6.5.1.6: DNA ligase (ATP or NAD+)

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For detailed information about DNA ligase (ATP or NAD+), go to the full flat file.

Reaction

5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m

Synonyms

Tfu DNA ligase

ECTree

6 Ligases

6.5 Forming phosphoric-ester bonds

6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)

6.5.1.6 DNA ligase (ATP or NAD+)

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Results	in table
107	AA Sequence
2	Cloned(Commentary)
4	Inhibitors
2	KM Value [mM]
5	Metals/Ions
1	Molecular Weight [Da]
2	Organism
1	PDB ID
3	pH Optimum
2	pH Range
2	Purification (Commentary)
11	Reaction
2	Reference
8	Substrates and Products (Substrate)
1	Subunits
1	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
2	Temperature Range [°C]
2	Temperature Stability [°C]

Reaction

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Reaction on EC 6.5.1.6 - DNA ligase (ATP or NAD+)

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(deoxyribonucleotide)n-3'-hydroxyl + 5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP

3 entries

5'-adenosyl [DNA ligase]-Nepsilon-phosphono-L-lysine + 5'-phospho-(deoxyribonucleotide)m = 5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m + [DNA ligase]-L-lysine

2 entries

ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide

2 entries

ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate

2 entries

ATP + [DNA ligase]-L-lysine = 5'-adenosyl [DNA ligase]-Nepsilon-phosphono-L-lysine + diphosphate

(1a)

NAD+ + [DNA ligase]-L-lysine = 5'-adenosyl [DNA ligase]-Nepsilon-phosphono-L-lysine + beta-nicotinamide D-nucleotide

(2a)