + H+ + ubiquinone-1
NADP+ + ubihydroquinone-1
show the reaction diagram
-
-
-
?
oxidized dichlorophenolindophenol + NADPH + H+
reduced dicholorophenolindophenol + NADP+
show the reaction diagram
-
-
-
-
?
ubiquinone-0 + NADPH + H+
ubiquinol-0 + NADP+
show the reaction diagram
-
two-electron transfer mechanism from NADPH to quinone
-
-
?
ubiquinone-1 + NADPH + H+
ubiquinol-1 + NADP+
show the reaction diagram
-
two-electron transfer mechanism from NADPH to quinone
-
-
?
menadione + NADPH + H+
menadiol + NADP+
show the reaction diagram
-
reduction follows a two-electron-pathway
-
-
?
additional information
?
-
-
the enzyme plays an important role in managing oxidative stress and contributes to successful colonization of the host
-
-
-
additional information
?
-
-
the enzyme exhibits NADPH-dependent reductase activity for o- and p-quinones, but not for other aldehydes and ketones. In vitro quinone reduction by CBR4 generates superoxide through the redox cycling, and suggest that the enzyme may be involved in the induction of apoptosis by cytotoxic 9,10-phenanthrenequinone
-
-
-
additional information
?
-
P23457
enzyme displays bifunctional 3alpha-hydroxysteroid dehydrogenase and NADPH reductase (quinone) activities. Quinone reduction occurs via a mechanism that differs from 3-ketosteroid reduction. In this mechanism, the electron donor NADPH and acceptor o-quinone are bound in close proximity, which permits hydride transfer without formal protonation of the acceptor carbonyl by Tyr 55
-
-
-
additional information
?
-
-
trace activity with substrates acrolein, cinnamaldehyde, 3-buten-2-one, 3-penten-2-one, 3-nonen-2-one
-
-
-
additional information
?
-
-
NADH, deazariboflavin, and methylviologen can also act as electron donors
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NADPH + H+ + a quinone
NADP+ + a quinol
show the reaction diagram
-
the catalytic cycle of ArsH consists of the acceptance of two electrons from NADPH to reduce the flavin cofactor (reductive half-reaction) and the transfer of these electrons to an acceptor (oxidative half-reaction)
-
-
?
NADPH + H+ + cytochrome c
NADP+ + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the enzyme plays an important role in managing oxidative stress and contributes to successful colonization of the host
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
cytochrome b558
-
gp91phox and p22phox are integral membrane proteins that form a heterodimeric flavocytochrome b558, the catalytic core of the enzyme
-
cytochrome c
-
-
FAD
-
one FAD per enzyme molecule of 35000 Da
FAD
-
activity depends on added FAD
FAD
-
enzyme displays flavin absorption spectrum with peaks at 376 and 456 nm and a shoulder at around 484 nm. Protein caontains FAD
flavin
-
flavoprotein, adsorption spectrum with a major peak at 456 nm
flavin
-
the enzyme is a flavoprotein
FMN
-
bound non-covalently at the COOH termini of the beta-sheet
NADH
-
activity is 15% or less of the activity with NADPH
NADPH
-
very low activity with NADH
NADPH
-, Q8NKK9
no activity with NADH
NADPH
-
NADH could also be used as an electron donor to MdaB but with much lower efficiency, about 3% of those with NADPH
NADPH
-
specific
additional information
-
no cofactor: NADH, FMN
-
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(N-methyl-4'-pyridyl) porphyrin
-
iron tetrakis, FeTMPyP, no inhibitory activity
1,10-phenanthroline
-
1 mM, 72% inhibition
1-(2-thenoyl)-3,3,3-trifluoracetone
-
1 mM, 89% inhibition
2,4-Dinitrophenol
-
1 mM, 86% inhibition
4-(2-aminoethyl)benzenesulfonylfluoride
-
-
4-(2-aminoethyl)benzenesulfonylfluoride
-
0.206 mM, 50% inhibition, noncompetitive vs. NADPH, competitive vs. p47-phox in the concentration range of 0.5-2.0 mM
4-(amidino)benzenesulfonylfluoride
-
1.49 mM, 50% inhibition
8-hydroxyquinoline
-
0.3 mM, 80% inhibition
ATP
-
0.2 mM, 75% inhibition
Cibacron blue F3GA
-
the 1,4-naphthoquinone activity of CBR4 is potently inhibited, competitive with respect to NADPH
dicoumarol
-
-
diphenylene iodonium
-
