Information on EC 1.1.1.100 - 3-oxoacyl-[acyl-carrier-protein] reductase

1.1.1.100

-

3-oxoacyl-[acyl-carrier-protein] reductase

GO:0004316

a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

belongs to family of short-chain alcohol dehydrogenases (SDR) with catalytic triad Ser154, Tyr167 and Lys171, catalytic mechanism

Brassica napus

-

285683

a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

ordered bi bi kinetic mechanism, [acyl-carrier protein] recognition mechanism

Plasmodium falciparum

Q86RB1

667520

a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

reaction mechanism, random bi bi kinetic mechanism

Streptococcus pneumoniae

-

667646

a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

chemical and kinetic mechanism

Mycobacterium tuberculosis

-

667708

a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

cofactor and substrate binding kinetics, allosteric regulation mechanism, overview

Plasmodium falciparum

-

668574

a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

positive cooperativity in binding of NADPH to enzyme, no cooperativity in binding of acetoacyl-CoA. Pre-existing equilibrium of two forms of free MabA in solution followed by a bimolecular association process

Mycobacterium tuberculosis

-

684702

a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

-

-

-

-

oxidation

-

-

-

-

redox reaction

-

-

-

-

redox reaction

Pseudomonas putida

A5HIF6, -

-

693287

reduction

-

-

-

-

8-amino-7-oxononanoate biosynthesis I

-

PWY-6519

cis-dodecenoyl biosynthesis

-

PWY0-862

cis-vaccenate biosynthesis

-

PWY-5973

docosahexanoate biosynthesis I

-

PWY-7053

docosahexanoate biosynthesis II

-

PWY-6951

fatty acid elongation -- saturated

-

FASYN-ELONG-PWY

mycolate biosynthesis

-

PWYG-321

octanoyl-ACP biosynthesis (mitochondria, yeast)

-

PWY-7388

palmitate biosynthesis I (animals and fungi)

-

PWY-5994

palmitate biosynthesis II (bacteria and plants)

-

PWY-5971

palmitoleate biosynthesis I

-

PWY-6282

petroselinate biosynthesis

-

PWY-5367

stearate biosynthesis II (bacteria and plants)

-

PWY-5989

stearate biosynthesis III (fungi)

-

PWY3O-355

superpathway of mycolate biosynthesis

-

PWY-6113

Fatty acid biosynthesis

00061

-

Biotin metabolism

00780

-

Biosynthesis of unsaturated fatty acids

01040

-

Metabolic pathways

01100

-

(3R)-3-hydroxyacyl-[acyl-carrier protein]:NADP+ oxidoreductase

Exhibits a marked preference for acyl-carrier-protein derivatives over CoA derivatives as substrates.

3-ketoacyl acyl carrier protein reductase

-

-

-

-

3-ketoacyl-(acyl-carrier-protein) reductase

Rickettsia prowazekii

P50941

-

723843

3-ketoacyl-ACP reductase

Pseudomonas putida

A5HIF6

-

693287

3-ketoacyl-ACP(CoA) reductase

Mycobacterium tuberculosis

-

-

657270

3-ketoacyl-acyl carrier protein reductase

-

-

-

-

3-ketoacyl-acyl carrier protein reductase

Escherichia coli

-

-

667257

3-ketoacyl-acyl carrier protein reductase

Pseudomonas putida

A5HIF6

-

693287

3-ketoacyl-acyl carrier protein reductase

Pseudomonas sp.

Q4AE87

-

667257

3-ketoacyl-[acyl-carrier-protein] reductase

Synechococcus elongatus PCC 7942

Q31QF3

-

701783

3-oxoacyl-(acyl carrier protein) reductase

Bacillus anthracis

Q81JG6

-

706640

3-oxoacyl-ACP reductase

Plasmodium falciparum

Q86RB1

-

667520

3-oxoacyl-ACP reductase

Saccharomyces cerevisiae

-

-

704725

3-oxoacyl-AcpM reductase

Mycobacterium tuberculosis

O53665, P0A5Y4

-

705747

3-oxoacyl-thioester reductase

Caenorhabditis elegans

-

-

704725

3-oxoacyl-thioester reductase

Mycobacterium tuberculosis, Saccharomyces cerevisiae

-

-

705747

3-oxoacyl-[ACP]reductase

-

-

-

-

ACP reductase

Mycobacterium tuberculosis

-

-

667708

beta-ketoacyl acyl carrier protein (ACP) reductase

-

-

-

-

beta-ketoacyl acyl carrier protein reductase

Plasmodium falciparum

-

-

668574, 703630

beta-ketoacyl acyl carrier protein reductase

Pseudomonas aeruginosa

-

-

656723

beta-ketoacyl reductase

-

-

-

-

beta-ketoacyl reductase

Arabidopsis thaliana

-

-

700827

beta-ketoacyl reductase

Homo sapiens

-

-

669416

beta-ketoacyl reductase

Pseudomonas aeruginosa

-

-

669470

beta-ketoacyl thioester reductase

-

-

-

-

beta-ketoacyl-ACP reductase

-

-

-

-

beta-ketoacyl-ACP reductase

Acinetobacter calcoaceticus

-

-

706142

beta-ketoacyl-ACP reductase

Acinetobacter calcoaceticus 25001

-

-

-

beta-ketoacyl-ACP reductase

Enterobacter aerogenes

-

-

706142

beta-ketoacyl-ACP reductase

Enterobacter aerogenes 45102

-

-

-

beta-ketoacyl-ACP reductase

Enterobacter cloacae

-

-

706142

beta-ketoacyl-ACP reductase

Enterobacter cloacae 45301

-

-

-

beta-ketoacyl-ACP reductase

Enterococcus sp.

-

-

706142

beta-ketoacyl-ACP reductase

Escherichia coli

-

-

669013, 703163, 704337, 706142

beta-ketoacyl-ACP reductase

Haloarcula hispanica

-

-

701808

beta-ketoacyl-ACP reductase

Haloarcula hispanica AS2049, Haloarcula hispanica CGMCC1.2049

-

-

-

beta-ketoacyl-ACP reductase

Haloferax volcanii

-

-

701808

beta-ketoacyl-ACP reductase

Klebsiella pneumoniae

-

-

706142

beta-ketoacyl-ACP reductase

Mycobacterium tuberculosis

-

-

724676

beta-ketoacyl-ACP reductase

Plasmodium falciparum

-

-

670774, 706639

beta-ketoacyl-ACP reductase

Plasmodium falciparum K1

-

-

689435

beta-ketoacyl-ACP reductase

Proteus vulgaris

-

-

706142

beta-ketoacyl-ACP reductase

Pseudomonas aeruginosa

-

-

704843, 706142

beta-ketoacyl-ACP reductase

Pseudomonas aeruginosa ATCC27853

-

-

-

beta-ketoacyl-ACP reductase

Salmonella enterica subsp. enterica serovar Typhi

-

-

706142

beta-ketoacyl-ACP reductase

Salmonella enterica subsp. enterica serovar Typhi H901

-

-

-

beta-ketoacyl-ACP reductase

Salmonella enterica subsp. enterica serovar Typhimurium, Serratia marcescens

-

-

706142

beta-ketoacyl-ACP reductase

Serratia marcescens 41002

-

-

-

beta-ketoacyl-ACP reductase

Shigella dysenteriae, Shigella flexneri, Shigella sonnei

-

-

706142

beta-ketoacyl-ACP reductase

Shigella sonnei 51592

-

-

-

beta-ketoacyl-ACP reductase

Staphylococcus aureus

-

-

704843, 706142

beta-ketoacyl-ACP reductase

Staphylococcus aureus ATCC25923

-

-

-

beta-ketoacyl-ACP reductase

Staphylococcus epidermidis

-

-

706142

beta-ketoacyl-ACP reductase

Streptococcus pneumoniae

-

-

667646, 704843

beta-ketoacyl-ACP reductase

Streptococcus sp., synthetic construct

-

-

704843

beta-ketoacyl-acyl carrier protein reductase

-

-

-

-

beta-ketoacyl-acyl carrier protein reductase

Escherichia coli

-

-

655871

beta-ketoacyl-acyl carrier protein reductase

Mycobacterium tuberculosis

-

-

667708

beta-ketoacyl-acyl carrier protein reductase

Salmonella enterica subsp. enterica serovar Typhimurium

-

-

655871

beta-ketoacyl-[acyl carrier protein] reductase

Escherichia coli

-

-

703163

beta-ketoacyl-[acyl-carrier protein] (ACP) reductase

-

-

-

-

BKR

Brassica napus

-

-

285682, 285683

BKR

Escherichia coli

-

-

285681, 285683

BKR

Homo sapiens

-

-

669416

FabG

Acinetobacter calcoaceticus

-

-

706142

FabG

Acinetobacter calcoaceticus 25001

-

-

-

FabG

Enterobacter aerogenes

-

-

706142

FabG

Enterobacter aerogenes 45102

-

-

-

FabG

Enterobacter cloacae

-

-

706142

FabG

Enterobacter cloacae 45301

-

-

-

FabG

Enterococcus sp.

-

-

706142

FabG

Escherichia coli

-

-

655871, 667257, 669013, 703163, 704337, 706142

FabG

Klebsiella pneumoniae

-

-

706142

FabG

Plasmodium falciparum

-

-

654432, 668574, 670774, 703630, 706639

FabG

Plasmodium falciparum

Q86RB1

-

667520

FabG

Plasmodium falciparum K1

-

-

689435

FabG

Proteus vulgaris

-

-

706142

FabG

Pseudomonas aeruginosa

-

-

656723, 704843, 706142

FabG

Pseudomonas aeruginosa ATCC27853

-

-

-

FabG

Pseudomonas putida

A5HIF6

-

693287

FabG

Pseudomonas sp.

