Cloned (Comment) | Organism |
---|---|
pET15b-fabG vector construct transformed into the Escherichia coli BL21 DE3 strain | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(E)-1-(4-methylpiperidin-1-yl)-3-phenylprop-2-en-1-one | 80% residual activity | Escherichia coli | |
(E)-2-nitrophenyl cinnamate | - |
Escherichia coli | |
(E)-3-phenoxybenzyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate | 61% residual activity | Escherichia coli | |
(E)-4-cyanophenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate | - |
Escherichia coli | |
(E)-benzyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate | 71% residual activity | Escherichia coli | |
(E)-phenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate | 69% residual activity | Escherichia coli | |
additional information | cinnamic acid derivatives can be accommodated in the substrate-binding region of the active site, above the nicotinamide moiety of the NADPH cofactor | Escherichia coli | |
N-(1,3-dioxoisoindolin-2-yl)-2-oxo-2H-chromene-3-carboxamide | 69% residual activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AEK2 | - |
- |
Purification (Comment) | Organism |
---|---|
by sonication, centrifugation and on Ni-NTA column | Escherichia coli |
Storage Stability | Organism |
---|---|
-80°C, 10% glycerol | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + NADPH + H+ | - |
Escherichia coli | D-beta-hydroxybutyryl-CoA + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl-ACP reductase | - |
Escherichia coli |
beta-ketoacyl-[acyl carrier protein] reductase | - |
Escherichia coli |
FabG | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.18 | - |
pH 7.0 | Escherichia coli | (E)-phenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate | |
0.18 | - |
pH 7.0 | Escherichia coli | (E)-4-cyanophenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate | |
0.215 | - |
pH 7.0 | Escherichia coli | N-(1,3-dioxoisoindolin-2-yl)-2-oxo-2H-chromene-3-carboxamide | |
0.45 | - |
pH 7.0 | Escherichia coli | (E)-2-nitrophenyl cinnamate |