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Literature summary for 1.6.5.10 extracted from

  • Hayashi, M.; Ohzeki, H.; Shimada, H.; Unemoto, T.
    NADPH-specific quinone reductase is induced by 2-methylene-4-butyrolactone in Escherichia coli (1996), Biochim. Biophys. Acta, 1273, 165-170.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-methylene-4-butyrolactone 25fold induction Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
additional information insensitive to 4-chloromercuribenzoate at 100 microM, heavy metals such as Cu2+, Co2+, Cd2+ and Mn2+ at 10 microM each, and chelating agents such as EDTA, o-phenanthroline and diethyldithiocarbamate at 100 microM each Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
menadione pH 7.5, 30°C Escherichia coli
0.0105
-
NADPH pH 7.5, 30°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Escherichia coli
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
enzyme displays three charge isomers with different relative mobilities Escherichia coli
21000
-
2 * 21000, SDS-PAGE Escherichia coli
42000
-
PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1067
-
pH 7.5, 30°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-benzoquinone + NADPH + H+ about 81% of the activity with menadione Escherichia coli 1,4-benzoquinol + NADP+
-
?
1,4-naphthoquinone + NADPH + H+ about 85% of the activity with menadione Escherichia coli 1,4-naphthoquinol + NADP+
-
?
2 ferricyanide + NADPH about 90% of the activity with menadione Escherichia coli 2 ferrocyanide + NADP+ + H+
-
?
dichlorophenolindophenol + NADPH + H+ about 15% of the activity with menadione Escherichia coli ? + NADP+
-
?
menadione + NADPH + H+ reduction follows a two-electron-pathway Escherichia coli menadiol + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 21000, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FAD enzyme displays flavin absorption spectrum with peaks at 376 and 456 nm and a shoulder at around 484 nm. Protein caontains FAD Escherichia coli
additional information no cofactor: NADH, FMN Escherichia coli
NADPH specific Escherichia coli