Q4AE87

-

667257

FabG

Rickettsia prowazekii

P50941

gene name

723843

FabG

Salmonella enterica subsp. enterica serovar Typhi

-

-

706142

FabG

Salmonella enterica subsp. enterica serovar Typhi H901

-

-

-

FabG

Salmonella enterica subsp. enterica serovar Typhimurium

-

-

655871, 706142

FabG

Serratia marcescens

-

-

706142

FabG

Serratia marcescens 41002

-

-

-

FabG

Shigella dysenteriae, Shigella flexneri, Shigella sonnei

-

-

706142

FabG

Shigella sonnei 51592

-

-

-

FabG

Staphylococcus aureus

-

-

704843, 706142

FabG

Staphylococcus aureus ATCC25923

-

-

-

FabG

Staphylococcus epidermidis

-

-

706142

FabG

Streptococcus pneumoniae, Streptococcus sp., synthetic construct

-

-

704843

FabG1

Haloarcula hispanica

-

-

701808

FabG1

Haloarcula hispanica AS2049, Haloarcula hispanica CGMCC1.2049

-

-

-

FabG1

Haloferax volcanii

-

-

701808

FabG1

Lactococcus lactis

-

-

654725

FabG1

Mycobacterium tuberculosis

P0A5Y4

-

705747

fabG4

Mycobacterium tuberculosis

-, O53665

-

705747

fabG4

Saccharomyces cerevisiae

-

-

705747

KACPR

Streptococcus pneumoniae

-

-

667646

KCR1

Arabidopsis thaliana

-

-

700827

KCR2

Arabidopsis thaliana

-

-

700827

MabA

Mycobacterium tuberculosis

-

-

667708, 724676

NADPH-specific 3-oxoacyl-[acylcarrier protein]reductase

-

-

-

-

OAR

Plasmodium falciparum

Q86RB1

-

667520

Oar1p

Saccharomyces cerevisiae

-

-

704725

PHA-specific acetoacetyl-CoA reductase

Haloarcula hispanica

-

-

701808

PHA-specific acetoacetyl-CoA reductase

Haloarcula hispanica AS2049, Haloarcula hispanica CGMCC1.2049

-

-

-

PHA-specific acetoacetyl-CoA reductase

Haloferax volcanii

-

-

701808

PhaB

Haloarcula hispanica

-

-

701808

PhaB

Haloarcula hispanica AS2049, Haloarcula hispanica CGMCC1.2049

-

-

-

PhaB

Haloferax volcanii

-

-

701808

polyhydroxyalkanoate-specific acetoacetyl coenzyme A reductase

Haloarcula hispanica

-

-

701808

polyhydroxyalkanoate-specific acetoacetyl coenzyme A reductase

Haloarcula hispanica AS2049, Haloarcula hispanica CGMCC1.2049

-

-

-

polyhydroxyalkanoate-specific acetoacetyl coenzyme A reductase

Haloferax volcanii

-

-

701808

reductase, 3-oxoacyl-[acyl carrier protein]

-

-

-

-

RhlG

Pseudomonas aeruginosa

-

-

669470

37250-34-3

-

Acinetobacter calcoaceticus

strain 25001

706142

-

-

Acinetobacter calcoaceticus 25001

strain 25001

706142

-

-

Aquifex aeolicus VF5

isoform FabG

684165

-

-

Arabidopsis thaliana

-

700827

-

-

Bacillus anthracis

-

689973, 706640

Q81JG6

SwissProt

Bacillus subtilis

-

285680

-

-

Brassica juncea

rapeseed

285675

-

-

Brassica napus

-

285682, 285683

-

-

Brassica napus

-

689500

Q949M3

Uniprot

Brassica napus

oilseed

285670, 285673

-

-

Caenorhabditis elegans

-

704725

-

-

Carthamus tinctorius

safflower

285669, 285675

-

-

Cuphea lanceolata

-

285679

-

-

Cuphea lutea

-

285675

-

-

Enterobacter aerogenes

strain 45102

706142

-

-

Enterobacter aerogenes 45102

strain 45102

706142

-

-

Enterobacter cloacae

strain 45301

706142

-

-

Enterobacter cloacae 45301

strain 45301

706142

-

-

Enterococcus sp.

strains ATCC29212 and 775

706142

-

-

Escherichia coli

-

285668, 285671, 285672, 285681, 285683, 655871, 685704, 685775, 704337

-

-

Escherichia coli

-

703163

P0AEK2

Uniprot

Escherichia coli

; strains ATCC 25922 and 26

706142

-

-

Escherichia coli

gene fabG

667257, 669013

-

-

Euglena gracilis

-

285676

-

-

Haloarcula hispanica

strain AS2049

701808

-

-

Haloarcula hispanica AS2049

strain AS2049

701808

-

-

Haloferax volcanii

-

701808

-

-

Homo sapiens

-

669416

-

-

Klebsiella pneumoniae

strains ATCC700603 and 14

706142

-

-

Lactococcus lactis

ssp. lactis IL 1403

654725

-

-

Mycobacterium tuberculosis

-

657270, 705747, 724676

-

-

Mycobacterium tuberculosis

-

705747

O53665, P0A5Y4

UniProt

Mycobacterium tuberculosis

gene mabA

667708

-

-

Mycobacterium tuberculosis

isoform MabA

684702

-

-

Persea americana

-

285674, 285675, 285678

-

-

Persea americana

avocado

285670

-

-

Pisum sativum

-

285675

-

-

Plasmodium falciparum

-

654432

Q965D6

Uniprot

Plasmodium falciparum

-

670774, 687216, 703630, 706639

-

-

Plasmodium falciparum

gene fabG

667520

Q86RB1

SwissProt

Plasmodium falciparum

gene fabG, no isozymes

668574

-

-

Plasmodium falciparum K1

-

689435

-

-

Proteus vulgaris

-

706142

-

-

Pseudomonas aeruginosa

-

656723, 685704

-

-

Pseudomonas aeruginosa

ATCC27853

704843

-

-

Pseudomonas aeruginosa

gene rhlG

669470

-

-

Pseudomonas aeruginosa

strains ATCC27853 and 17

706142

-

-

Pseudomonas aeruginosa ATCC27853

ATCC27853

704843

-

-

Pseudomonas putida

-

693287

A5HIF6

UniProt

Pseudomonas sp.

strain 61-3, non-pathogenic strain from soil, gene fabG

667257

Q4AE87

SwissProt

Rickettsia prowazekii

-

723843

P50941

UniProt

Saccharomyces cerevisiae

-

704725, 705747

-

-

Salmonella enterica subsp. enterica serovar Typhi

strain H901

706142

-

-

Salmonella enterica subsp. enterica serovar Typhi H901

strain H901

706142

-

-

Salmonella enterica subsp. enterica serovar Typhimurium

-

706142

-

-

Salmonella enterica subsp. enterica serovar Typhimurium

serovar typhimurium

655871

-

-

Serratia marcescens

strain 41002

706142

-

-

Serratia marcescens 41002

strain 41002

706142

-

-

Shigella dysenteriae

-

706142

-

-

Shigella flexneri

-

706142

-

-

Shigella sonnei

strain 51592

706142

-

-

Shigella sonnei 51592

strain 51592

706142

-

-

Spinacia oleracea

-

285668, 285675, 285677

-

-

Staphylococcus aureus

ATCC25923

704843

-

-

Staphylococcus aureus

strains ATCC29213, 15 and 05-3

706142

-

-

Staphylococcus aureus ATCC25923

ATCC25923

704843

-

-

Staphylococcus epidermidis

-

685704

-

-

Staphylococcus epidermidis

strains ATCC12228, 04-5 and 05-1

706142

-

-

Streptococcus pneumoniae

-

704843

-

-

Streptococcus pneumoniae

gene fabG

667646

-

-

Streptococcus sp.

alpha-haemolytic strain 32213-7 and beta-haemolytic strain 32210-20

704843

-

-

Synechococcus elongatus PCC 7942

-

701783

Q31QF3

UniProt

synthetic construct

-

704843

-

-

malfunction

Arabidopsis thaliana

-

loss of KCR1 function results in embryo lethality, which cannot be rescued by KCR2 expression using the KCR1 promoter. Disruption of the KCR2 gene has no obvious phenotypic effect

700827

malfunction

Haloarcula hispanica

-

DELTAfabG1 cells lose the ability to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate). When the DELTAfabG1 strain is transformed with the fabG1 expression plasmid pWLG1, the capability of polyhydroxyalkanoate accumulation is restored. When the fabG1 gene is disrupted or knocked out, the NADPH-dependent activity of acetoacetyl-CoA reductase in the mutant, the DELTAfabG1 or fabG1-1 strain, is greatly decreased

701808

malfunction

Haloferax volcanii

-

polyhydroxyalkanoate-defective haloarchaeon

701808

malfunction

Haloarcula hispanica AS2049, Haloarcula hispanica CGMCC1.2049

-

DELTAfabG1 cells lose the ability to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate). When the DELTAfabG1 strain is transformed with the fabG1 expression plasmid pWLG1, the capability of polyhydroxyalkanoate accumulation is restored. When the fabG1 gene is disrupted or knocked out, the NADPH-dependent activity of acetoacetyl-CoA reductase in the mutant, the DELTAfabG1 or fabG1-1 strain, is greatly decreased

-

physiological function

Arabidopsis thaliana

-

only KCR1 is a functional KCR isoform involved in microsomal fatty acid elongation

700827

physiological function

Haloarcula hispanica

-

FabG1 has the function of supplying precursors for polyhydroxyalkanoate biosynthesis

701808

physiological function

Haloarcula hispanica AS2049, Haloarcula hispanica CGMCC1.2049

-

FabG1 has the function of supplying precursors for polyhydroxyalkanoate biosynthesis

-

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

3-oxoacyl-[acyl-carrier protein] + NADPH + H+

Pseudomonas putida

A5HIF6, -

beta-oxidation pathway

693287

-

-

?

