BRENDA - Enzyme Database show
show all sequences of 1.1.1.100

Identification of the polyhydroxyalkanoate (PHA)-specific acetoacetyl coenzyme A reductase among multiple FabG paralogs in Haloarcula hispanica and reconstruction of the PHA biosynthetic pathway in Haloferax volcanii

Han, J.; Lu, Q.; Zhou, L.; Liu, H.; Xiang, H.; Appl. Environ. Microbiol. 75, 6168-6175 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
heterologous coexpression of the polyhydroxyalkanoate synthase genes phaEC together with fabG1 in Haloferax volcanii
Haloarcula hispanica
heterologous coexpression of the polyhydroxyalkanoate synthase genes phaEC together with Haloarcula hispanica fabG1 reconstructs the polyhydroxyalkanoate biosynthetic pathway
Haloferax volcanii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Haloarcula hispanica
-
strain AS2049
-
Haloarcula hispanica AS2049
-
strain AS2049
-
Haloferax volcanii
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
dependent on
Haloarcula hispanica
Cloned(Commentary) (protein specific)
Commentary
Organism
heterologous coexpression of the polyhydroxyalkanoate synthase genes phaEC together with fabG1 in Haloferax volcanii
Haloarcula hispanica
heterologous coexpression of the polyhydroxyalkanoate synthase genes phaEC together with Haloarcula hispanica fabG1 reconstructs the polyhydroxyalkanoate biosynthetic pathway
Haloferax volcanii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
dependent on
Haloarcula hispanica
General Information
General Information
Commentary
Organism
malfunction
DELTAfabG1 cells lose the ability to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate). When the DELTAfabG1 strain is transformed with the fabG1 expression plasmid pWLG1, the capability of polyhydroxyalkanoate accumulation is restored. When the fabG1 gene is disrupted or knocked out, the NADPH-dependent activity of acetoacetyl-CoA reductase in the mutant, the DELTAfabG1 or fabG1-1 strain, is greatly decreased
Haloarcula hispanica
malfunction
polyhydroxyalkanoate-defective haloarchaeon
Haloferax volcanii
physiological function
FabG1 has the function of supplying precursors for polyhydroxyalkanoate biosynthesis
Haloarcula hispanica
General Information (protein specific)
General Information
Commentary
Organism
malfunction
DELTAfabG1 cells lose the ability to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate). When the DELTAfabG1 strain is transformed with the fabG1 expression plasmid pWLG1, the capability of polyhydroxyalkanoate accumulation is restored. When the fabG1 gene is disrupted or knocked out, the NADPH-dependent activity of acetoacetyl-CoA reductase in the mutant, the DELTAfabG1 or fabG1-1 strain, is greatly decreased
Haloarcula hispanica
malfunction
polyhydroxyalkanoate-defective haloarchaeon
Haloferax volcanii
physiological function
FabG1 has the function of supplying precursors for polyhydroxyalkanoate biosynthesis
Haloarcula hispanica
Other publictions for EC 1.1.1.100
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735600
Zhao
In vitro inhibition of fatty a ...
Homo sapiens
Biochem. Biophys. Res. Commun.
445
346-351
2014
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735850
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Inhibitory effects of grape sk ...
Gallus gallus
BMC Complement. Altern. Med.
13
361
2013
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724676
Rosado
Role of Serine140 in the mode ...
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BMC Res. Notes
5
526
2012
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723843
Subramanian
Structure of 3-ketoacyl-(acyl- ...
Rickettsia prowazekii
Acta Crystallogr. Sect. F
67
1118-1122
2011
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700827
Beaudoin
Functional characterization of ...
Arabidopsis thaliana
Plant Physiol.
150
1174-1191
2009
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701808
Han
Identification of the polyhydr ...
Haloarcula hispanica, Haloarcula hispanica AS2049, Haloferax volcanii
Appl. Environ. Microbiol.
75
6168-6175
2009
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703163
Kristan
Novel inhibitors of beta-ketoa ...
Escherichia coli
Chem. Biol. Interact.
178
310-316
2009
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1
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704725
Gurvitz
Caenorhabditis elegans F09E10. ...
Caenorhabditis elegans, Saccharomyces cerevisiae
J. Biomed. Biotechnol.
FEHLT
0000
2009
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705747
Gurvitz
The essential mycobacterial ge ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Saccharomyces cerevisiae
Mol. Genet. Genomics
282
407-416
2009
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9
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706142
Wu
-
Inhibitory effects on bacteria ...
Acinetobacter calcoaceticus, Acinetobacter calcoaceticus 25001, Enterobacter aerogenes, Enterobacter aerogenes 45102, Enterobacter cloacae, Enterobacter cloacae 45301, Enterococcus sp., Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Typhi, Salmonella enterica subsp. enterica serovar Typhi H901, Salmonella enterica subsp. enterica serovar Typhimurium, Serratia marcescens, Serratia marcescens 41002, Shigella dysenteriae, Shigella flexneri, Shigella sonnei, Shigella sonnei 51592, Staphylococcus aureus, Staphylococcus epidermidis
Phytother. Res.
24
535-541
2009
-
16
1
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33
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684702
Silva
Mycobacterium tuberculosis bet ...
Mycobacterium tuberculosis
Arch. Biochem. Biophys.
471
1-10
2008
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685704
Zhang
The antibacterial efficacy of ...
Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis
Biotechnol. Appl. Biochem.
51
73-78
2008
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685775
Nomura
FabG mediates polyhydroxyalkan ...
Escherichia coli
Biotechnol. Prog.
24
342-351
2008
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1
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689435
Karioti
Inhibiting enoyl-ACP reductase ...
Plasmodium falciparum K1
Phytomedicine
15
1125-1129
2008
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5
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693287
Vo
Comparative effect of overexpr ...
Pseudomonas putida
J. Biosci. Bioeng.
106
95-98
2008
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701783
Hoelsch
Identification, cloning, and c ...
Synechococcus elongatus PCC 7942
Appl. Environ. Microbiol.
74
6697-6702
2008
1
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1
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11
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1
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703630
Karmodiya
Deciphering the key residues i ...
Plasmodium falciparum
FEBS J.
275
4756-4766
2008
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6
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1
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704337
Sun
A substitutive substrate for m ...
Escherichia coli
J. Biochem. Biophys. Methods
70
850-856
2008
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704843
Huang
Antimicrobial effect by extrac ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa ATCC27853, Staphylococcus aureus, Staphylococcus aureus ATCC25923, Streptococcus pneumoniae, Streptococcus sp., synthetic construct
J. Enzyme Inhib. Med. Chem.
23
362-368
2008
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706639
Karmodiya
A unique and differential effe ...
Plasmodium falciparum
Proteins Struct. Funct. Genet.
70
528-538
2008
1
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4
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706640
Zaccai
Crystal structure of a 3-oxoac ...
Bacillus anthracis
Proteins Struct. Funct. Genet.
70
562-567
2008
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1
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684165
Mao
Crystallization and X-ray diff ...
Aquifex aeolicus VF5
Acta Crystallogr. Sect. F
63
106-109
2007
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1
1
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4
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1
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687216
Karmodiya
Conformational stability and t ...
Plasmodium falciparum
IUBMB Life
59
441-449
2007
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689500
OHara
Antisense expression of 3-oxoa ...
Brassica napus
Plant Cell Physiol.
48
736-744
2007
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1
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1
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Only one of the two annotated ...
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Poletto
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Shimakata
The purification and function ...
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Shimakata
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Shimakata
Fatty acid synthetase of Spina ...
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Caughey
The characteristics of some co ...
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4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-