inhibition of the NADPH oxidase complex activity reduces the increase of superoxide production induced by palmitic acid
diphenyleneiodonium chloride
-
a flavoprotein inhibitor
EDTA
-
0.15 mM, 80% inhibition
Folic acid derivatives
-
-
iodoacetate
-
3 mM
NADP+
-
the 1,4-naphthoquinone activity of CBR4 is potently inhibited, competitive with respect to NADPH
NADP+
-
NADP+ acts as a competitive inhibitor for NADPH binding at the active site of an enzyme
Procion red HE3B
-
the 1,4-naphthoquinone activity of CBR4 is potently inhibited, competitive with respect to NADPH
-
L-N6-(1-iminoethyl) lysine
-
no inhibitory activity
additional information
-
not inhibited by 2,4-dinitrophenol
-
additional information
-
the enzyme is insensitive to dicuomarol and quercetin, potent inhibitors of cytosolic quinone reductases
-
additional information
-
insensitive to 4-chloromercuribenzoate at 100 microM, heavy metals such as Cu2+, Co2+, Cd2+ and Mn2+ at 10 microM each, and chelating agents such as EDTA, o-phenanthroline and diethyldithiocarbamate at 100 microM each
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-methylen-4-butyrolactone
-
25fold induction
-
beta-Naphthoflavone
-
-
lithiumdodecylsulfate
-
-
additional information
-
palmitic acid and antimycin A induce the enzyme and superoxide production
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.003
-
1,2-naphthoquinone
-
pH 7.4, 25C
0.009
-
1,2-naphthoquinone
-
pH 7.0, temperature not specified in the publication
0.0019
-
1,4-benzoquinone
-
pH 7.4, 25C
1.8
-
1,4-benzoquinone
-, Q8NKK9
25C, pH 6.5
0.0065
-
1,4-Naphthoquinone
-
pH 7.4, 25C
298.2
-
2,3-dichloro-5,6-dicyano-1,4-benzoquinone
-, Q8NKK9
25C, pH 6.5
0.0044
-
2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
pH 7.4, 25C
0.0013
-
2,5-dimethyl-4-benzoquinone
-
pH 8.0, 25C, recombinant His-tagged enzyme
-
0.0237
-
2-Hydroxy-1,4-naphthoquinone
-
pH 8.0, 25C, recombinant His-tagged enzyme
0.003
-
2-methyl-1,4-naphthoquinone
-
pH 7.4, 25C
0.0045
-
5-hydroxy-1,4-naphthoquinone
-
pH 7.4, 25C
0.0043
-
5-Hydroxy-2-methyl-1,4-naphthoquinone
-
pH 7.4, 25C
0.0016
-
9,10-phenanthrenequinone
-
pH 7.4, 25C
0.002
-
9,10-phenanthrenequinone
P23457
wild-type, pH 6.0, 25C
0.004
-
9,10-phenanthrenequinone
-
pH 7.0, temperature not specified in the publication
0.012
-
9,10-phenanthrenequinone
P23457
mutant D50E, pH 6.0, 25C
0.014
-
9,10-phenanthrenequinone
P23457
mutant Y55F, pH 6.0, 25C
0.026
-
9,10-phenanthrenequinone
P23457
mutant Y55S, pH 6.0, 25C
0.044
-
9,10-phenanthrenequinone
P23457
mutant H117A, pH 6.0, 25C
0.056
-
9,10-phenanthrenequinone
P23457
mutant D50N, pH 6.0, 25C
0.0345
-
anthraquinone-2-sulfonate
-
pH 8.0, 25C, recombinant His-tagged enzyme
0.0119
-
coenzyme Q10
-
pH 8.0, 25C, recombinant His-tagged enzyme
0.0012
-
dibromothymoquinone
-
pH 8.0, 25C, recombinant His-tagged enzyme
0.0028
-
duroquinone
-
pH 8.0, 25C, recombinant His-tagged enzyme
0.019
-
duroquinone
-
pH 7.5, 25C
647.5
-
hydroquinone
-, Q8NKK9
25C, pH 6.5
0.0057
-
menadione
-
pH 8.0, 25C, recombinant His-tagged enzyme
0.006
-
menadione
-
pH 7.5, 30C
0.011
-
menadione
-
-
0.006
-
NADPH
-
-
0.0105
-
NADPH
-
pH 7.5, 30C
0.0315
-
NADPH
-
pH 8.0, 25C, recombinant His-tagged enzyme
0.046
-
NADPH
-
-
728.6
-
phenyl-1,4-benzoquinone
-, Q8NKK9
25C, pH 6.5
0.036
-
menadione
-
pH 7.4, 25C
additional information
-
additional information
-
stopped-flow and steady-state kinetics, overview
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.21
-
1,2-naphthoquinone
-
pH 7.4, 25C
4.2
-
1,2-naphthoquinone
-
pH 7.0, temperature not specified in the publication
0.207
-
1,4-benzoquinone
-
pH 7.4, 25C
15833
-
1,4-benzoquinone
-, Q8NKK9
25C, pH 6.5
0.195
-
1,4-Naphthoquinone
-
pH 7.4, 25C
520
-
2,3-dichloro-5,6-dicyano-1,4-benzoquinone
-, Q8NKK9
25C, pH 6.5
0.068
-
2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
pH 7.4, 25C
36.7
-
2,5-dimethyl-4-benzoquinone
-
pH 8.0, 25C, recombinant His-tagged enzyme
-
11
-
2-Hydroxy-1,4-naphthoquinone
-
pH 8.0, 25C, recombinant His-tagged enzyme
0.19
-
5-hydroxy-1,4-naphthoquinone
-
pH 7.4, 25C
0.01
-
9,10-phenanthrenequinone