1,1,1-trifluoroacetone + NADPH

? + NADP+

Synechococcus elongatus PCC 7942

Q31QF3

792% relative activity compared to acetophenone

701783

-

-

?

2',3',4',5',6'-pentafluoroacetophenone + NADPH

? + NADP+

Synechococcus elongatus PCC 7942

Q31QF3

3023% relative activity compared to acetophenone

701783

-

-

?

2-octanone + NADPH

? + NADP+

Synechococcus elongatus PCC 7942

Q31QF3

15% relative activity compared to acetophenone

701783

-

-

?

3'-fluoroacetophenone + NADPH

? + NADP+

Synechococcus elongatus PCC 7942

Q31QF3

100% relative activity compared to acetophenone

701783

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Escherichia coli

-

-

285671, 285683

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Escherichia coli

-

-

285683

-

285683

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Escherichia coli

-

-

285668

-

285668

r

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Escherichia coli

-

-

285681

-

285681

r

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Euglena gracilis

-

-

285676

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Spinacia oleracea

-

-

285675, 285677

-

-

-

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Spinacia oleracea

-

-

285668

-

285668

r

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Pisum sativum

-

-

285675

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Brassica napus

-

-

285670, 285673

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Brassica napus

-

-

285682

-

285682

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Plasmodium falciparum

-

-

706639

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Persea americana

-

-

285670, 285674, 285675, 285678

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Brassica juncea

-

-

285675

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Carthamus tinctorius

-

-

285669, 285675

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Cuphea lutea

-

-

285675

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Brassica napus

-

belongs to family of short-chain alcohol dehydrogenases (SDR) with catalytic triade Ser154, Tyr167 and Lys171, catalytic mechanism

285683

-

285683

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Escherichia coli

-

pH 6.0-7.0, equilibrium almost completely favors formation of the beta-hydroxyacyl ACP derivatives

285672

product is the D-(-)-stereoisomer

285672

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Brassica napus

-

enzyme is part of the dissociable fatty acid synthase type II multienzyme complex for de novo synthesis of fatty acids from acetyl-CoA and malonyl-CoA

285683

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Escherichia coli

-

first reduction step of fatty acid synthase

285671

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Brassica napus, Persea americana

-

first reduction step of fatty acid synthase

285670

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Spinacia oleracea

-

enzyme in fatty acid synthesis

285677

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Brassica napus

-

enzyme in fatty acid synthesis

285683

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Carthamus tinctorius

-

enzyme in fatty acid synthesis

285669

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Euglena gracilis Z

-

-

285676

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

3-hydroxyacyl-[acyl-carrier protein] + NADP+

Mycobacterium tuberculosis

-

-

667708

-

-

r

3-oxoacyl-[acyl-carrier protein] + NADPH

3-hydroxyacyl-[acyl-carrier protein] + NADP+

Plasmodium falciparum

-

-, cooperative transitions in the enzyme due to cofactor and [acyl-carrier-protein] binding

668574

-

-

r

4'-chloroacetophenone + NADPH

? + NADP+

Synechococcus elongatus PCC 7942

Q31QF3

115% relative activity compared to acetophenone

701783

-

-

?

4'-fluoroacetophenone + NADPH

? + NADP+

Synechococcus elongatus PCC 7942

Q31QF3

62% relative activity compared to acetophenone

701783

-

-

?

acetoacetyl-CoA + NADPH

D-beta-hydroxybutyryl-CoA + NADP+

Salmonella enterica subsp. enterica serovar Typhimurium

-

-

655871

-

-

?

acetoacetyl-CoA + NADPH

D-beta-hydroxybutyryl-CoA + NADP+

Escherichia coli

-

-

285668, 285683, 655871

-

-

?

acetoacetyl-CoA + NADPH

D-beta-hydroxybutyryl-CoA + NADP+

Escherichia coli

P0AEK2

-

703163

-

-

?

acetoacetyl-CoA + NADPH

D-beta-hydroxybutyryl-CoA + NADP+

Escherichia coli

-

-

285672

-

285672

r

acetoacetyl-CoA + NADPH

D-beta-hydroxybutyryl-CoA + NADP+

Spinacia oleracea

-

-

285668

-

-

?

acetoacetyl-CoA + NADPH

D-beta-hydroxybutyryl-CoA + NADP+

Brassica napus

-

-

285673

-

-

-

acetoacetyl-CoA + NADPH

D-beta-hydroxybutyryl-CoA + NADP+

Brassica napus

-

-

285670, 285683

-

-

?

acetoacetyl-CoA + NADPH

D-beta-hydroxybutyryl-CoA + NADP+

Plasmodium falciparum

Q965D6

-

654432

-

-

?

acetoacetyl-CoA + NADPH

D-beta-hydroxybutyryl-CoA + NADP+

Persea americana

-

-

285674

-

-

-

acetoacetyl-CoA + NADPH

D-beta-hydroxybutyryl-CoA + NADP+

Persea americana

-

-

285670

-

-

?

acetoacetyl-CoA + NADPH

D-beta-hydroxybutyryl-CoA + NADP+

Cuphea lanceolata

-

-

285679

-

-

?

acetoacetyl-CoA + NADPH

D-3-hydroxybutyryl-CoA + NADP+

Mycobacterium tuberculosis

-

-

724676

-

-

?

acetoacetyl-CoA + NADPH

3-hydroxybutyryl-CoA + NADP+

Escherichia coli

-

-

667257, 669013

-

-

?

acetoacetyl-CoA + NADPH

3-hydroxybutyryl-CoA + NADP+

Homo sapiens

-

-

669416

-

-

?

acetoacetyl-CoA + NADPH

3-hydroxybutyryl-CoA + NADP+

Streptococcus pneumoniae

-

-

667646

-

-

?

acetoacetyl-CoA + NADPH

3-hydroxybutyryl-CoA + NADP+

Mycobacterium tuberculosis

-

-

667708

-

-

r

acetoacetyl-CoA + NADPH

3-hydroxybutyryl-CoA + NADP+

Plasmodium falciparum

-

-

670774, 706639

-

-

?

acetoacetyl-CoA + NADPH

3-hydroxybutyryl-CoA + NADP+

Plasmodium falciparum

-

-

668574

-

-

r

acetoacetyl-CoA + NADPH

3-hydroxybutyryl-CoA + NADP+

Pseudomonas sp.

-, Q4AE87

-

667257

-

-

?

acetoacetyl-CoA + NADPH

3-hydroxybutyryl-CoA + NADP+

Plasmodium falciparum

Q86RB1

-

667520

-

-

?

acetoacetyl-CoA + NADPH

3-hydroxybutyryl-CoA + NADP+

Plasmodium falciparum K1

-

-

689435

-

-

?

acetoacetyl-CoA + NADPH

? + NADP+

Plasmodium falciparum

-

wild-type shows less activity with acetoacetyl-CoA than with acetoacetyl-[acyl-carrier protein]

703630

-

-

?

acetoacetyl-N-cysteamine + NADPH

D-3-hydroxybutyryl-N-cysteamine + NADP+

Escherichia coli

-

-

285683

-

-

?

acetoacetyl-N-cysteamine + NADPH

D-3-hydroxybutyryl-N-cysteamine + NADP+

Brassica napus

-

-

285670, 285673, 285683

-

-

?

acetoacetyl-N-cysteamine + NADPH

D-3-hydroxybutyryl-N-cysteamine + NADP+

Persea americana

-

-

285670, 285674

-

-

?

acetoacetyl-[acyl-carrier protein] + NADPH

D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+

Escherichia coli

-

-

285683

-

-

?

acetoacetyl-[acyl-carrier protein] + NADPH

D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+

Escherichia coli

-

-

285668

-

285668

r

acetoacetyl-[acyl-carrier protein] + NADPH

D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+

Escherichia coli

-

-

285672

-

285672

r

acetoacetyl-[acyl-carrier protein] + NADPH

D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+

Spinacia oleracea

-

-

285677

-

-

-

acetoacetyl-[acyl-carrier protein] + NADPH

D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+

Spinacia oleracea

-

-

285668

-

285668

r

acetoacetyl-[acyl-carrier protein] + NADPH

D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+

Brassica napus

-

-

285673

-

-

-

acetoacetyl-[acyl-carrier protein] + NADPH

D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+

Brassica napus

-

-

285683

-

-

?

acetoacetyl-[acyl-carrier protein] + NADPH

D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+

Persea americana

-

-

285674

-

-

-

acetoacetyl-[acyl-carrier protein] + NADPH

D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+

Carthamus tinctorius

-

-

285669

-

-

-

acetoacetyl-[acyl-carrier protein] + NADPH

D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+

Brassica napus, Persea americana

-

much greater specificity for acetoacetyl-ACP than for either of the model substrates acetoacetyl-CoA or acetoacetyl-N-acetylcysteamine

285670

-

-

?

acetoacetyl-[acyl-carrier protein] + NADPH

D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+

Plasmodium falciparum

-

Arg187 and Arg230 are critical residues for the FabG-acyl-carrier protein interactions, significance of the positively charged/hydrophobic patch located adjacent to the active site cavities of FabG for interactions with acyl carrier protein

703630

-

-

?

acetophenone + NADPH

? + NADP+

Synechococcus elongatus PCC 7942

Q31QF3

100% relative activity

701783

-

-

?

alpha-chloroacetophenone + NADPH

? + NADP+

Synechococcus elongatus PCC 7942

Q31QF3

1885% relative activity compared to acetophenone

701783

-

-

?

beta-ketoacyl-[acyl-carrier protein] + NADPH

beta-hydroxyacyl-[acyl-carrier protein] + NADP+

Salmonella enterica subsp. enterica serovar Typhimurium, Escherichia coli

-

first reductive step in the elongation cycle of fatty acid biosynthesis

655871

-

-

?

beta-ketobutyryl-CoA + NADPH

beta-hydroxybutyryl-CoA + NADP+

Lactococcus lactis

-

complementation of Escherichia coli fabG mutant with Lactobacillus lactis fabG genes shows that fabG1 encodes a functional beta-ketoacyl ACP reductase and fabG2 encodes an enzyme that has activity with the model substrate beta-ketobutyryl-CoA, but lacks the ability to support fatty acid synthesis both in vivo and in vitro

654725

-

-

-

crotonyl-CoA + NADPH

?

