Reference on EC 1.1.1.50 - 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) and Organism(s) Comamonas testosteroni and UniProt Accession P80702
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Skalhegg, B.A.
On the 3alpha-hydroxysteroid dehydrogenase from Pseudomonas testosteroni. Purification and properties
Eur. J. Biochem.
46
117-125
1974
Comamonas testosteroni
Skalhegg, B.A.
3alpha-Hydroxysteroid dehydrogenase from Pseudomonas testosteroni: kinetic properties with NAD and its thionicotinamide analogue
Eur. J. Biochem.
50
603-609
1975
Comamonas testosteroni
Jarabak, J.; Talalay, P.
Stereospecificity of hydrogen transfer by pyridine nucleotide-linked hydroxysteroid dehydrogenase
J. Biol. Chem.
235
2147-2151
1960
Comamonas testosteroni
Squire, P.G.; Dehlin, S.; Porath, J.
Physical and chemical characterization of hydroxysteroid dehydrogenases from Pseudomonas testosteroni
Biochim. Biophys. Acta
89
409-421
1964
Comamonas testosteroni
Maser, E.; Mobus, E.; Xiong, G.
Functional expression, purification, and characterization of 3alpha-hydroxysteroid dehydrogenase/Carbonyl reductase from Comamonas testosteroni
Biochem. Biophys. Res. Commun.
272
622-628
2000
Comamonas testosteroni
Yee, D.J.; Balsanek, V.; Sames, D.
New tools for molecular imaging of redox metabolism: development of a fluorogenic probe for 3a-hydroxysteroid dehydrogenases
J. Am. Chem. Soc.
126
2282-2283
2004
Comamonas testosteroni, Homo sapiens, Rattus norvegicus
Hwang, C.; Chang, Y.; Hsu, C.; Hsu, H.; Li, C.; Pon, H.
Mechanistic roles of Ser-114, Tyr-155, and Lys-159 in 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni
J. Biol. Chem.
280
3522-3528
2005
Comamonas testosteroni
Okochi, M.; Nakagawa, I.; Kobayashi, T.; Hayashi, S.; Furusaki, S.; Honda, H.
Enhanced activity of 3alpha-hydroxysteroid dehydrogenase by addition of the co-solvent 1-butyl-3-methylimidazolium (l)-lactate in aqueous phase of biphasic systems for reductive production of steroids
J. Biotechnol.
128
376-382
2006
Comamonas testosteroni
Chang, Y.H.; Chuang, L.Y.; Hwang, C.C.
Mechanism of proton transfer in the 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni
J. Biol. Chem.
282
34306-34314
2007
Comamonas testosteroni (P80702)
Hoffmann, F.; Sotriffer, C.; Evers, A.; Xiong, G.; Maser, E.
Understanding oligomerization in 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni: an in silico approach and evidence for an active protein
J. Biotechnol.
129
131-139
2007
Comamonas testosteroni (P80702)
Hwang, C.C.; Hsu, C.N.; Huang, T.J.; Chiou, S.J.; Hong, Y.R.
Interactions across the interface contribute the stability of homodimeric 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase
Arch. Biochem. Biophys.
490
36-41
2009
Comamonas testosteroni (P80702)
Chang, Y.H.; Huang, T.J.; Chuang, L.Y.; Hwang, C.C.
Role of S114 in the NADH-induced conformational change and catalysis of 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni
Biochim. Biophys. Acta
1794
1459-1466
2009
Comamonas testosteroni (P80702)
Xiong, G.; Draus, E.; Luo, Y.; Maser, E.
3alpha-Hydroxysteroid dehydrogenase/carbonyl reductase as a tool for isolation and characterization of a new marine steroid degrading bacterial strain
Chem. Biol. Interact.
178
206-210
2009
Comamonas testosteroni
Xiong, G.; Luo, Y.; Jin, S.; Maser, E.
Cis- and trans-regulatory elements of 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase as biosensor system for steroid determination in the environment
Chem. Biol. Interact.
178
215-220
2009
Comamonas testosteroni, Comamonas testosteroni (P80702)
Chang, Y.H.; Wang, C.Z.; Chiu, C.C.; Chuang, L.Y.; Hwang, C.C.
Contributions of active site residues to cofactor binding and catalysis of 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase
Biochim. Biophys. Acta
1804
235-241
2010
Comamonas testosteroni (P80702)
Mundaca, R.A.; Moreno-Guzman, M.; Eguilaz, M.; Yanez-Sedeno, P.; Pingarron, J.M.
Enzyme biosensor for androsterone based on 3alpha-hydroxysteroid dehydrogenase immobilized onto a carbon nanotubes/ionic liquid/NAD+ composite electrode
Talanta
99
697-702
2012
Comamonas testosteroni (P80702)
Yang, H.; Fang, Y.; Wang, Z.; Zhang, L.
One step affinity recovery of 3alpha-hydroxysteroid dehydrogenase from cloned Escherichia coli
J. Chromatogr. B
991
79-84
2015
Comamonas testosteroni (P80702), Comamonas testosteroni ATCC 11996 (P80702)
Hwang, C.C.; Chang, Y.H.; Lee, H.J.; Wang, T.P.; Su, Y.M.; Chen, H.W.; Liang, P.H.
The catalytic roles of P185 and T188 and substrate-binding loop flexibility in 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni
PLoS ONE
8
e63594
2013
Comamonas testosteroni (P80702)
Hwang, C.C.; Chang, P.R.; Wang, T.P.
Contribution of remote substrate binding energy to the enzymatic rate acceleration for 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase
Chem. Biol. Interact.
276
133-140
2017
Comamonas testosteroni (P80702)