P23457
mutant D50N, pH 6.0, 25C
0.02
-
9,10-phenanthrenequinone
P23457
mutant D50E, pH 6.0, 25C
0.04
-
9,10-phenanthrenequinone
P23457
mutant H117A, pH 6.0, 25C
0.305
-
9,10-phenanthrenequinone
-
pH 7.4, 25C
1.45
-
9,10-phenanthrenequinone
P23457
mutant Y55F, pH 6.0, 25C; mutant Y55S, pH 6.0, 25C
2.85
-
9,10-phenanthrenequinone
P23457
wild-type, pH 6.0, 25C
4.2
-
9,10-phenanthrenequinone
-
pH 7.0, temperature not specified in the publication
9.4
-
anthraquinone-2-sulfonate
-
pH 8.0, 25C, recombinant His-tagged enzyme
5
-
coenzyme Q10
-
pH 8.0, 25C, recombinant His-tagged enzyme
35
-
dibromothymoquinone
-
pH 8.0, 25C, recombinant His-tagged enzyme
2.67
-
duroquinone
-
pH 7.5, 25C
25
-
duroquinone
-
pH 8.0, 25C, recombinant His-tagged enzyme
1600
-
hydroquinone
-, Q8NKK9
25C, pH 6.5
0.212
-
menadione
-
pH 7.4, 25C
3.04
-
menadione
-
pH 7.5, 25C
26
-
menadione
-
pH 8.0, 25C, recombinant His-tagged enzyme
360
-
phenyl-1,4-benzoquinone
-, Q8NKK9
25C, pH 6.5
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
462
-
1,2-naphthoquinone
-
pH 7.0, temperature not specified in the publication
678
0.06
-
9,10-phenanthrenequinone
P23457
mutant Y55S, pH 6.0, 25C
6039
0.1
-
9,10-phenanthrenequinone
P23457
mutant Y55F, pH 6.0, 25C
6039
1.43
-
9,10-phenanthrenequinone
P23457
wild-type, pH 6.0, 25C
6039
1041
-
9,10-phenanthrenequinone
-
pH 7.0, temperature not specified in the publication
6039
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.001
-
Cibacron blue F3GA
-
pH 7.4, 25C
0.057
-
NADP+
-
pH 7.4, 25C
0.0003
-
Procion red HE3B
-
pH 7.4, 25C
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.008
0.01
dicoumarol
-
with dichlorophenolindophenol, pH 8.0, 25C, recombinant His-tagged enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3e-06
-
P23457
substrate (R,S)-camphorquinone, mutant Y55F, pH 6.0, 25C
2e-05
-
P23457
substrate (R,S)-camphorquinone, mutant Y55S, pH 6.0, 25C
0.0005
-
-
medium alone
0.0006
-
P23457
substrate acenaphthenequinone, mutant Y55F, pH 6.0, 25C; substrate acenaphthenequinone, mutant Y55S, pH 6.0, 25C
0.0007
-
-
TNFalpha and LPS inhibit the beta-naphthoflavone induced increase in activity
0.0012
-
-
in presence of beta-naphthoflavone
0.0016
-
P23457
substrate 9,10-phenanthrenenquinone, mutant Y55S, pH 6.0, 25C
0.0018
-
P23457
substrate 9,10-phenanthrenenquinone, mutant Y55F, pH 6.0, 25C
0.0025
-
P23457
substrate (R,S)-camphorquinone, wild-type, pH 6.0, 25C
0.0038
-
P23457
substrate acenaphthenequinone, wild-type, pH 6.0, 25C
0.0044
-
P23457
substrate 9,10-phenanthrenenquinone, wild-type, pH 6.0, 25C
1.097
-
-
activity of purified cytochrome b558 reconstituted with neutrophil membrane phospholipids, activation with arachidonic acid
1.258
-
-
activity of purified cytochrome b558 reconstituted with neutrophil membrane phospholipids, activation with sodiumdodecylsulfate
1067
-
-
pH 7.5, 30C
additional information
-
-
90300 units/mg, 1 unit is defined as that amount of enzyme causing an absorption change of 0.001 units/min at the specific wavelength
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
7.5
-
-
6.5
-
-
-
7.4
-
-
assay at
7.5
-
-
assay at
8
-
-
assay at
9.1
-
-, Q8NKK9
calculation from nucleotide sequence
additional information
-
P23457
the pH dependency of 9,10-phenanthrenequinone reduction catalyzed by the wild-type enzyme is different to that observed for 3-ketosteroid reduction. The kcat value for 9,10-phenanthrenequinone reduction is pH-dependent with the maximal rate decreasing with increasing pH but reveals an ionizable group with a pKb of 8.90 that must be protonated for maximal activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
22
-
-
assay at room temperature
25
-
-
assay at
25
-
-
assay at
25
-
-
assay at
37
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
for genotyping
Manually annotated by BRENDA team
-
NQO1 activity is elevated (up to 20%) by ascorbigen treatment
Manually annotated by BRENDA team
-
soleus, gastrocnemius, and quadriceps muscles, primary culture of cells
Manually annotated by BRENDA team