Homo sapiens

-

-

669416

-

-

?

dec-2-enoyl-CoA + NADPH

3-hydroxydecanoyl-CoA + NADP+

Escherichia coli, Pseudomonas sp.

-

-

667257

-

-

?

dec-2-enoyl-CoA + NADPH

3-hydroxydecanoyl-CoA + NADP+

Pseudomonas sp.

Q4AE87

-

667257

-

-

?

dodec-2-enoyl-CoA + NADPH

3-hydroxydodecanoyl-CoA + NADP+

Escherichia coli

-

-

667257

-

-

?

dodec-2-enoyl-CoA + NADPH

3-hydroxydodecanoyl-CoA + NADP+

Homo sapiens

-

-

669416

-

-

?

dodec-2-enoyl-CoA + NADPH

3-hydroxydodecanoyl-CoA + NADP+

Pseudomonas sp.

-, Q4AE87

-

667257

-

-

?

ethyl 4-chloroacetoacetate + NADPH

? + NADP+

Synechococcus elongatus PCC 7942

Q31QF3

29454% relative activity compared to acetophenone

701783

-

-

?

ethyl acetoacetate + NADPH

? + NADP+

Escherichia coli

-

-

706142

-

-

?

ethyl acetoacetate + NADPH

ethyl 3-hydroxybutyrate + NADP+

Escherichia coli

-

-

704337

-

-

?

ethyl acetoacetate + NADPH

ethyl 3-hydroxybutyrate + NADP+

synthetic construct

-

-

704843

-

-

?

ethylbenzoylacetate + NADPH

? + NADP+

Synechococcus elongatus PCC 7942

Q31QF3

892% relative activity compared to acetophenone

701783

-

-

?

hex-2-enoyl-CoA + NADPH

3-hydroxyhexanoyl-CoA + NADP+

Escherichia coli, Pseudomonas sp.

-

-

667257

-

-

?

hex-2-enoyl-CoA + NADPH

3-hydroxyhexanoyl-CoA + NADP+

Pseudomonas sp.

Q4AE87

-

667257

-

-

?

oct-2-enoyl-CoA + NADPH

3-hydroxyoctanoyl-CoA + NADP+

Escherichia coli

-

-

667257

-

-

?

oct-2-enoyl-CoA + NADPH

3-hydroxyoctanoyl-CoA + NADP+

Homo sapiens

-

-

669416

-

-

?

oct-2-enoyl-CoA + NADPH

3-hydroxyoctanoyl-CoA + NADP+

Pseudomonas sp.

-, Q4AE87

-

667257

-

-

?

propiophenone + NADPH

? + NADP+

Synechococcus elongatus PCC 7942

Q31QF3

123% relative activity compared to acetophenone

701783

-

-

?

propyl acetoacetate + NADPH

? + NADP+

Escherichia coli

-

as substrate it is limited to its weak solubility

704337

-

-

?

methyl acetoacetate + NADPH

? + NADP+

Escherichia coli

-

is not a good substrate as ethyl acetoacetate

704337

-

-

?

additional information

?

-

Escherichia coli

-

only D-(-) isomer is oxidized, beta-ketoacyl thioesters of CoA and pantetheine metabolized at slower rates

285671

-

-

-

additional information

?

-

Brassica napus, Persea americana

-

animals, yeast and Mycobacteria have fatty acid synthetase containing all the individual activities on one or two multifunctional polypeptide chains, plants and most bacterial systems, including E. coli, possess individual monofunctional enzymes and a separate acyl carrier protein

285670

-

-

-

additional information

?

-

Escherichia coli

-

equally active on beta-ketoacyl-ACP derivatives of C4 to C16, broad specificity for chain length of substrates

285671

-

-

-

additional information

?

-

Escherichia coli

-

broad specificity for chain length of substrates

285672

-

-

-

additional information

?

-

Escherichia coli

-

L-isomer completely inactive

285668, 285671

-

-

-

additional information

?

-

Spinacia oleracea

-

L-isomer completely inactive

285668

-

-

-

additional information

?

-

Escherichia coli

-

catalyzes also the reduction of the beta-keto group of unsaturated acyl chains during biosynthesis of unsaturated fatty acids

285683

-

-

-

additional information

?

-

Lactococcus lactis

-

FabG1 is responsible for the first reductive step of the fatty acid synthetic cycle. FabG2 is inactive in fatty acid synthesis

654725

-

-

-

additional information

?

-

Pseudomonas aeruginosa

-

the beta-ketoacyl acyl carrier protein reductase activity of the fatty acid biosynthetic pathway is a determining factor of 3-oxo-homoserine lactone acyl chain lengths

656723

-

-

-

additional information

?

-

Pseudomonas aeruginosa

-

the enzyme is essential in the rhamnolipid biosynthetic pathway of Pseudomonas aeruginosa

669470

-

-

-

additional information

?

-

Homo sapiens

-

the enzyme is part of the fatty acid synthase multienzyme complex, and shares probably a single active site with the enoyl reductase activity of the complex

669416

-

-

-

additional information

?

-

Escherichia coli, Pseudomonas sp.

-

FabG substrate specificity overview

667257

-

-

-

additional information

?

-

Pseudomonas sp.

Q4AE87

FabG substrate specificity overview

667257

-

-

-

additional information

?

-

Pseudomonas putida

A5HIF6, -

fabG overexpression tends to depress the biosynthesis of medium-chain-length polyhydroxyalkanoate due to the reversible conversion of (R)-3-hydroxyalkanoate monomer units into 3-ketoacyl-CoA

693287

-

-

-

additional information

?

-

Mycobacterium tuberculosis

O53665, P0A5Y4

FabG1 is able to act on shorter (C4) acyl substrates

705747

-

-

-

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

3-oxoacyl-[acyl-carrier protein] + NADPH + H+

Pseudomonas putida

A5HIF6, -

beta-oxidation pathway

693287

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Escherichia coli

-

-

285683

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Brassica napus

-

enzyme is part of the dissociable fatty acid synthase type II multienzyme complex for de novo synthesis of fatty acids from acetyl-CoA and malonyl-CoA

285683

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Escherichia coli

-

first reduction step of fatty acid synthase

285671

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Brassica napus, Persea americana

-

first reduction step of fatty acid synthase

285670

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Spinacia oleracea

-

enzyme in fatty acid synthesis

285677

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Brassica napus

-

enzyme in fatty acid synthesis

285683

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Carthamus tinctorius

-

enzyme in fatty acid synthesis

285669

-

-

?

3-oxoacyl-[acyl-carrier protein] + NADPH

3-hydroxyacyl-[acyl-carrier protein] + NADP+

Mycobacterium tuberculosis

-

-

667708

-

-

r

3-oxoacyl-[acyl-carrier protein] + NADPH

3-hydroxyacyl-[acyl-carrier protein] + NADP+

Plasmodium falciparum

-

-

668574

-

-

r

acetoacetyl-CoA + NADPH

3-hydroxybutyryl-CoA + NADP+

Streptococcus pneumoniae

-

-

667646

-

-

?

beta-ketoacyl-[acyl-carrier protein] + NADPH

beta-hydroxyacyl-[acyl-carrier protein] + NADP+

Salmonella enterica subsp. enterica serovar Typhimurium, Escherichia coli

-

first reductive step in the elongation cycle of fatty acid biosynthesis

655871

-

-

?

additional information

?

-

Escherichia coli

-

catalyzes also the reduction of the beta-keto group of unsaturated acyl chains during biosynthesis of unsaturated fatty acids

285683

-

-

-

additional information

?

-

Lactococcus lactis

-

FabG1 is responsible for the first reductive step of the fatty acid synthetic cycle. FabG2 is inactive in fatty acid synthesis

654725

-

-

-

additional information

?

-

Pseudomonas aeruginosa

-

the beta-ketoacyl acyl carrier protein reductase activity of the fatty acid biosynthetic pathway is a determining factor of 3-oxo-homoserine lactone acyl chain lengths

656723

-

-

-

additional information

?

-

Pseudomonas aeruginosa

-

the enzyme is essential in the rhamnolipid biosynthetic pathway of Pseudomonas aeruginosa

669470

-

-

-

additional information

?

-

Homo sapiens

-

the enzyme is part of the fatty acid synthase multienzyme complex, and shares probably a single active site with the enoyl reductase activity of the complex

669416

-

-

-

additional information

?

-

Pseudomonas putida

A5HIF6, -

fabG overexpression tends to depress the biosynthesis of medium-chain-length polyhydroxyalkanoate due to the reversible conversion of (R)-3-hydroxyalkanoate monomer units into 3-ketoacyl-CoA

693287

-

-

-

NADH

Spinacia oleracea

-

NADPH is much more effective than NADH

285668, 285677

2D-image

NADP+

Spinacia oleracea

-

-

285668

2D-image

NADP+

Escherichia coli

-

-

285668, 285681

2D-image

NADP+

Mycobacterium tuberculosis

-

-

667708

2D-image

NADP+

Plasmodium falciparum

-

cooperative transitions in the enzyme due to cofactor and [acyl-carrier-protein] binding, cooperative cofactor binding

668574

2D-image

NADP+

Pseudomonas putida

A5HIF6, -

-

693287

2D-image

NADPH

Persea americana

-

absolute specificity for NADPH

285670

2D-image

NADPH

Brassica napus

-

-

285673

2D-image

NADPH

Brassica juncea

-

-

285675

2D-image

NADPH

Carthamus tinctorius

-

-

285669, 285675

2D-image

NADPH

Cuphea lutea, Pisum sativum

-

-

285675

2D-image

NADPH

Spinacia oleracea

-

-

285668, 285675

2D-image

NADPH

Euglena gracilis

-

-

285676

2D-image

NADPH

Spinacia oleracea

-

much more effective than NADH

285677

2D-image

NADPH

Persea americana

-

-

285674, 285675, 285678

2D-image

NADPH

Cuphea lanceolata

-

-

285679

2D-image

NADPH

Brassica napus

-

absolute specificity for NADPH

285670, 285683

2D-image

NADPH

Escherichia coli

-

absolute specificity for NADPH

285672, 285683

2D-image

NADPH

Plasmodium falciparum

Q965D6

exhibits negative cooperativity for its interaction with the enzyme

654432

2D-image

NADPH

Salmonella enterica subsp. enterica serovar Typhimurium

-

-

655871

2D-image

NADPH

Pseudomonas aeruginosa

-

-

656723

2D-image

NADPH

Pseudomonas sp.

Q4AE87

-

667257

2D-image

NADPH

Plasmodium falciparum

Q86RB1

cofactor binding structure and mechanism

667520

2D-image

NADPH

Streptococcus pneumoniae

-

-

667646

2D-image

NADPH

Mycobacterium tuberculosis

-

-

667708

2D-image

NADPH

Plasmodium falciparum

-

cooperative transitions in the enzyme due to cofactor and [acyl-carrier-protein] binding, cooperative allosteric cofactor binding in presence of [acyl-carrier-protein]

668574

2D-image

NADPH

Homo sapiens

-

-

669416

2D-image

NADPH

Mycobacterium tuberculosis

-

positive cooperativity in binding to enzyme

684702

2D-image

NADPH

Plasmodium falciparum K1

-

-

689435

2D-image

NADPH

Haloarcula hispanica

-

dependent on

701808

2D-image

NADPH

Plasmodium falciparum

-

3fold enhancement in acyl-carrier protein binding to wild-type in the presence of NADPH

703630

2D-image

NADPH

synthetic construct

-

-

704843

2D-image

NADPH

Escherichia coli

-

-

285668, 285671, 655871, 667257, 669013, 703163, 704337, 706142

2D-image

NADPH

Plasmodium falciparum

-

-

670774, 706639

2D-image

additional information

Euglena gracilis

-

NADH is used by NADH-dependent 3-oxoacyl [ACP]-reductase, EC 1.1.1.121

285676

-

additional information

Persea americana

-

NADH is used by NADH-dependent 3-oxoacyl [ACP]-reductase, EC 1.1.1.121

285678

-

additional information

Plasmodium falciparum

Q86RB1

the enzyme activity is maximal at a ionic strength of 325 mM

667520

(E)-1-(4-methylpiperidin-1-yl)-3-phenylprop-2-en-1-one

Escherichia coli

P0AEK2

80% residual activity

703163

2D-image

(E)-2-nitrophenyl cinnamate

Escherichia coli

P0AEK2

-

703163

2D-image

(E)-3-phenoxybenzyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate

Escherichia coli

P0AEK2

61% residual activity

703163

2D-image

(E)-4-cyanophenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate

Escherichia coli

P0AEK2

-

703163

2D-image

(E)-benzyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate

Escherichia coli

P0AEK2

71% residual activity

703163

2D-image

(E)-phenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate

Escherichia coli

P0AEK2

69% residual activity

703163

2D-image

1,2,3,4,6-penta-O-galloyl-beta-D-glucose

Escherichia coli

-

mixed type of inhibition. IC50 value 0.9 microgramm per ml, Ki value 0.21 microgramm per ml

685704

2D-image

1,2,3,4,6-penta-O-galloyl-beta-D-glucose

Pseudomonas aeruginosa

-

minimal inhibitory concentration 0.125 mg/ml

685704

2D-image

1,2,3,4,6-penta-O-galloyl-beta-D-glucose

Staphylococcus epidermidis

-

minimal inhibitory concentration 0.25 mg/ml

685704

2D-image

3-hydroxybutyryl-CoA

Plasmodium falciparum

Q86RB1

competitive product inhibition

667520

2D-image

3-hydroxybutyryl-CoA

Mycobacterium tuberculosis

-

noncompetitive product inhibition pattern

667708

2D-image

4-acetoxyanthecotulide

Plasmodium falciparum K1

-

most active linear sesquiterpene lactone from Anthemis auriculata against FabG

689435

2D-image

4-hydroxyanthecotulide

Plasmodium falciparum K1

-

linear sesquiterpene lactone from Anthemis auriculata

689435

2D-image

Acyl carrier protein

Lactococcus lactis

-

beta-ketobutyryl-CoA reductase activity of FabG1 is inhibited, activity of FabG2 is unaffected

654725

2D-image

acyl-carrier protein

Plasmodium falciparum

-

inhibition of wild-type with increasing concentrations

703630

2D-image

anthecotulide

Plasmodium falciparum K1

-

linear sesquiterpene lactone from Anthemis auriculata

689435

2D-image

Bithionol

Plasmodium falciparum

Q86RB1

over 75% inhibition at 0.02 mM, IC50: 0.010 mM

667520

2D-image

bromochlorophen

Plasmodium falciparum

Q86RB1

an anthelmintic agent, over 75% inhibition at 0.02 mM, IC50: 0.0154 mM

667520

2D-image

chlorogenic acid

Escherichia coli

-

IC50: 0.881-0.948 mM

669013

2D-image

di-resorcinol sulfide

Plasmodium falciparum

Q86RB1

over 75% inhibition at 0.02 mM, IC50: 0.0038 mM

667520

2D-image

DL-3-hydroxybutyryl-CoA

Streptococcus pneumoniae

-

competitive product inhibition

667646

2D-image

epigallocatechin gallate

Escherichia coli

-

-

704337

2D-image

Guanidinium chloride

Plasmodium falciparum

-

FabG is fully unfolded at 4 M, approximately 90% of the enzyme activity can be recovered on dialyzing the denaturant. In presence of NADPH, there is no stabilization of FabG in case of equilibrium unfolding with guanidinium chloride

706639

2D-image

Hexachlorophene

Plasmodium falciparum

Q86RB1

an anthelmintic and antimicrobial agent, over 75% inhibition at 0.02 mM, IC50: 0.002 mM

667520

2D-image

N-(1,3-dioxoisoindolin-2-yl)-2-oxo-2H-chromene-3-carboxamide

Escherichia coli

P0AEK2

69% residual activity

703163

2D-image

NADP+

Streptococcus pneumoniae

-

competitive product inhibition

667646

2D-image

NADP+

Mycobacterium tuberculosis

-

noncompetitive product inhibition pattern

667708

2D-image

NADP+

Escherichia coli

-

competitive product inhibition

669013

2D-image

p-chloromercuribenzoate

Spinacia oleracea

-

-

285668

2D-image

Phenylglyoxal

Persea americana

-

with NADPH 45% remaining activity, without total loss of activity

285674

2D-image

Phenylglyoxal

Cuphea lanceolata

-

-

285679

2D-image

quercetin

synthetic construct

-

-

704843

2D-image

quercetin

Escherichia coli

-

-

706142

2D-image

RNAi

Arabidopsis thaliana

-

suppresses KCR activity, which results in a reduction of cuticular wax load and affects very-long-chain fatty acid composition of sphingolipids, seed triacylglycerols, and root glycerolipids. RNAi-suppressed Arabidopsis KCR1 plants display fused vegetative and reproductive organs, an abnormal root morphology and have abnormal trichome and epidermal cell morphology

700827

-

Tannic acid

Acinetobacter calcoaceticus, Enterobacter aerogenes, Enterobacter cloacae, Enterococcus sp.