additional information
-
no activity in endothelial cells, membrane-enriched
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
NADPH oxidase complex consists of membrane and cytosolic subunits
Manually annotated by BRENDA team
-
mitochondrial outer membrane
Manually annotated by BRENDA team
-
NADPH oxidase complex consists of membrane and cytosolic subunits. The gp91phox and p22phox are integral membrane proteins that form a heterodimeric flavocytochrome b558, the catalytic core of the enzyme
Manually annotated by BRENDA team
-
CBR4 is a mitochondrial matrix protein, its N-terminal signal is not cleaved
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30000
40000
-
ultracentrifugal analysis
42000
-
-
PAGE
60260
-
-
enzyme form DII, gel filtration
66070
-
-
enzyme form DI, gel filtration
84000
-
-, Q8NKK9
gel filtration
100000
-
-
gel filtration
additional information
-
-
enzyme displays three charge isomers with different relative mobilities
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 21000-22000, SDS-PAGE
?
Helicobacter hepaticus ATCC51449
-
x * 21000-22000, SDS-PAGE
-
dimer
-, Q8NKK9
2 * 42000, SDS-PAGE
octamer
-
8 * 5100, SDS-PAGE
tetramer
-
dimer of catalytically dependent dimers
tetramer
-
4 * 25000, SDS-PAGE
dimer
-
2 * 21000, SDS-PAGE
additional information
-
NADPH oxidase complex consists of membrane and cytosolic subunits. The gp91phox and p22phox are integral membrane proteins that form a heterodimeric flavocytochrome b558, the catalytic core of the enzyme
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
sitting drop vapour diffusion method. In addition to the oxidized and reduced enzyme form, the structures of the complexes with p-hydroxybenzaldehyde and p-nitrophenol, respectively are solved
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
cytochrome b558 from neutrophils
-
native enzyme partially by membrane preparation
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
cytosolic components p67-phox and p47-phox of the multicomponent NADPH oxidase
-
expression of p47-phox and p67-phox in Sf9 cells
-
overexpression of CBR4 in bovine endothelial cells reveals that the enzyme has a non-cleavable mitochondrial targeting signal
-
expression in Escherichia coli
-, Q8NKK9
gene arsh, expression as N-terminallyy His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
induction under different types of cellular stress, including ethanol and dimethylsulfoxide exposure and by reaching the stationary growth phase
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
P187S
-
Pro187Ser polymorphism in NQO1 has a limited role in the development of Tardive dyskinesia (a potentially irreversible side effect of antipsychotic medication treatment that occurs in approximately 25% of chronically treated schizophrenia patients)
D50N
P23457
less than 0.1% of wild-type activity
H117A
P23457
less than 0.1% of wild-type activity
K84M
P23457
complete loss of activity
K84R
P23457
complete loss of activity
Y55F
P23457
narrow substrate specificity, reduction of selected aromatic quinones and alpha-dicarbonyls. The activation energy for 9,10-phenanthrenequinone reduction is unchanged in Y55 mutants
Y55S
P23457
narrow substrate specificity, reduction of selected aromatic quinones and alpha-dicarbonyls. The activation energy for 9,10-phenanthrenequinone reduction is unchanged in Y55 mutants
Renatured/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
arsenic exposure is associated with an increased risk of urothelial carcinoma, inorganic arsenic, monomethylarsonic acid, and dimethylarsinic acid, are determined in urothelial carcinoma patients from arsenic-contaminated areas of southwestern Taiwan, no correlation of the arsenic methylation capability of the patients and C609T genotype of NQO1 is found
medicine
-
Pro187Ser polymorphism in NQO1 has a limited role in the development of Tardive dyskinesia (a potentially irreversible side effect of antipsychotic medication treatment that occurs in approximately 25% of chronically treated schizophrenia patients)