-

-

706142

2D-image

Tannic acid

Escherichia coli

-

; strongest inhibition on FabG, shows time-dependent irreversible inhibition

706142

2D-image

Tannic acid

Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Typhi, Salmonella enterica subsp. enterica serovar Typhimurium, Serratia marcescens, Shigella dysenteriae, Shigella flexneri, Shigella sonnei

-

-

706142

2D-image

Tannic acid

Staphylococcus aureus, Staphylococcus epidermidis

-

displays very strong inhibition

706142

2D-image

Urea

Plasmodium falciparum

-

FabG is fully unfolded at 6 M, approximately 90% of the enzyme activity can be recovered on dialyzing the denaturant. Two states in the reversible unfolding process of FabG in presence of NADPH, one is an activity-enhanced state and the other, an inactive state in case of equilibrium unfolding with urea

706639

2D-image

kaempferol

synthetic construct

-

-

704843

2D-image

additional information

Spinacia oleracea

-

not: N-ethylmaleimide, arsenite

285668

-

additional information

Plasmodium falciparum

Q86RB1

no inhibition by triclosan

667520

-

additional information

Escherichia coli

P0AEK2

cinnamic acid derivatives can be accommodated in the substrate-binding region of the active site, above the nicotinamide moiety of the NADPH cofactor

703163

-

additional information

Pseudomonas aeruginosa

-

FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum). Minimum inhibitory concentration is above 1.28 mM

704843

-

additional information

Staphylococcus aureus

-

FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum). Minimum inhibitory concentration is 0.32-0.64 mM

704843

-

additional information

Streptococcus pneumoniae

-

FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum). Minimum inhibitory concentration is 0.01-0.02 mM

704843

-

additional information

Streptococcus sp.

-

FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum). Minimum inhibitory concentration is 0.025-0.05 mM

704843

-

additional information

synthetic construct

-

FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum), strongest inhibition with 40% ethanol extract of galangal. Inhibition is consisted of both reversible and irreversible inhibition. Inhibits FabG in a competitive pattern against NADPH, irreversible inhibition presents two phases (slow and fast one)

704843

-

additional information

Acinetobacter calcoaceticus, Enterobacter aerogenes, Enterobacter cloacae, Enterococcus sp.

-

inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge

706142

-

additional information

Escherichia coli

-

inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge; inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge. Leaf extracts of Acer saccharum and Acer truncatum Bunge display time-dependent irreversible inhibition of FabG, whereas leaf extracts of Acer platanoides, Acer campestre and Acer rubrum show reversible inhibition

706142

-

additional information

Klebsiella pneumoniae

-

inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge

706142

-

additional information

Proteus vulgaris

-

inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge. Is inhibited by the leaf extracts from all five kinds of maples more effectively than are other Gram-negative bacteria strains

706142

-

additional information

Pseudomonas aeruginosa

-

inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge

706142

-

additional information

Salmonella enterica subsp. enterica serovar Typhi

-

inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge. Is inhibited by the leaf extracts from all five kinds of maples more effectively than are other Gram-negative bacteria strains

706142

-

additional information

Salmonella enterica subsp. enterica serovar Typhimurium, Serratia marcescens

-

inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge

706142

-

additional information

Shigella dysenteriae

-

inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge. Is inhibited by the leaf extracts from all five kinds of maples more effectively than are other Gram-negative bacteria strains

706142

-

additional information

Shigella flexneri, Shigella sonnei, Staphylococcus aureus

-

inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge

706142

-

additional information

Staphylococcus epidermidis

-

inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge, except strain 04-5

706142

-

additional information

Synechococcus elongatus PCC 7942

Q31QF3

variation of the buffer molarity between 25 mM and 200 mM or addition of NaCl up to 500 mM has no significant effect on enzyme activity

701783

-

additional information

Plasmodium falciparum

-

affinity of acyl-carrier protein for FabG is increased by 3fold in the presence of 1 M urea

706639

-

89.2

-

1,1,1-trifluoroacetone

Synechococcus elongatus PCC 7942

Q31QF3

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

2D-image

5.72

-

2',3',4',5',6'-pentafluoroacetophenone

Synechococcus elongatus PCC 7942

Q31QF3

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

2D-image

7.38

-

3'-fluoroacetophenone

Synechococcus elongatus PCC 7942

Q31QF3

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

2D-image

14.34

-

4'-chloroacetophenone

Synechococcus elongatus PCC 7942

Q31QF3

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

2D-image

12.29

-

4'-fluoroacetophenone

Synechococcus elongatus PCC 7942

Q31QF3

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

2D-image

0.0012

-

acetoacetyl-CoA

Homo sapiens

-

pH 7.4, 25°C, recombinant mutant K1699Q

669416

2D-image

0.002

-

acetoacetyl-CoA

Homo sapiens

-

pH 7.4, 25°C, recombinant mutant K1699A

669416

2D-image

0.01

-

acetoacetyl-CoA

Homo sapiens

-

pH 7.4, 25°C, recombinant wild-type enzyme

669416

2D-image

0.075

-

acetoacetyl-CoA

Plasmodium falciparum

Q965D6

pH 7.5

654432

2D-image

0.165

-

acetoacetyl-CoA

Mycobacterium tuberculosis

-

pH 7.6, 30°C

667708

2D-image

0.25

-

acetoacetyl-CoA

Spinacia oleracea

-

-

285668

2D-image

0.261

-

acetoacetyl-CoA

Brassica napus

-

-

285673

2D-image

0.43

-

acetoacetyl-CoA

Plasmodium falciparum

-

pH 6.8, 22°C, recombinant enzyme

668574

2D-image

0.43

-

acetoacetyl-CoA

Plasmodium falciparum

-

wild-type

703630

2D-image

0.44

-

acetoacetyl-CoA

Plasmodium falciparum

-

mutant R230A

703630

2D-image

0.45

-

acetoacetyl-CoA

Plasmodium falciparum

-

mutant R187A

703630

2D-image

0.47

-

acetoacetyl-CoA

Persea americana

-

-

285674

2D-image

0.47

-

acetoacetyl-CoA

Plasmodium falciparum

-

mutant R187A/R230A; mutant R187E

703630

2D-image

0.49

-

acetoacetyl-CoA

Plasmodium falciparum

-

mutant R230E

703630

2D-image

0.51

-

acetoacetyl-CoA

Plasmodium falciparum

Q86RB1

pH 6.8, 25°C, recombinant enzyme

667520

2D-image

0.58

-

acetoacetyl-CoA

Plasmodium falciparum

-

mutant R187E/R230E

703630

2D-image

2.2

-

acetoacetyl-CoA

Streptococcus pneumoniae

-

pH 7.6, 30°C

667646

2D-image

48

-

acetoacetyl-N-acetylcysteamine

Persea americana

-

-

285674

2D-image

0.003

-

acetoacetyl-[acyl-carrier protein]

Brassica napus

-

-

285673

2D-image

0.0037

-

acetoacetyl-[acyl-carrier protein]

Spinacia oleracea

-

-

285668

2D-image

0.0079

-

acetoacetyl-[acyl-carrier protein]

Persea americana

-

-

285674

2D-image

0.009

-

acetoacetyl-[acyl-carrier protein]

Carthamus tinctorius

-

-

285669

2D-image

0.017

-

acetoacetyl-[acyl-carrier protein]

Spinacia oleracea

-

-

285677

2D-image

46.92

-

acetophenone

Synechococcus elongatus PCC 7942

Q31QF3

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

2D-image

2.71

-

alpha-chloroacetophenone

Synechococcus elongatus PCC 7942

Q31QF3

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

2D-image

0.29

-

beta-ketobutyryl-CoA

Lactococcus lactis

-

pH 7.0, 30°C, FabG1

654725

2D-image

4.06

-

beta-ketobutyryl-CoA

Lactococcus lactis

-

pH 7.0, 30°C, FabG2

654725

2D-image

0.006

-

crotonyl-CoA

Homo sapiens

-

pH 7.4, 25°C, recombinant wild-type enzyme

669416

2D-image

0.007

-

dodec-2-enoyl-CoA

Homo sapiens

-

pH 7.4, 25°C, recombinant wild-type enzyme

669416

2D-image

8.31

-

ethyl 4-chloroacetoacetate

Synechococcus elongatus PCC 7942

Q31QF3

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

2D-image

0.127

-

ethyl acetoacetate

Escherichia coli

-

-

704337

2D-image

0.035

-

N-acetoacetyl-N-cysteamine

Brassica napus

-

-

285673

-

0.003

-

NADPH

Homo sapiens

-

pH 7.4, 25°C, recombinant wild-type enzyme, with crotonyl-CoA

669416

2D-image

0.005

-

NADPH

Homo sapiens

-

pH 7.4, 25°C, recombinant wild-type enzyme, with acetoacetyl-CoA

669416

2D-image

0.0093

-

NADPH

Persea americana

-

-

285674

2D-image

0.01

-

NADPH

Escherichia coli

-

-

704337

2D-image

0.015

-

NADPH

Spinacia oleracea

-

-

285677

2D-image

0.016

-

NADPH

Carthamus tinctorius

-

-

285669

2D-image

0.023

-

NADPH

Brassica napus

-

+ acetoacetyl-[acyl-carrier-protein]

285673

2D-image

0.025

-

NADPH

Spinacia oleracea

-

-

285668

2D-image

0.026

-

NADPH

Mycobacterium tuberculosis

-

pH 7.6, 30°C

667708

2D-image

0.0412

-

NADPH

Plasmodium falciparum

-

in the presence of 1.5 M urea, pH 7.0, 25°C

706639

2D-image

0.043

-

NADPH

Plasmodium falciparum

-

pH 6.8, 22°C, recombinant enzyme

668574

2D-image

0.0434

-

NADPH

Plasmodium falciparum

-

in the presence of 1 M urea, pH 7.0, 25°C

706639

2D-image

0.0461

-

NADPH

Plasmodium falciparum

-

without urea, pH 7.0, 25°C

706639

2D-image

0.0475

-

NADPH

Plasmodium falciparum

Q86RB1

pH 6.8, 25°C, recombinant enzyme

667520

2D-image

0.052

-

NADPH

Homo sapiens

-

pH 7.4, 25°C, recombinant mutant K1699A, with acetoacetyl-CoA

669416

2D-image

0.1

-

NADPH

Homo sapiens

-

pH 7.4, 25°C, recombinant wild-type enzyme, with octenoyl-CoA

669416

2D-image

0.123

-

NADPH

Homo sapiens

-

pH 7.4, 25°C, recombinant mutant K1699Q, with acetoacetyl-CoA

669416

2D-image

0.4

-

NADPH

Streptococcus pneumoniae

-

pH 7.6, 30°C

667646

2D-image

0.4553

-

NADPH

Plasmodium falciparum

-

in the presence of 4 M urea, pH 7.0, 25°C

706639

2D-image

0.54

-

NADPH

Homo sapiens

-

pH 7.4, 25°C, recombinant wild-type enzyme, with dodecenoyl-CoA

669416

2D-image

6.03

-

propiophenone

Synechococcus elongatus PCC 7942

Q31QF3

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

2D-image

1.92

-

ethylbenzoylacetate

Synechococcus elongatus PCC 7942

Q31QF3

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

2D-image

additional information

-

additional information

Spinacia oleracea

-

-

285677

-

additional information

-

additional information

Persea americana

-

-

285678

-

additional information

-

additional information

Plasmodium falciparum

Q86RB1

kinetic analysis of the recombinant enzyme, kinetic mechanism

667520

-

additional information

-

additional information

Streptococcus pneumoniae

-

detailed steady-state kinetic analysis, solvent and multiple kinetic isotope effects, random bi bi kinetic mechanism

667646

-

additional information

-

additional information

Mycobacterium tuberculosis

-

detailed kinetic and thermodynamic analysis, solvent and multiple kinetic isotope effects, kinetic mechanism

667708

-

additional information

-

additional information

Plasmodium falciparum

-

cooperativity kinetics, binding constants of substrates and cofactors

668574

-

0.014

-

acetoacetyl-CoA

Plasmodium falciparum

Q965D6

pH 7.5

654432

2D-image

0.33

-

acetoacetyl-CoA

Homo sapiens

-

pH 7.4, 25°C, recombinant mutant K1699A

669416

2D-image

0.43

-

acetoacetyl-CoA

Homo sapiens

-

pH 7.4, 25°C, recombinant mutant K1699Q

669416

2D-image

4

-

acetoacetyl-CoA

Homo sapiens

-

pH 7.4, 25°C, recombinant wild-type enzyme

669416

2D-image

7

-

acetoacetyl-CoA

Mycobacterium tuberculosis

-

pH 7.0, 25°C

667708

2D-image

11

-

acetoacetyl-CoA

Streptococcus pneumoniae

-

pH 7.6, 30°C

667646

2D-image

259

-

acetoacetyl-CoA

Plasmodium falciparum

-

pH 6.8, 22°C, recombinant enzyme

668574

2D-image

265

-

acetoacetyl-CoA

Plasmodium falciparum

Q86RB1

pH 6.8, 25°C, recombinant enzyme

667520

2D-image

0.3

-

ethyl acetoacetate

Escherichia coli

-

-

704337

2D-image

0.33

-

NADPH

Homo sapiens

-

pH 7.4, 25°C, recombinant mutant K1699A

669416

2D-image

0.43

-

NADPH

Homo sapiens

-

pH 7.4, 25°C, recombinant mutant K1699Q

669416

2D-image

0.59

-

NADPH

Escherichia coli

-

-

704337

2D-image

4

-

NADPH

Homo sapiens

-

pH 7.4, 25°C, recombinant wild-type enzyme

669416

2D-image

7

-

NADPH

Mycobacterium tuberculosis

-

pH 7.6, 30°C

667708

2D-image

11

-

NADPH

Streptococcus pneumoniae

-

pH 7.6, 30°C

667646

2D-image

148.6

-

NADPH

Plasmodium falciparum

-

in the presence of 4 M urea, pH 7.0, 25°C

706639

2D-image

187.3

-

NADPH

Plasmodium falciparum

-

in the presence of 1.5 M urea, pH 7.0, 25°C

706639

2D-image

250

-

NADPH

Plasmodium falciparum

-

without urea, pH 7.0, 25°C

706639

2D-image

259

-

NADPH

Plasmodium falciparum

-

pH 6.8, 22°C, recombinant enzyme

668574

2D-image

265

-

NADPH

Plasmodium falciparum

Q86RB1

pH 6.8, 25°C, recombinant enzyme

667520

2D-image

344.7

-

NADPH

Plasmodium falciparum

-

in the presence of 1 M urea, pH 7.0, 25°C

706639

2D-image

0.0102

-

3-hydroxybutyryl-CoA

Plasmodium falciparum

Q86RB1

pH 6.8, 25°C, recombinant enzyme

667520

2D-image

additional information

-

additional information

Mycobacterium tuberculosis

-

product inhibition kinetics, overview

667708

-

additional information

-

additional information

Escherichia coli

-

inhibition kinetic analysis

669013

-

0.45

-

(E)-2-nitrophenyl cinnamate

Escherichia coli

P0AEK2

pH 7.0

703163

2D-image

0.18

-

(E)-4-cyanophenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate

Escherichia coli

P0AEK2

pH 7.0

703163

2D-image

0.18

-

(E)-phenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate

Escherichia coli

P0AEK2

pH 7.0

703163

2D-image

0.5

-

4-acetoxyanthecotulide

Plasmodium falciparum K1

-

pH 7.0, 25°C

689435

2D-image

0.75

-

4-hydroxyanthecotulide

Plasmodium falciparum K1

-

pH 7.0, 25°C

689435

2D-image

1

-

anthecotulide

Plasmodium falciparum K1

-

pH 7.0, 25°C

689435

2D-image

0.01

-

Bithionol

Plasmodium falciparum

Q86RB1

over 75% inhibition at 0.02 mM, IC50: 0.010 mM

667520

2D-image

0.0154

-

bromochlorophen

Plasmodium falciparum

Q86RB1

an anthelmintic agent, over 75% inhibition at 0.02 mM, IC50: 0.0154 mM

667520

2D-image

0.881

0.948

chlorogenic acid

Escherichia coli

-

IC50: 0.881-0.948 mM

669013

2D-image

0.0038

-

di-resorcinol sulfide

Plasmodium falciparum

Q86RB1

over 75% inhibition at 0.02 mM, IC50: 0.0038 mM

667520

2D-image

0.00554

-

epigallocatechin gallate

Escherichia coli

-

at pH 8.0

704337

2D-image

0.0084

-

epigallocatechin gallate

Escherichia coli

-

at pH 8.3

704337

2D-image

0.0105

-

epigallocatechin gallate

Escherichia coli

-

at pH 7.5

704337

2D-image

0.0336

-

epigallocatechin gallate

Escherichia coli

-

at pH 8.5

704337

2D-image

0.0578

-

epigallocatechin gallate

Escherichia coli

-

at pH 7.0

704337

2D-image

0.12

-

epigallocatechin gallate

Escherichia coli

-

at pH 9.0

704337

2D-image

0.1223

-

epigallocatechin gallate

Escherichia coli

-

at pH 6.5

704337

2D-image

0.002

-

Hexachlorophene

Plasmodium falciparum

Q86RB1

an anthelmintic and antimicrobial agent, over 75% inhibition at 0.02 mM, IC50: 0.002 mM

667520

2D-image

0.215

-

N-(1,3-dioxoisoindolin-2-yl)-2-oxo-2H-chromene-3-carboxamide

Escherichia coli

P0AEK2

pH 7.0

703163

2D-image

0.3048

-

quercetin

synthetic construct

-

pH 7.0

704843

2D-image

0.00078

-

Tannic acid

Escherichia coli

-

-

706142

2D-image

0.0212

-

kaempferol

synthetic construct

-

pH 7.0

704843

2D-image

additional information

-

additional information

synthetic construct

-

IC(50) value of 4.47 microg/ml for extract of galangal, pH 7.0

704843

-

0.0017

0.0018

Plasmodium falciparum

-

recombinant refolded enzyme

670774

0.0019

-

Plasmodium falciparum

-

recombinant soluble enzyme

670774

0.0032

-

Escherichia coli

-

-

285668

0.005

-

Spinacia oleracea

-

-

285668

0.0132

-

Pisum sativum

-

-

285675

0.0168

-

Cuphea lutea

-

-

285675

0.0189

-

Carthamus tinctorius

-

-

285675

0.0192

-

Spinacia oleracea

-

-

285675

0.0197

-

Carthamus tinctorius

-

-

285669

0.02

-

Synechococcus elongatus PCC 7942

Q31QF3

with 2-octanone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

0.036

-

Homo sapiens

-

recombinant purified mutant K1699A

669416

0.0366

-

Brassica juncea

-

-

285675

0.043

-

Homo sapiens

-

recombinant purified mutant K1699Q

669416

0.08

-

Synechococcus elongatus PCC 7942

Q31QF3

with 4'-fluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

0.13

-

Synechococcus elongatus PCC 7942

Q31QF3

with 3'-fluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0; with acetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

0.15

-

Synechococcus elongatus PCC 7942

Q31QF3

with 4'-chloroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

0.16

-

Synechococcus elongatus PCC 7942

Q31QF3

with propiophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

0.24

-

Homo sapiens

-

recombinant purified wild-type BKR

669416

1.03

-

Synechococcus elongatus PCC 7942

Q31QF3

with 1,1,1-trifluoroacetone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

1.16

-

Synechococcus elongatus PCC 7942

Q31QF3

with ethylbenzoylacetate as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

2

-

Plasmodium falciparum

-

mutant R187E/R230E, acyl-carrier protein-dependent spectroscopic assay

703630

2.45

-

Synechococcus elongatus PCC 7942

Q31QF3

with alpha-chloroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

3

-

Plasmodium falciparum

-

mutant R187A/R230A, acyl-carrier protein-dependent spectroscopic assay

703630

3.93

-

Synechococcus elongatus PCC 7942

Q31QF3

with 2',3',4',5',6'-pentafluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

4

-

Plasmodium falciparum

-

mutant R230E, acyl-carrier protein-dependent spectroscopic assay

703630

6.1

-

Plasmodium falciparum

-

mutant R230A, acyl-carrier protein-dependent spectroscopic assay

703630

6.3

-

Plasmodium falciparum

-

mutant R187E, acyl-carrier protein-dependent spectroscopic assay

703630

6.9

-

Plasmodium falciparum

-

mutant R187A, acyl-carrier protein-dependent spectroscopic assay

703630

7.95

-

Spinacia oleracea

-

-

285668

38.29

-

Synechococcus elongatus PCC 7942

Q31QF3

with ethyl 4-chloroacetoacetate as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

701783

42.6

-

Plasmodium falciparum

-

mutant R187E/R230E, acyl-carrier protein-independent spectroscopic assay

703630

45.6

-

Plasmodium falciparum

-

mutant R187A/R230A, acyl-carrier protein-independent spectroscopic assay

703630

54.2

-

Plasmodium falciparum

-

mutant R230E, acyl-carrier protein-independent spectroscopic assay

703630

54.5

-

Plasmodium falciparum

-

mutant R187E, acyl-carrier protein-independent spectroscopic assay

703630

55.3

-

Plasmodium falciparum

-

mutant R230A, acyl-carrier protein-independent spectroscopic assay

703630

57.9

-

Plasmodium falciparum

-

mutant R187A, acyl-carrier protein-independent spectroscopic assay

703630

59.8

-

Plasmodium falciparum

-

purified recombinant enzyme

668574

59.8

-

Plasmodium falciparum

-

wild-type, acyl-carrier protein-independent spectroscopic assay

703630

70.6

-

Plasmodium falciparum

-

wild-type, acyl-carrier protein-dependent spectroscopic assay

703630

280

-

Persea americana

-

acetoacetyl-N-acetylcysteamine

285674

450

-

Persea americana

-

acetoacetyl-CoA

285674

530

-

Persea americana

-

acetoacteyl-ACP

285674

3839

-

Cuphea lanceolata

-

recombinant protein, cosubstrate NADH, pH 7.0, phosphate buffer

285679

4930

-

Escherichia coli

-

purified enzyme

285672

9401

-

Persea americana

-

purified enzyme, protein determination by amino acid analysis

285674

83140

-

Cuphea lanceolata

-

cosubstrate NADPH, pH 8.35, carbonate buffer

285679

136000

-

Brassica napus

-

purified enzyme, protein determination by calculation from absorption at 280 nm

285673

180400

-

Cuphea lanceolata

-

recombinant protein, cosubstrate NADPH, pH 7.0, phosphate buffer

285679

additional information

-

Homo sapiens

-

-

669416

additional information

-

Pseudomonas putida

A5HIF6, -

the intrinsic NADPH dependent 3-ketoacyl-CoA reductase activity in the transformant Pseudomonas putida with overexpressed fabG gene increased about 1.6-fold from 0.87 to 1.39 units/mg protein, indicating successful overexpression of the fabGJ genes in Pseudomonas putida KCTC1639.

693287

5.7

6.2

Spinacia oleracea

-

-

285677

6

7

Escherichia coli

-

-

285672

6.5

-

Persea americana

-

-

285678

6.8

-

Plasmodium falciparum

Q86RB1

-

667520

6.8

-

Plasmodium falciparum

-

assay at

668574

7

-

Mycobacterium tuberculosis

-

assay at

667708

7

-

Escherichia coli

-

assay at

669013

7

-

synthetic construct

-

assay conditions

704843

7

-

Mycobacterium tuberculosis

-

assay at

724676

7.4

-

Homo sapiens

-

assay at

669416

7.5

-

Plasmodium falciparum

-

assay at

670774

7.6

-

Streptococcus pneumoniae

-

-

667646

8

-

Escherichia coli

-

assay at

667257

8

-

Pseudomonas sp.

Q4AE87

assay at

667257

8

-

Synechococcus elongatus PCC 7942

Q31QF3

-

701783

8

-

Escherichia coli

-

-

704337

additional information

-

Mycobacterium tuberculosis

-

increase in alpha-secondary deuterium kinetic isotope effect values measured at pH 10 as compared to those obtained at pH 7 points to isotope- and pH-sensitive steps occurring concomitantly

684702

4

10

Synechococcus elongatus PCC 7942

Q31QF3

pH 4: about 60% of maximal activity, pH 10: about 75% of maximal activity

701783

5.5

8.5

Persea americana

-

about 50% of activity maximum at pH 5.5 and 8.5

285678

5.5

9.5

Streptococcus pneumoniae

-

pH profile, detailed mechanistic pH-dependence analysis, overview

667646

5.8

10

Mycobacterium tuberculosis

-

pH profile, pH-dependence analysis, overview

667708

6

8.7

Spinacia oleracea

-

both reaction directions

285668

22

-

Escherichia coli

-

assay at room temperature

667257

22

-

Pseudomonas sp.

Q4AE87

assay at room temperature

667257

22

-

Plasmodium falciparum

-

assay at room temperature

668574

25

-

Plasmodium falciparum

Q86RB1

assay at

667520

25

-

Mycobacterium tuberculosis

-

assay at

667708

25

-

Homo sapiens

-

assay at

669416

25

-

Mycobacterium tuberculosis

-

assay at

724676

30

-

Streptococcus pneumoniae

-

assay at

667646

30

-

Pseudomonas putida

A5HIF6, -

assay at

693287

37

-

Escherichia coli

-

assay at

669013

44

-

Synechococcus elongatus PCC 7942

Q31QF3

-

701783

45

-

Escherichia coli

-

-

704337

15

37

Mycobacterium tuberculosis

-

temperature profile

667708

30

55

Synechococcus elongatus PCC 7942

Q31QF3

30°C: about 55% of maximal activity, 55°C: about 65% of maximal activity

701783

5.9

-

Pseudomonas putida

A5HIF6, -

predicted

693287

cell culture

Pseudomonas putida

A5HIF6, -

strain KCTC1639

693287

embryo

Arabidopsis thaliana

-

KCR1 and KCR2

700827

flower

Cuphea lanceolata

-

-

285679

flower

Arabidopsis thaliana

-

KCR1 and KCR2

700827

inflorescence

Arabidopsis thaliana

-

KCR1 and KCR2 present in inflorescence stem

700827

leaf

Pisum sativum

-

-

285675

leaf

Spinacia oleracea

-

-

285668, 285675, 285677

leaf

Cuphea lanceolata

-

-

285679

leaf

Arabidopsis thaliana

-

KCR1 and KCR2

700827

mesocarp

Persea americana

-

-

285670, 285674, 285678

root

Cuphea lanceolata

-

-

285679

root

Arabidopsis thaliana

-

only KCR1

700827

seed

Brassica juncea

-

-

285675

seed

Carthamus tinctorius

-

-

285669, 285675

seed

Cuphea lutea, Persea americana

-

-

285675

seed

Cuphea lanceolata

-

-

285679

silique

Arabidopsis thaliana

-

KCR1 and KCR2

700827

endoplasmic reticulum

Arabidopsis thaliana

-

KCR1 and KCR2

5783

700827

mitochondrion

Caenorhabditis elegans, Saccharomyces cerevisiae

-

-

5739

704725

soluble

Plasmodium falciparum

Q965D6

recombinant enzyme from Escherichia coli

-

654432

soluble

Mycobacterium tuberculosis

O53665, P0A5Y4

-

-

705747

soluble

Saccharomyces cerevisiae

-

-

-

705747

4nbt, download

SCOP (4nbt)

CATH (4nbt)

Acholeplasma laidlawii (strain PG-8A)

2p68, download

SCOP (2p68)

CATH (2p68)

Aquifex aeolicus (strain VF5)

2pnf, download

SCOP (2pnf)

CATH (2pnf)

Aquifex aeolicus (strain VF5)

2uvd, download

SCOP (2uvd)

CATH (2uvd)

Bacillus anthracis

4j1q, download

SCOP (4j1q)

CATH (4j1q)

Bacillus subtilis (strain 168)

4j1s, download

SCOP (4j1s)

CATH (4j1s)

Bacillus subtilis (strain 168)

1edo, download

SCOP (1edo)

CATH (1edo)

Brassica napus

3emk, download

SCOP (3emk)

CATH (3emk)

Brucella abortus (strain 2308)

3enn, download

SCOP (3enn)

CATH (3enn)

Brucella abortus (strain 2308)

3n74, download

SCOP (3n74)

CATH (3n74)

Brucella abortus (strain 2308)

4one, download

SCOP (4one)

CATH (4one)

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)

4o6v, download

SCOP (4o6v)

CATH (4o6v)

Brucella suis (strain ATCC 23445 / NCTC 10510)

4gkb, download

SCOP (4gkb)